[1]马委委,王洁,殷旭峰,等.miR-769-5p在急性中长波紫外线旁观者效应中的作用[J].中国皮肤性病学杂志,2020,(07):748-753.[doi:10.13735/j.cjdv.1001-7089.201908167]
　MA Weiwei,WANG Jie,YIN Xufeng,et al.The Role of miR-769-5p in the Acute UVA/UVB-radiation Induced Bystander Effect[J].The Chinese Journal of Dermatovenereology,2020,(07):748-753.[doi:10.13735/j.cjdv.1001-7089.201908167]
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miR-769-5p在急性中长波紫外线旁观者效应中的作用
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年07期
页码:
748-753
栏目:
论著
出版日期:
2020-07-01

文章信息/Info

Title:
The Role of miR-769-5p in the Acute UVA/UVB-radiation Induced Bystander Effect
文章编号:
1001-7089(2020)07-0748-06
作者:
马委委; 王洁; 殷旭峰; Lorna Martin Kasyanju Carrero; 周炳荣
南京医科大学第一附属医院皮肤科,江苏 南京 210029
Author(s):
MA Weiwei; WANG Jie; YIN Xufeng; Lorna Martin Kasyanju Carrero; ZHOU Bingrong
(Department of Dermatology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)
关键词:
紫外线辐射诱导的旁观者效应;  人真皮成纤维细胞;  miR-769-5p;  增殖;  氧化损伤;  凋亡
Keywords:
UV-radiation induced bystander effect;  Human dermal fibroblasts;  miR-769-5p;  Proliferation;  Oxidative damage;  Apoptosis
分类号:
R 751
DOI:
10.13735/j.cjdv.1001-7089.201908167
文献标志码:
A
摘要:
目的 探讨miR-769-5p在急性中长波紫外线旁观者效应中的作用。方法 用miR-769-5p mimics转染人真皮成纤维细胞,培养24 h后取培养上清孵育旁观者细胞,培养48 h后检测细胞增殖率、凋亡率及氧化损伤水平。预先用miR-769-5p inhibitor下调细胞中miR-769-5p的表达,24 h后与辐射条件培养基共孵育,48 h后检测细胞的增殖率、凋亡率及氧化损伤水平。结果 miR-769-5p mimics可上调细胞中miR-769-5p的表达,过表达的miR-769-5p可通过上清在细胞间转移,且旁观者细胞的增殖水平明显降低,凋亡率和氧化损伤水平明显升高。使用miR-769-5p inhibitor下调细胞中miR-769-5p的表达,可以观察到急性中长波紫外线旁观者效应的减弱,即接受辐射条件培养基共孵育后,inhibitor预处理组的增殖水平明显高于对照组,凋亡率和氧化损伤水平明显降低。结论 过表达的miR-769-5p能够被旁观者细胞摄取,并介导紫外线旁观者效应。
Abstract:
Objective To investigate the role of miR-769-5p in acute UVA/UVB-radiation induced bystander effect.Methods Human dermal fibroblasts were transfected with miR-769-5p mimics, and 24 h later bystander cells were cultured supernatants obtained.After 48 h of culture,the cell proliferation rate,apoptosis rate and oxidative damage were detected.The expression of miR-769-5p was down-regulated by transfection of miR-769-5p inhibitor.After 24 h,it was incubated with radiation-conditioned medium.After 48 h,the biological indicators as above were detected.Results miR-769-5p mimics up-regulated the expression of miR-769-5p in cells.Overexpression of miR-769-5p could transfer between cells,and the proliferation of bystander cells was significantly decreased.Apoptosis rate and the level of oxidative damage were significantly increased.Using miR-769-5p inhibitor to down-regulate the expression of miR-769-5p in advance could offset the UVA/UVB-radiation induced bystander effects to some extent including increasing proliferation level,reducing apoptotic rate and oxidative damage level.Conclusion Overexpressed miR-769-5p can be uptaken by bystander cells and mediate the UV-radiation induced bystander effects.

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备注/Memo

备注/Memo:
[收稿日期] 2019- 09- 04[修回日期] 2020- 01- 15
[基金项目] 国家自然科学基金(81573072)
[通信作者] 周炳荣,E-mail:bingrong.2002@163.com
[Corresponding author] ZHOU Bingrong,E-mail: bingrong.2002@163.com
更新日期/Last Update: 2020-07-01