DONG Liping,MA Jing,XIAO Fengli,et al.Tape Stripping in the Study of Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2021,(03):339-342,356.[doi:10.13735/j.cjdv.1001-7089.202003012]





Tape Stripping in the Study of Atopic Dermatitis
1.安徽医科大学第一附属医院皮肤性病科,安徽医科大学皮肤病研究所,安徽 合肥 230032; 2.安徽医科大学科研实验中心,安徽 合肥230032
DONG Liping1 MA Jing1 XIAO Fengli1 2
(1.Department of dermatology of First Affiliated Hospital, Institute of Dermatology, Anhui Medical University,Hefei 230032 China; 2.The Center for Scientific Research of Anhui Medical University, Hefei 230032,China)
特应性皮炎 胶带剥离法 蛋白质 脂质 细胞因子 RNA
Atopic dermatitis Tape stripping Proteins Lipids Cytokines RNA
R 758.2
特应性皮炎(atopic dermatitis,AD)是一种常见的慢性、复发性、炎症性皮肤病,本病的病因及发病机制尚不明确,屏障缺陷及免疫缺陷在发病中起重要作用。胶带剥离法即通过反复使用合适胶带来依次去除皮肤浅层,由于无创性及可重复性等优点,近年来被应用到AD研究中,发现皮肤浅层中某些物质的RNA、蛋白质、脂质及细胞因子等的异常表达,有助于揭示AD发病机制。本文主要介绍胶带剥离法及其在AD中的具体应用。
Atopic dermatitis(AD)is a common, chronic, recurrent, inflammatory skin disease.Although its etiology and pathogenesis is not clear, skin barrier deficiency and immune deficiency may play an important role in AD pathogenesis.Tape stripping is the repeated use of suitable tape to remove the superficial layers of skin, due to the advantages and repeatability, it has been applied to the study of AD in recent years.By this method, the abnormal expression of certain RNA, proteins, lipids and cytokines has been found in AD.This paper mainly introduces the tape stripping method and its application in AD.


[1] McAleer MA,Jakasa I,Hurault G,et al.Systemic and stratum corneum biomarkers of severity in infant atopic dermatitis include markers of innate and T helper cell-related immunity and angiogenesis[J].Br J Dermatol,2019,180(3):586-596.
[2] Hulshof L,Hack DP,Hasnoe QCJ,et al.A minimally invasive tool to study immune response and skin barrier in children with atopic dermatitis[J].Br J Dermatol,2019,180(3):621-630.
[3] Dyjack N,Goleva E,Rios C, et al.Minimally invasive skin tape strip RNA sequencing identifies novel characteristics of the type 2-high atopic dermatitis disease endotype[J].J Allergy Clin Immunol,2018, 141(4):1298-1309.
[4] Mehul B,Laffet G,Seraidaris A,et al.Noninvasive proteome analysis of psoriatic stratum corneum reflects pathophysi-ological pathways and is useful for drug profiling[J].Br J Dermatol,2017,177(2): 470-488.
[5] Zhao X,Schaffzin JK,Carson J,et al.Analysis of chlorhexidine gluconate in skin using tape stripping and ultrahigh-performance liquid chromatography-tandem mass spectrometry[J].J Pharm Biomed Anal,2020,183:113111.
[6] Dapic I,Kobetic R,Brkljacic L,et al.Quantification of free fatty acids in human stratum corneum using tandem mass spectrometry and surrogate analyte approach[J].Biomed Chromatogr,2018,32(2):e4056.
[7] Clausen ML,Slotved HC,Krogfelt KA,et al.Measurements of AMPs in stratum corneum of atopicdermatitis and healthy skin-tape stripping technique[J].Sci Rep,2018,8(1):1666.
[8] Kim BE,Goleva E,Kim PS,et al.Side-by-side comparison of skin biopsies and skin tape stripping highlights abnormal stratum corneum in atopic dermatitis[J].J Invest Dermatol,2019,139(11): 2387-2389.
[9] Breternitz M,Flach M,Prassler J,et al.Acute barrier disruption by adhesive tapes is influenced by pressure, time and anatomical location: integrity and cohesion assessed by sequential tape stripping.A randomized, controlled study[J].Br J Dermatol,2007, 156(2):231-240.
[10] Solberg J,Ulrich NH,Krustrup D,et al.Skin tape stripping: Which layers of the epidermis are removed?[J].Contact Dermatitis,2019, 80(5):319-321.
[11] Loffler H,Dreher F,Maibach HI.Stratum corneum adhesive tape stripping: influence of anatomical site, application pressure, duration and removal[J].Br J Dermatol,2004,151(4):746-752.
[12] Alsheddi L,Ananthapadmanabhan K,Li SK.Influence of skin furrows on tape stripping in characterizing the depth of skin penetration[J].Int J Pharm,2020,576:118903.
[13] Bashir SJ,Chew AL,Anigbogu A,et al.Physical and physi-ological effects of stratum corneum tape stripping[J].Skin Res Technol,2001, 7(1):40-48.
[14] Tham EH,Dyjack N,Kim BE,et al.Expression and function of the ectopic olfactory receptor OR10G7 in patients with atopic dermatitis[J].J Allergy Clin Immunol,2019,143(5):1838-1848.
[15] Berdyshev E,Goleva E,Bronova I,et al.Lipid abnormalities in atopic skin are driven by type 2 cytokines[J].JCI Insight,2018,3(4): e98006.
[16] Li S,Villarreal M,Stewart S,et al.Altered composition of epidermal lipids correlates with Staphylococcus aureus colo-nization status in atopic dermatitis[J].Br J Dermatol,2017,177(4):e125-e127.
[17] Clausen ML,Slotved HC,Krogfelt KA, et al.Tape stripping technique for stratum corneum protein analysis[J].Sci Rep,2016,6:19918.
[18] Gimalova GF,Karunas AS,Fedorova YY, et al.The study of filaggrin gene mutations and copy number variation in atopic dermatitis patients from Volga-Ural region of Russia[J].Gene,2016,591(1): 85-89.
[19] Clausen ML,Jungersted JM,Andersen PS, et al.Human beta-defensin-2 as a marker for disease severity and skin barrier properties in atopic dermatitis[J].Br J Dermatol,2013,169(3):587-593.
[20] Feingold KR,Elias PM.Role of lipids in the formation and maintenance of the cutaneous permeability barrier[J].Biochim Biophys Acta,2014,1841(3):280-294.
[21] Janssens M,van Smeden J,Gooris GS,et al.Increase in short-chain ceramides correlates with an altered lipid organization and decreased barrier function in atopic eczema patients[J].J Lipid Res, 2012,53(12):2755-2766.
[22] Chiba T,Nakahara T,Kohda F,et al.Measurement of trihydroxy-linoleic acids in stratum corneum by tape-stripping: Possible biomarker of barrier function in atopic dermatitis[J].PLoS One,2019,14(1):e0210013.
[23] Amarbayasgalan T,Takahashi H,Dekio I,et al.Content of vascular endothelial growth factor in stratum corneum well correlates to local severity of acute inflammation in patients with atopic dermatitis[J].Int Arch Allergy Immunol,2012,157(3):251-258.
[24] Yamaguchi J,Aihara M,Kobayashi Y,et al.Quantitative analysis of nerve growth factor(NGF)in the atopic dermatitis and psoriasis horny layer and effect of treatment on NGF in atopic dermatitis[J].J Dermatol Sci,2009,53(1):48-54.
[25] Samochocki Z,Bogaczewicz J,Sysa-Jedrzejowska A, et al.Expression of vascular endothelial growth factor and other cytokines in atopic dermatitis, and correlation with clinical features[J].Int J Dermatol,2016,55(3):e141-146.
[26] Dou YC,Hagstromer L,Emtestam L,et al.Increased nerve growth factor and its receptors in atopic dermatitis: an immunohistochemical study[J].Arch Dermatol Res,2006,298(1):31-37.


 LI Xu ru,CEN Xing hong,BI Tian tian,et al.Effects of Vitamin D Receptor Activators on Atopic Dermatitis Model Mice[J].The Chinese Journal of Dermatovenereology,2015,(03):133.[doi:10.13735/j.cjdv.1001-7089.201408080]
 YAN Yi,CAI Li,CHEN Ting,et al.Comparison of Efficacy of 308 nm Monochromatic Excimer Light and 311 nm Narrowband Ultraviolet for the Treatment of Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2015,(03):251.[doi:10.13735/j.cjdv.1001-7089.201407110]
 HU Hai you,WANG Zhong yong,DONG Da qun,et al.Progree of Epigenetic Mechanisms in Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2015,(03):412.[doi:10.13735/j.cjdv.1001-7089.201404094]
 WANG Juan,WANG Fang yu.The Research Progress of Relationships between Kallikreins and Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2015,(03):524.[doi:10.13735/j.cjdv.1001-7089.201403041]
 LIU Su jun,XU Jian,ZHAO Bin,et al.Application of Labelfree Quantitative Proteomics in Study Differentially Expressed Urinary Proteins in Patients with Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2015,(03):640.[doi:10.13735/j.cjdv.1001-7089.201404102]
 ZHAO Wei,LIU Jun feng,CHEN Da can,et al.Advances of Hapteninduced Murine Model of Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2015,(03):855.[doi:10.13735/j.cjdv.1001-7089.201407156]
 LAN Yuan-ling,YANG Gao yun.Effect of Chemokine like Factor 1 (CKLF1) and CKLF1 Antagonism Peptides C19 on Subsets of Peripheral T Cells of Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2015,(03):1000.[doi:10.13735/j.cjdv.1001.7089.201502096]
 QU Min ye,YUAN Xiao lin,MA Jian.Current Research on Pathogenesis and Mechanism of Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2015,(03):1085.[doi:10.13735/j.cjdv.1001.7089.201411037]
 WANG Min hua,AI Zhi qiong,HUANG Ling,et al.The Prevalence Study of Atopic Dermatitis among Children and Teenagers Aged 3~19 Years in Dali[J].The Chinese Journal of Dermatovenereology,2015,(03):1184.[doi:10.13735/j.cjdv.1001-7089.201412051]


[基金项目] 国家自然科学基金项目(81172838, 81972926); 安徽省自然科学基金项目(1708085MH217); 安徽省学术和科技带头人科研活动经费资助项目(2017D141)
[通信作者] 肖风丽,E-mail: xiaofengli@126.com
更新日期/Last Update: 2021-02-10