[1]廖霞,杨华,刘秋雁,等.青风藤果浆萃取物对痤疮主要致病菌的体外抑菌活性[J].中国皮肤性病学杂志,2021,(09):969-974.[doi:10.13735/j.cjdv.1001-7089.202103120]
 LIAO Xia,YANG Hua,LIU Qiuyan,et al.In Vitro Bacteriostasis of Fruit Pulp Extract of Caulis Sinomenii on the Main Pathogens of Acne[J].The Chinese Journal of Dermatovenereology,2021,(09):969-974.[doi:10.13735/j.cjdv.1001-7089.202103120]
点击复制

青风藤果浆萃取物对痤疮主要致病菌的体外抑菌活性()
分享到:

《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年09期
页码:
969-974
栏目:
论著
出版日期:
2021-09-01

文章信息/Info

Title:
In Vitro Bacteriostasis of Fruit Pulp Extract of Caulis Sinomenii on the Main Pathogens of Acne
文章编号:
1001-7089(2021)09-0969-06
作者:
廖霞1杨华1刘秋雁1周小云1黄妤1曾茜垚2
1.湖南农业大学生物科学技术学院,湖南 长沙 410128; 2.湖南正清制药集团股份有限公司,湖南 怀化 410000
Author(s):
LIAO Xia1YANG Hua1LIU Qiuyan1ZHOU Xiaoyun1HUANG Yu1ZENG Xiyao2
(1.College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China; 2.Hunan Zhengqing Pharmaceutical Group Co,Ltd.,Huaihua 410000,China)
关键词:
青风藤果浆 乙酸乙酯萃取 痤疮 金黄色葡萄球菌 表皮葡萄球菌 痤疮丙酸杆菌 抗菌活性
Keywords:
Caulis Sinomenii fruit pulp Ethyl acetate extraction Acne Staphylococcus aureus Staphylococcus epidermidis Propionibacterium acnes Antimicrobial activity
分类号:
R 758.73+3
DOI:
10.13735/j.cjdv.1001-7089.202103120
文献标志码:
A
摘要:
目的 探究青风藤果浆萃取物对痤疮致病菌的抑菌活性,为青风藤果浆萃取物治疗痤疮提供实验依据。方法 采集新鲜青风藤成熟期果实制备果浆,应用乙酸乙酯进行萃取,获得青风藤果浆萃取物。通过测定抑菌圈直径、最低抑菌浓度、时间动力学曲线及菌种的生长曲线判断青风藤果浆萃取物的抑菌活性。结果 青风藤果浆萃取物有较好的抑菌活性,对表皮葡萄球菌的抑菌圈直径为(30.86±0.11)mm,最低抑菌浓度为25 mg/mL; 对金黄色葡萄球菌的抑菌圈直径为(21.29±0.59)mm,最低抑菌浓度为35 mg/mL; 对痤疮丙酸杆菌的抑菌圈直径为(30.16±0.88)mm,最低抑菌浓度为25 mg/mL,通过时间动力学曲线和菌种的生长曲线发现青风藤果浆萃取物对痤疮致病菌的生长均有抑制作用。结论 青风藤果浆萃取物对表皮葡萄球菌、金黄色葡萄球菌和痤疮丙酸杆菌均有较好的抑制作用,提示青风藤果浆萃取物对于痤疮临床治疗有潜在价值,可为新型抑菌剂的开发奠定基础。
Abstract:
Objective To explore the antibacterial activity of fruit pulp extract of Caulis Sinomenii on acne pathogens,so as to provide the experimental basis on the treatment of acne by fruit pulp extract of Caulis Sinomenii.Methods Collected fresh mature fruit of Caulis Sinomenii to prepare fruit pulp,and Caulis Sinomenii pulp extract was extracted by ethyl acetate.The antibacterial activity of Caulis Sinomenii pulp extract was measured by the diameter of the inhibition zone,the minimum inhibitory concentration,the time kinetic curve and the growth curve of strains.Results The Caulis Sinomenii pulp extract showed some antibacterial activity based on the following results:The diameter of the inhibition zone against Staphylococcus epidermidis was(30.86±0.11)mm,and the minimum inhibitory concentration was 25 mg/mL.The diameter of the inhibition zone against the Staphylococcus aureus was(21.29±0.59)mm,and the minimum inhibitory concentration was 35 mg/mL.The diameter of the inhibition zone against the bacteriostatic ring for Propionibacterium acnes was(30.16±0.88)mm,and the minimum inhibitory concentration was 25 mg/mL.The resluts of both the time kinetic curve and the growth curve of the strains showed that the fruit pulp extract of Caulis Sinomenii inhibited the growth of pathogenic bacteria of acne.Conclusion Pulp extract of Caulis Sinomenii has a good inhibitory effect on Staphylococcus aureus,Staphylococcus epidermidis and Propionibacterium acnes,suggesting the potential value of Caulis Sinomenii pulp extract in the clinical treatment of acne,which lays a foundation for the development of new bacteriostatic agents.

参考文献/References:

[1] Hafner C,Vogt T.Seborrheic keratosis[J].J Dtsch Dermatol Ges,2008,6(8):664-677.
[2] Jackson J,Alexis A,Berman B,et al.Current understanding ofseborrheic keratosis:prevalence,etiology,clinical presentation,diagnosis,and management[J].J Drugs Dermatol,2015,14(10):1119-1125.
[3] Hafner C,Hartmann A,Real FX,et al.Spectrum of FGFR3 mutations in multiple intraindividual seborrheic keratose[J].J Invest Dermatol,2007,127(8):1883-1885.
[4] Acquaviva J,He S,Zhang C,et al.FGFR3 translo-cations in bladder cancer:differential sensitivity to HSP90 inhibition based on drug metabolism[J].Mol Cancer Res,2014,12(7):1042-1054.
[5] Wollina U.Recent advances in managing and understanding seborrheic keratosis[J].F1000Research,2019,8:1520.
[6] Heidenreich B, Denisova E,Rachakonda S,et al.Genetic alterations in seborrheic keratoses[J].Oncotarget,2017,8(20):36639-36649.
[7] Yamanaka-Takaichi M,Sugawara K,Sumitomo R,et al.The mast cell-SCF-CB1 interaction is a key player in seborrheic keratosis[J].J Histochem Cytochem,2020,68(7):461-471.
[8] Neel VA,Todorova K,Wang J,et al.Sustained aktactivity is required to maintain cell viability in seborrheic keratosis,a benign epithelial tumor[J].J Invest Dermatol,2016,136(3):696-705.
[9] Wilkins HM,Swerdlow RH.Amyloid precursor protein processing and bioenergetics[J].Brain Res Bull,2017,133:71-79.
[10] Chavez-Gutierrez L,Bammens L,Benilova I,et al.The mechanism of gamma-Secretase dysfunction in familial Alzheimer disease[J].EMBO J,2012,31(10):2261-2274.
[11] Tournoy J,Bossuyt X,Snellinx A,et al.Partial loss of presenilins causes seborrheic keratosis and autoimmune disease in mice[J].Hum Mol Genet,2004,13(13):1321-1331.
[12] Minagawa A.Dermoscopy-pathology relationship in seborr-heic keratosis[J].J Dermatol,2017,44(5):518-524.
[13] Karadag AS,Parish LC.The status of the seborrheic keratosis[J].Clin Dermatol,2018,36(2):275-277.
[14] Li Y,Wang Y,Zhang W,et al.Overexpression of amyloid precursor protein promotes the onset of seborrhoeic keratosis and is related to skin ageing[J].Acta Derm Venereol,2018,98(6):594-600.
[15] Liu Y,Beyer A,Aebersold R.On the dependency of cellular protein levels on mRNA abundance[J].Cell,2016,165(3):535-550.
[16] Sogorb-Esteve A,Garcia-Ayllon MS,Llansola M,et al.Inhibition of gamma-secretase leads to an increase in presenilin-1[J].Mol Neurobiol,2018,55(6):5047-5058.
[17] Newman M,Nornes S,Martins RN,et al.Robust homeostasis of Presenilin1 protein levels by transcript regulation[J].Neurosci Lett,2012,519(1):14-19.
[18] Kang DE,Yoon IS,Repetto E,et al.Presenilins mediate phosphatidylinositol 3-kinase/AKT and ERK activation via select signaling receptors.Selectivity of PS2 in platelet-derived growth factor signaling[J].J Biol Chem,2005,280(36):31537-31547.
[19] Guillot F,Kemppainen S,Lavasseur G,et al.Brain-specific basal and novelty-induced alternations in PI3K-Akt and MAPK/ERK signaling in a middle-aged AβPP/PS1 mouse model of alzheimer's disease[J].J Alzheimer's Dis,2016,51(4):1157-1173.

备注/Memo

备注/Memo:
[基金项目] 长沙市科技计划项目(k11907047); 企业合作项目(2021kjc-js008); 湖南省教育厅科学研究项目(20A259); 湖南省自然科学基金项目(2020JJ4043)
[通信作者] 杨华,E-mail:yhua7710@126.com
更新日期/Last Update: 2021-09-01