[1]刘力豪,练霓,崔盘根,等.寻常性银屑病表皮HLA-G甲基化水平的检测[J].中国皮肤性病学杂志,2020,(11):1228-1232.[doi:10.13735/j.cjdv.1001-7089.202005088]
 LIU Lihao,LIAN Ni,CUI Pangen,et al.Investigation of the Relationship between Promoter Methylation of Human Leukocyte Antigen(HLA)-G and Psoriasis[J].The Chinese Journal of Dermatovenereology,2020,(11):1228-1232.[doi:10.13735/j.cjdv.1001-7089.202005088]
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寻常性银屑病表皮HLA-G甲基化水平的检测()
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年11期
页码:
1228-1232
栏目:
论著
出版日期:
2020-11-01

文章信息/Info

Title:
Investigation of the Relationship between Promoter Methylation of Human Leukocyte Antigen(HLA)-G and Psoriasis
文章编号:
1001-7089(2020)11-1228-05
作者:
刘力豪1练霓1崔盘根1蒋明军2郝志敏1陈敏1
1.中国医学科学院 北京协和医学院皮肤病医院,江苏 南京 210042; 2.中国医学科学院 北京协和医学院 皮肤病研究所中心实验室,江苏 南京 210042
Author(s):
LIU Lihao1LIAN Ni1CUI Pangen1JIANG Mingjun2HAO Zhimin1CHEN Min1
1.Institute of Dermatology and Hospital of Skin Diseases CAMS&PUMC,Nanjing 210042,China; 2.Central Laboratory,Institute of Dermatology and Hospital of Skin Diseases CAMS&PUMC,Nanjing 210042,China
关键词:
银屑病 DNA甲基化 人类白细胞抗原G
Keywords:
Psoriasis DNA methylation Human leukocyte antigen G
分类号:
R 758.63
DOI:
10.13735/j.cjdv.1001-7089.202005088
文献标志码:
A
摘要:
目的 通过检测寻常性银屑病患者表皮人类白细胞抗原-G(human leukocyte antigen-G,HLA-G)基因启动子区甲基化水平,研究HLA-G基因启动子区甲基化对银屑病发生发展的影响。方法 采用亚硫酸氢钠测序技术检测HLA-G基因启动子区甲基化水平,将银屑病患者皮损部位、非皮损部位及健康正常人表皮进行对照,分析其与病情严重程度的相关性。以银屑病皮损面积及严重程度指数(PASI)评估银屑病病情严重程度。结果 在56例银屑病患者皮损、非皮损部位及28名健康对照者表皮中检测到不同程度的HLA-G基因启动子区甲基化,分别为(36.69±7.92)%、(37.38±6.90)%和(57.23±11.89)%,银屑病患者皮损及非皮损部位HLA-G基因启动子区甲基化水平均显著低于健康正常人(P<0.001),皮损及非皮损部位HLA-G启动子区甲基化水平比较差异无统计学意义(P>0.05)。皮损部位HLA-G启动子区甲基化水平与银屑病的严重程度不相关(r=-0.24,P>0.05)。结论 银屑病患者表皮中HLA-G基因启动子区甲基化水平明显降低,可能是银屑病患者的一种表观遗传标志。
Abstract:
Objective To investigate the role of promoter methylation of human leukocyte antigen(HLA)-G in the development of psoriasis by detecting the promoter methylation levels of HLA-G in the psoriatic epidermis in patients with psoriasis vulgaris.Methods Sodium Bisulfite Sequencing was applied to detect the promoter methylation levels of HLA-G in psoriatic lesions and the non-lesional areas from 56 patients with psoriasis vulgaris,and normal epidermis from 28 healthy controls.We compared and evaluated the correlation between the promoter methylation levels of HLA-G and the severity of the disease.The severity of psoriasis was assessed by PASI.Results Different degrees of promoter methylation levels of HLA-G were detected in the lesional and non-lesional epidermis of 56 patients with psoriasis vulgaris and 28 healthy controls,at(36.69±7.92)%,(37.38±6.90)%,(57.23±11.89)%,respectively.The promoter methylation levels of HLA-G in both lesional and non-lesional epidermis from the patients with psoriasis were significantly lower than healthy controls(P<0.001).There is no significant difference for HLA-G methylation levels between lesional and non-lesional epidermis from the patients with psoriasis(P>0.05).However,the methylation levels of HLA-G lesional and non-lesional epidermis from the patients with psoriasis are not related to the severity of psoriasis(P>0.05).Conclusion The promoter methylation levels of HLA-G in psoriatic epidermal lesions were significantly decreased,suggesting that promoter methylation levels of HLA-G could be an epigenetic marker in psoriasis.

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备注/Memo

备注/Memo:
[通信作者] 陈敏,E-mail:drchenmin821@126.com
更新日期/Last Update: 2020-11-16