[1]王德,刘雪婷,曾丽萍,等.神经肽Y在DNFB诱发的ACD慢性瘙痒模型小鼠背根神经节中的表达[J].中国皮肤性病学杂志,2021,(03):255-260.[doi:10.13735/j.cjdv.1001-7089.201912035]
 WANG De,LIU Xueting,ZENG Liping,et al.Expression of Neuropeptide Y in Dorsal Root Ganglion of Mice with Allergic Contact Dermatitis Induced by DNFB[J].The Chinese Journal of Dermatovenereology,2021,(03):255-260.[doi:10.13735/j.cjdv.1001-7089.201912035]
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神经肽Y在DNFB诱发的ACD慢性瘙痒模型小鼠背根神经节中的表达()
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年03期
页码:
255-260
栏目:
论著
出版日期:
2021-03-01

文章信息/Info

Title:
Expression of Neuropeptide Y in Dorsal Root Ganglion of Mice with Allergic Contact Dermatitis Induced by DNFB
文章编号:
1001-7089(2021)03-0255-06
作者:
王德刘雪婷曾丽萍温玉环李杨阳陶爱林
广州医科大学附属第二医院,广东省过敏反应与重点实验室,呼吸疾病国家重点实验室,广东 广州 510260
Author(s):
WANG DeLIU XuetingZENG LipingWEN YuhuanLI YangyangTAO Ailin
(The Second Affiliated Hospital of Guangzhou Medical University, Guangdong Province Key Laboratory of Allergy & Clinical Immunology, the State Key Laboratory Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China)
关键词:
过敏性接触性皮炎 瘙痒症 神经肽Y(NPY) C57BL/6小鼠 背根神经节 24二硝基氟苯
Keywords:
Allergic contact dermatitis Pruritus Neuropeptide Y(NPY) C57BL/6 mice Dorsal root ganglion 24 dinitrofluorobenzene
分类号:
R 758.22
DOI:
10.13735/j.cjdv.1001-7089.201912035
文献标志码:
A
摘要:
目的 利用2,4二硝基氟苯(DNFB)诱发C57BL/6小鼠建立过敏性接触性慢性瘙痒模型(ACD)。检测神经递质神经肽Y(NPY)等与瘙痒有关的基因在背根神经节(DRG)中的表达,并探讨其对ACD小鼠模型中伴发慢性瘙痒的作用。方法 将8~12周龄的C57BL/6雄性小鼠随机分成两组,分别为DNFB诱导的实验组和单独使用丙酮的对照组。通过观察小鼠的搔抓行为学、分析皮肤的炎症状况、利用HE染色和免疫荧光技术分析皮肤的病理改变和肥大细胞浸润情况、定量PCR分析NPY、MRGPRA3、TRPA1、TRPV1、TRPM8在颈部背根神经节(DRG)的mRNA水平的表达。结果 DNFB诱发的ACD模型组小鼠的搔抓行为显著多于丙酮对照组。同对照组相比,ACD模型组小鼠皮肤表现出明显的炎性皮损,皮损组织表现为表皮增厚,真皮中肥大细胞浸润增加,在颈背部背根神经节中NPY mRNA水平显著增高。结论 DNFB会诱发小鼠产生过敏性接触性慢性瘙痒和加剧炎性皮损的表现,NPY在ACD慢性瘙痒中有重要作用。
Abstract:
Objective To establish an allergic contact dermatitis model(ACD)in C57BL/6 mice induced by 2,4 dinitrofluorobenzene(DNFB)and detect the expression of itch related genes such as neurotransmitter neuropeptide Y(NPY)in dorsal root ganglion(DRG).Methods The C57BL/6 mice,8~12 weeks old, were randomly divided into two groups, a DNFB-induced experiment group and an acetone control group.The behavior of mice and the inflammation of the skin were observed.Skin histology and mast cell infiltration were analyzed by HE staining and immunofluorescence, and quantitative PCR analysis of mRNA expression changes of NPY,MRGPRA3,TRPA1,TRPV1,TRPM8 in the cervical dorsal root ganglion(DRG).Results The spontaneous scratches of ACD model mice was significantly increased than the control group.Compared with the control group, the skin of the ACD model group showed obvious inflammatory skin lesions, skin pathology showed epidermal thickening, mast cell infiltration, and increased NPY mRNA expression level in the cervical and dorsal ganglion.Conclusion DNFB can induce allergic contact chronic itching and promote the promotion of skin inflammation.NPY plays an important role in the ACD chronic itching.

参考文献/References:

[1] Bautista DM, Wilson SR, Hoon MA.Why we scratch an itch: the molecules, cells and circuits of itch[J].Nat Neurosci,2014,17(2):175-182.
[2] Lamotte RH, Dong X, Ringkamp M.Sensory neurons and circuits mediating itch[J].Nat Rev Neurosci,2014,15(1):19-31.
[3] Xu X, Xiao W, Zhang Z, et al.Anti-pruritic and anti-inflammatory effects of oxymatrine in a mouse model of allergic contact dermatitis[J].J Dermatol Sci,2018,91(2):134-141.
[4] Zhao Z, Huo F, Jeffry J, et al.Chronic itch development in sensory neurons requires BRAF signaling pathways[J].J Clin Invest,2013,123(11):4769-4780.
[5] Pall PS, Hurwitz OE, King BA, et al.Psychophysical measurements of itch and nociceptive sensations in an experimental model of allergic contact dermatitis[J].Pain,2015,16(8):741-749.
[6] Sun Y, Chen Z.A gastrin-releasing peptide receptor mediates the itch sensation in the spinal cord[J].Nature,2007,448(7154):700-703.
[7] Qu L, Fan N, Ma C, et al.Enhanced excitability of MRGPRA3- and MRGPRD-positive nociceptors in a model of inflammatory itch and pain[J].Brain,2014,137(4):1039-1050.
[8] Meixiong J, Anderson M, Limjunyawong N, et al.Activation of mast-cell-expressed mas-related G-protein-coupled receptors drives non-histaminergic itch[J].Immunity,2019,50(5):1163-1171.
[9] Feng J, Yang P, Mack MR, et al.Sensory TRP channels contribute differentially to skin inflammation and persistent itch[J].Nat Commun,2017,8(1):980.
[10] Meng J, Moriyama M, Feld M, et al.New mechanism underlying IL-31-induced atopic dermatitis[J].J Allergy Clin Immunol,2018,141(5):1677-1689.
[11] Acton D, Ren X, Di Costanzo S, et al.Spinal neuropeptide Y1 receptor-expressing neurons form an essential excitatory pathway for mechanical itch[J].Cell Reports,2019,28(3):625-639.
[12] Gao T, Ma H, Xu B, et al.The neuropeptide Y system regulates both mechanical and histaminergic itch[J].J Invest Dermatol,2018,138(11):2405-2411.
[13] Pan H, Fatima M, Li A, et al.Identification of a Spinal circuit for mechanical and persistent spontaneous itch[J].Neuron,2019,103(6):1135-1149.

备注/Memo

备注/Memo:
[基金项目] 国家科技重大专项重大课题(2016ZX08011-005); 广州市科技计划项目(201804020042)
[通信作者] 陶爱林,E-mail:taoailin@gzhmu.edu.cn
更新日期/Last Update: 2021-02-10