[1]李巧玲,熊梓汀,杨虹,等.川白芷提取物对痤疮相关致病菌的抑制作用[J].中国皮肤性病学杂志,2021,(01):26-b0d3b509-3788-4837-8800-534f9a4f4451/pdf.[doi:10.13735/j.cjdv.1001-7089.202005029]
　LI Qiaoling,XIONG Ziting,YANG Hong,et al.Antimicrobial Activity of Extract from Angelicae Dahuricae on Acne-associated Bacteria[J].The Chinese Journal of Dermatovenereology,2021,(01):26-b0d3b509-3788-4837-8800-534f9a4f4451/pdf.[doi:10.13735/j.cjdv.1001-7089.202005029]
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川白芷提取物对痤疮相关致病菌的抑制作用
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年01期
页码:
26-b0d3b509-3788-4837-8800-534f9a4f4451/pdf
栏目:
论著
出版日期:
2021-01-01

文章信息/Info

Title:
Antimicrobial Activity of Extract from Angelicae Dahuricae on Acne-associated Bacteria
文章编号:
1001-7089(2021)01-0026-04
作者:
李巧玲; 熊梓汀; 杨虹; 黄蓉; 王寒冰; 罗超; 刘剑
成都中医药大学医学与生命科学学院,四川成都610031
Author(s):
LI Qiaoling; XIONG Ziting; YANG Hong; HUANG Rong; WANG Hanbing; LUO Chao; LIU Jian
Department of Medicine and Life Science,Chengdu University of TCM,Chengdu 610031,China
关键词:
白芷;  乙醇提取物;  痤疮;  痤疮丙酸杆菌;  表皮葡萄球菌;  金黄色葡萄球菌
Keywords:
Angelicae dahuricae;  Ethanol extract;  Acne;  Propionibacterium acnes;  Staphylococcus epidermidis;  Staphylococcus aureus
分类号:
R 758.73+3
DOI:
10.13735/j.cjdv.1001-7089.202005029
文献标志码:
A
摘要:
目的 探讨川白芷的乙醇提取物对痤疮的主要相关致病茵(痤疮丙酸杆菌、表皮葡萄球菌和金黄色葡萄球菌)的抑制作用。方法 首先用生长抑制试验确定川白芷提取物对痤疮相关致病菌是否有抑制作用,然后通过微量液体稀释法和平板培养基分别测定川白芷提取物对痤疮丙酸杆菌、表皮葡萄球菌和金黄色葡萄球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),以此评价川白芷提取物对痤疮相关致病菌的抑制作用。结果 川白芷提取物对痤疮丙酸杆菌ATCC6919的MIC和MBC分别为50 mg/mL和100 mg/mL,阳性对照克林霉素的MIC和MBC为0.098 μg/mL和0.195 μg/mL。川白芷提取物对表皮葡萄球菌CMCC(B)26069的MIC和MBC分别为50 mg/mL和100 mg/mL,阳性对照三氯生的MIC和MBC为6.25 μg/mL和12.5 μg/mL。川白芷提取物对金黄色葡萄球菌ATCC25923的MIC和MBC分别为12.5 mg/mL和25 mg/mL,阳性对照红霉素的MIC和MBC为0.065 μg/mL和0.195 μg/mL。三组试验的抑菌环直径与川白芷提取物的浓度相关(r=1.00,P<0.01)。结论 川白芷提取物在体外对痤疮相关致病菌(痤疮丙酸杆菌、表皮葡萄球菌和金黄色葡萄球菌)具有一定的抑制作用,此抑制作用与浓度有关。
Abstract:
Objective To investigate the antimicrobial activity of ethanol extract from Angelicae dahuricae on acne-associated bacteria,including Propionibacterium acnes,Staphylococcus epidermidis and Staphylococcus aureus.Methods The antimicrobial effect of ethanol extract on the bacterium was observed by growth inhibition test(agar dilution method).The minimum inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)of the extract to Propionibacterium acnes,Staphylococcus epidermidis and Staphylococcus aureus were evaluated by broth microdilution method and agar plate.Results The MIC and MBC of ethanol extract from Angelicae dahuricae to Propionibaeterium acnes ATCC6919 were 50 mg/mL and 100 mg/mL.The MIC and MBC of clindamycin to Propionibacterium acnes ATCC6919 were 0.098 μg/mL and 0.195 μg/mL respectively.The MIC and MBC of this extract to Staphylococcus epidermidis CMCC(B)26069 were 50 mg/mL and 100 mg/mL. The MIC and MBC of triclosan as the positive control were 6.25 μg/mL and 12.5 μg/mL. The MIC and MBC of this extract to Staphylococcus aureus ATCC25923 were 12.5 mg/mL and 25 mg/mL.The MIC and MBC of erythromycin were 0.098 μg/mL and 0.195 μg/mL.The diameter of bacteriostatic ring in test group was correlated with the concentration of extract from Angelica dahuricae(r=1.00,P<0.01). Conclusion The ethanol extract from Angelicae dahuricae has a certain inhibitory effect on acne-associated bacterium in vitro,and this inhibitory effect is related to its concentration.

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备注/Memo

备注/Memo:
[基金项目] 成都中医药大学杏林学者学科人才科研提升计划基金(ZRQN2018003) [作者单位] 成都中医药大学医学与生命科学学院,四川 成都 610031
更新日期/Last Update: 2021-01-10