[1]许莉,陈娜.淋球菌经NLRP3途径调节小鼠脾细胞Th17/Treg平衡[J].中国皮肤性病学杂志,2020,(10):1116-1120.[doi:10.13735/j.cjdv.1001-7089.202001083]
 XU Li,CHEN Na.Neisseria Gonorrhoeae Regulates Th17/Treg Balance in Murine Splenocytes Via NLRP3 Pathway[J].The Chinese Journal of Dermatovenereology,2020,(10):1116-1120.[doi:10.13735/j.cjdv.1001-7089.202001083]
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淋球菌经NLRP3途径调节小鼠脾细胞Th17/Treg平衡()
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年10期
页码:
1116-1120
栏目:
论著
出版日期:
2020-10-01

文章信息/Info

Title:
Neisseria Gonorrhoeae Regulates Th17/Treg Balance in Murine Splenocytes Via NLRP3 Pathway
文章编号:
1001-7089(2020)10-1116-05
作者:
许莉陈娜
武汉市第一医院皮肤科,湖北 武汉 430022
Author(s):
XU LiCHEN Na
Department of Dermatology,Wuhan No.1 Hospital,Wuhan 430022,China
关键词:
淋球菌 NLRP3 Th17/Treg
Keywords:
Neisseria gonorrhoeae NLRP3 Th17/Treg
分类号:
R 759.2
DOI:
10.13735/j.cjdv.1001-7089.202001083
文献标志码:
A
摘要:
目的 研究NLRP3对小鼠脾细胞淋球菌感染模型中Th17/Treg平衡的调节作用。方法 体外分离、培养小鼠脾细胞后,分为如下4组:空白对照组、NLRP3拮抗剂(MCC950)组、淋球菌感染组、淋球菌+MCC950组。培养48 h后收集细胞,采用细胞内染色流式细胞术检测Th17/Treg细胞比例,利用实时荧光定量PCR检测NLRP3、Th17/Treg特异性转录因子RORγt、Foxp3 mRNA表达水平,Western blot检测NLRP3、RORγt、Foxp3蛋白表达。ELISA法检测脾细胞培养上清中Th17/Treg相关细胞因子IL-17、TGF-β、IL-10水平。结果 脾细胞在淋球菌的刺激下,Th17细胞和Treg细胞比例增多,细胞内NLRP3、Th17/Treg特异性转录因子RORγt、Foxp3 mRNA和蛋白表达水平升高,细胞培养上清中IL-17、TGF-β、IL-10含量增高(P<0.01); 加入MCC950预处理后,Th17细胞比例、细胞内NLRP3、RORγt mRNA和蛋白表达、细胞培养上清中IL-17含量明显降低,Treg细胞比例、细胞内Foxp3 mRNA和蛋白表达、细胞培养上清中TGF-β、IL-10含量明显增高,与淋球菌感染组比较差异有统计学意义(P<0.01)。结论 淋球菌通过NLRP3途径诱导小鼠脾细胞向Th17细胞分化,调节Th17/Treg细胞平衡。
Abstract:
Objective To investigate the regulatory effect of NLRP3 on the balance of Th17/Treg in murine splenocytes infected with Neisseria gonorrhoeae.Methods Murine splenocytes were isolated and cultured in vitro,and divided into four groups:control group,NLRP3 antagonist(MCC950)group,Neisseria gonorrhoeae group,Neisseria gonorrhoeae+MCC950 group.The splenocytes were collected after 48 hours of culture,the proportions of Th17/Treg cells were detected by intracellular staining using flow cytometry.The mRNA expressions of NLRP3,Th17/Treg-specific transcription factors RORγt,Foxp3 were detected by real-time PCR.The protein expression levels of NLRP3,RORγt,Foxp3 were determined by Western blot.The concentrations of Th17/Treg related cytokines IL-17,TGF-β,IL-10 in the supernatant were detected by ELISA.Results Under the stimulation of Neisseria gonorrhoeae,the proportions of Th17 cells and Treg cells,the mRNA and protein levels of NLRP3,Th17/Treg-specific transcription factors RORγt,Foxp3 in the splenocytes and the concentrations of IL-17,TGF-β,IL-10 in the supernatant were increased than those in the controls(all P<0.01).After pretreated with MCC950,the proportion of Th17 cells,the mRNA and protein levels of NLRP3,RORγt in the splenocytes and the concentration of IL-17 in the supernatant were significantly lower than those in Neisseria gonorrhoeae group.The proportion of Treg cells,the mRNA and protein levels of Foxp3 in the splenocytes and the concentrations of TGF-β,IL-10 in the supernatant were significantly higher than those in Neisseria gonorrhoeae group(all P<0.01).Conclusion Neisseria gonorrhoeae induces the differentiation of Th17 cells in murine splenocytes via NLRP3 pathway,and regulates the balance of Th17/Treg cells.

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备注/Memo

备注/Memo:
[基金项目] 武汉市卫生健康委科研项目(WX15B13,WX19D28)
更新日期/Last Update: 2020-10-15