[1]刘琪琛,冀永娟,马琳,等.张家口市性接触人群HIV-1遗传多样性与耐药分析[J].中国皮肤性病学杂志,2021,(03):288-292.[doi:10.13735/j.cjdv.1001-7089.202006171]
　LIU Qichen,JI Yongjuan,MA Lin,et al.HIV-1 Genetic Diversity and Drug Resistance in the Population Infected with Sexual Contact in Zhangjiakou[J].The Chinese Journal of Dermatovenereology,2021,(03):288-292.[doi:10.13735/j.cjdv.1001-7089.202006171]
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张家口市性接触人群HIV-1遗传多样性与耐药分析()
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年03期
页码:
288-292
栏目:
性传播疾病
出版日期:
2021-03-01

文章信息/Info

Title:
HIV-1 Genetic Diversity and Drug Resistance in the Population Infected with Sexual Contact in Zhangjiakou
文章编号:
1001-7089(2021)03-0288-05
作者:
刘琪琛1; 冀永娟2; 马琳3; 李岩3; 尹少禹1; 卢志红4; 赵浩文1; 王莹莹3; 路新利3
1.张家口市疾病预防控制中心,河北 张家口 075000; 2.张家口市卫生健康委,河北 张家口 075000; 3.河北省疾病预防控制中心,河北 石家庄 050021; 4.张家口市第五医院,河北 张家口 075000
Author(s):
LIU Qichen1; JI Yongjuan2; MA Lin3; LI Yan3; YIN Shaoyu1; LU Zhihong4; ZHAO Haowen1; WANG Yingying3; LU Xinli3
(1.Zhangjiakou Center for Disease Control and Prevention,Zhangjiakou 075000,China; 2.Health Commission of Zhangjiakou,Zhangjiakou 075000,China; 3.Hebei Provincial Center for Disease Control and Prevention,Shijiazhuang 050021,China; 4.The Fifth Hospital of Zhangjiakou,Zhangjiakou 075000,China)
关键词:
HIV-1;  亚型;  耐药突变;  性接触传播
Keywords:
HIV-1;  Subtype;  Drug resistance mutation;  Sexual contact transmission
分类号:
R 512.91
DOI:
10.13735/j.cjdv.1001-7089.202006171
文献标志码:
B
摘要:
目的 探讨张家口市HIV-1遗传基因多样性和原发耐药基因突变情况。方法 使用In-house方法扩增HIV-1蛋白酶和逆转录酶编码区基因序列,通过系统进化分析和耐药数据库比对确定HIV-1基因型和基因突变情况。结果 张家口市未治疗人群中共有5种HIV-1基因亚型毒株在流行,其中CRF01_AE是最主要流行毒株,占到45.00%(9/20),其次是CRF07_BC(40.00%,8/20)。其他亚型包括B(5.00%,1/20)、CRF68_01B(5.00%,1/20)和CRF65_cpx(5.00%,1/20)。CRF68_01B是河北省首次发现的基因型。HIV-1原发耐药发生率为20.00%(4/20)。没有发现NRTI耐药基因突变。结论 张家口市未接受抗病毒治疗人群中HIV-1遗传基因多样化,耐药基因突变水平较高,成簇传播,加强HIV-1耐药监测和预防控制措施研究已成当务之急。
Abstract:
Objective To investigate HIV-1 genetic diversity and primary drug resistance.Methods In protease and reverse transcriptase gene coding region,HIV-1 gene sequences were amplified using in-house method,and HIV-1 genotypes and drug resistance mutations were analyzed by phylogenetic analysis and comparison of drug resistance database.Results Five genotypes were found in the untreated population in Zhangjiakou,Hebei.Of them,CRF01_AE were the most frequent,accounting for 45.00%(9/20),followed by CRF07_BC(40.00%,8/20).Other genotypes included B(5.00%,1/20),CRF68_01B(5.00%,1/20)and CRF65_cpx(5.00%,1/20).CRF68_01B was first found in Hebei.In this study,a total of four HIV-1 infected people occurred primary resistance mutations,with a total resistance rate of 20.00%(4/20).No NRTI mutation was found.Conclusion The resistance rate among naive HIV-infected persons in Zhangjiakou was higher,and HIV-1 resistant strains were circulating in clusters.Therefore,it was critical for us to strengthen HIV-1 resistance monitoring and prevention.

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更新日期/Last Update: 2021-02-10