[1]李程彬,陈佳,李婷,等.DANCR通过激活YAP促进黑素瘤细胞侵袭并与不良预后的相关性[J].中国皮肤性病学杂志,2020,(10):1104-1109.[doi:10.13735/j.cjdv.1001-7089.201912047]
 LI Chengbin,CHEN Jia,LI Ting,et al.DANCR Promotes Melanoma Cell Invasion Via Activating YAP and Correlationship with Patients' Poor Prognosis[J].The Chinese Journal of Dermatovenereology,2020,(10):1104-1109.[doi:10.13735/j.cjdv.1001-7089.201912047]
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DANCR通过激活YAP促进黑素瘤细胞侵袭并与不良预后的相关性()
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年10期
页码:
1104-1109
栏目:
论著
出版日期:
2020-10-01

文章信息/Info

Title:
DANCR Promotes Melanoma Cell Invasion Via Activating YAP and Correlationship with Patients' Poor Prognosis
文章编号:
1001-7089(2020)10-1104-06
作者:
李程彬陈佳李婷徐威龙贾晶
西安交通大学第一附属医院整形美容?颌面外科,陕西 西安 710061
Author(s):
LI ChengbinCHEN JiaLI TingXU WeilongJIA Jing
Department of Plastic,Cosmetic and Maxilofacial Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China
关键词:
长链非编码RNA DANCR 黑素瘤 侵袭 Yes相关蛋白(YAP) 基质金属蛋白酶2 基质金属蛋白酶9
Keywords:
LncRNA DANCR Melanoma Invasion YAP MMP2 MMP9
分类号:
R 739.5
DOI:
10.13735/j.cjdv.1001-7089.201912047
文献标志码:
A
摘要:
目的 根据本院收集的黑素瘤组织标本分析长链非编码RNA DANCR(long non-coding RNA differentiation antagonizing non-protein coding RNA,LncRNA DANCR)与黑素瘤临床预后关系。阐明DANCR在黑素瘤侵袭中所扮演的角色。方法 用激光捕获法获取黑素瘤组织标本以分析DANCR表达量与黑素瘤患者临床进展的关系; 用A375和B16细胞株行体外实验,以慢病毒为载体在细胞中敲低DANCR。基质胶覆盖的上层小室用于Transwell模型检测细胞侵袭能力; 通过Western blot实验和ELISA检测MMP2、MMP9在细胞中表达水平和细胞外分泌水平。通过Western blot实验检测p-YAP和YAP表达以明确其活性; 用XMU-MP-1和Verteporfin(VP)分别在黑素瘤细胞株中激活和抑制YAP活性。结果 临床数据分析可得,DANCR表达量与临床M分期和病理N分期、Breslow深度和Clark值均密切相关。在A375和B16细胞中敲低DANCR可抑制细胞侵袭,并下调MMP2、MMP9表达和细胞外分泌(P<0.05)。敲低DANCR下调p-YAP蛋白和激活YAP,而激活YAP同样可促进A375和B16细胞侵袭(P<0.05)和MMP2、MMP9表达。同样,VP(5 μmol/L)处理细胞24 h抑制YAP可逆转DANCR过表达所诱导的细胞侵袭和MMP2、MMP9表达上调。结论 DANCR与黑素瘤不良预后相关,敲低DANCR可通过下调YAP活性抑制细胞侵袭。
Abstract:
Objective To analyze the association of LncRNA DANCR expression in melanoma tissues and clinical prognosis.Clarifying the role of DANCR in melanoma cell invasion.Methods Using Laser capture microdection to analyze the relation between DANCR expression and melanoma clinical progression.Lentivirus carrying short hairpin shRNA targeting DANCR were used to transfected into A375 and B16 melanoma cell lines.Transwell assay was used to detect cell invasion in vitro.Used Western blot and ELISA to detect the expression levels of MMP2 and MMP9.The expression of YAP and p-YAP was detected by Western blot.XMU-MP-1 and Verteporfin were used to activate and inhibite YAP activity respectively.Results Clinical analysis demonstrated the DANCR expression is closely related with M stage,pathological N stage,Breslow depth value and Clark value.Knocking down DANCR in A375 and B16 cells can inhibit cell invasion and down-regulate MMP2,MMP9 expression and extracellular secretion(P<0.05).DANCR knockdown decreased p-YAP and activated YAP,and activating with VP(5 μmol/L)for 24 h YAP could also promote A375 and B16 cell invasion and MMP2,MMP9 expression.Similarly,inhibiting YAP could reverse cell invasion and upregulation of MMP2,MMP9 that induced by DANCR overexpression.Conclusion DANCR was related to poor prognosis of melanoma,and knocking DANCR down could repress cell invasion through down-regulating YAP.

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备注/Memo

备注/Memo:
[基金项目] 陕西省重点研发计划一般项目(2018SF-250)
[通信作者]贾晶,E-mail:jiayu0717@126.com
更新日期/Last Update: 2020-10-15