[1]赵文斌,叶建州,杨雪松,等.傣药咪多领(云南琵琶甲)对增生性瘢痕中表皮干细胞干性及Hedgehog信号通路的影响[J].中国皮肤性病学杂志,2021,(03):246-254.[doi:10.13735/j.cjdv.1001-7089.202008130]
 ZHAO Wenbin,YE Jianzhou,YANG Xuesong,et al.The Influence Mechanism of the Blaps Rynchopetera Fairmaire on the Stemness of Epidermal Stem Cells and Hedgehog Signaling Pathway in Hypertrophic Scar[J].The Chinese Journal of Dermatovenereology,2021,(03):246-254.[doi:10.13735/j.cjdv.1001-7089.202008130]
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傣药咪多领(云南琵琶甲)对增生性瘢痕中表皮干细胞干性及Hedgehog信号通路的影响()
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年03期
页码:
246-254
栏目:
论著
出版日期:
2021-03-01

文章信息/Info

Title:
The Influence Mechanism of the Blaps Rynchopetera Fairmaire on the Stemness of Epidermal Stem Cells and Hedgehog Signaling Pathway in Hypertrophic Scar
文章编号:
1001-7089(2021)03-0246-09
作者:
赵文斌1叶建州1杨雪松1杨恩品1邹映东2罗光云3丛琳1王金容1陆单单1许旭1
1.云南中医药大学第一附属医院皮肤科,云南 昆明 650021; 2.云南中医药大学第一附属医院检验科,云南 昆明 650021; 3.云南中医药大学基础医学院, 云南 昆明 650032
Author(s):
ZHAO Wenbin1 YE Jianzhou1 YANG Xuesong1 YANG Enpin1 ZOU Yingdong2 LUO Guang-yun3 CONG Lin1 WANG Jinrong1 LU Dandan1 XU Xu1
(1.Department of Dermatology,the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming 650021,China; 2.Department of Clinical Laboratory,the First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming 650021,China; 3.School of Basic Medical Sciences,Yunnan University of Traditional Chinese Medicine, Kunming 650032,China)
关键词:
增生性瘢痕 傣药咪多领(云南琵琶甲) 表皮干细胞 干性 Hedgehog信号通路
Keywords:
Hypertrophic scar Blaps rynchopetera Fairmaire Epidermal stem cells Stemness Hedgehog signaling pathway
分类号:
R 751.05
DOI:
10.13735/j.cjdv.1001-7089.202008130
文献标志码:
A
摘要:
目的 探讨傣药咪多领(云南琵琶甲)对增生性瘢痕中表皮干细胞干性及Hedgehog信号通路的影响。方法 构建新西兰大耳兔增生性瘢痕模型,并分离增生性瘢痕组织中表皮干细胞。将动物随机分为对照组、模型组、阳性对照组(PC组,用复方肝素钠尿囊素凝胶干预)及傣药咪多领0.667、1.334、2.668 g/kg组; 表皮干细胞分为对照组,傣药咪多领0.025、0.05、0.1 g/mL组。RT-qPCR和Western blot测各组表皮干细胞标志物(Nanog和Oct4)及Hedgehog信号通路相关基因(SHH、Patch、Smo和Gli-1)mRNA和蛋白的表达水平,进一步通过免疫荧光检测SHH、Patch、Smo和Gli-1的表达水平。CCK-8法检测表皮干细胞增殖活力。流式细胞仪检测各组CD49F、CD29和CK19阳性细胞比率。结果 傣药咪多领对增生性瘢痕具有良好的治疗效果,且显著抑制增生性瘢痕组织中Nanog和Oct4及SHH、Patch、Smo和Gli-1的表达水平,此外,2.668 g/kg剂量傣药咪多领与15 mg/kg剂量复方肝素钠尿囊素凝胶治疗效果相近。傣药咪多领限制抑制表皮干细胞中Nanog和Oct4及SHH、Patch、Smo和Gli-1的表达水平。结论 增生性瘢痕中表皮干细胞干性增强,且Hedgehog信号通路相关蛋白表达上调,而傣药咪多领可逆转这一过程,并对增生性瘢痕具有良好的治疗效果。
Abstract:
Objective To explore the effect of Blaps rynchopetera Fairmaire on the stemness of epidermal stem cells and Hedgehog signaling pathway in hypertrophic scar.Methods Model of hypertrophic scar was constructed, and the epidermal stem cells in the hypertrophic scar tissue were sorted.Animal were divided into control group, model group, positive control group(PC, intervention with compound heparin sodium and allantoin gel)and Blaps rynchopetera Fairmaire 0.667 g/kg, 1.334 g/kg, 2.668 g/kg groups.Epidermal stem cells were divided into control group and Blaps ryhchopetera Fairmaire 0.025 g/mL, 0.05 g/mL, 0.1 g/mL groups.Respectively, RT-qPCR and Western blot were used to detect the expression levels of mRNA and protein of epidermal stem cell markers(Nanog and Oct4)and Hedgehog signaling pathway related genes(SHH, Patch, Smo and Gli-1)in each group.Further, the expression levels of SHH, Patch, Smo and Gli-1 were detected by immunofluorescence.Flow cytometry detected the rate of CD49F, CD29 and CK19 positive cells in each group.Results The Blaps rynchopetera Fairmaire had a good therapeutic effect on hypertrophic scar, and significantly inhibited the expression levels of Nanog, Oct4, SHH, Patch, Smo and Gli-1 in hypertrophic scar tissues.In addition, the therapeutic effect of the Blaps rynchopetera Fairmaire at dose of 2.668 g/kg was similar to that of compound heparin sodium and allantoin gel at a dose of 15 mg/kg.Blaps rynchopetera Fairmaire inhibited the expression levels of Nanog and Oct4, SHH, Patch, Smo and Gli-1 in epidermal stem cells.Conclusion In hypertrophic scars, the stemness of epidermal stem cells was enhanced, and the expression of Hedgehog pathway-related proteins was upregulated.The Blaps rynchopetera Fairmaire reverses this process and had good therapeutic effect on hypertrophic scars.

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备注/Memo

备注/Memo:
[基金项目] 国家自然科学基金项目(81860895)
更新日期/Last Update: 2021-02-10