[1]裴悦,肖水明,莫秀梅,等.培土清心方对特应性皮炎儿童肠道菌群的影响[J].中国皮肤性病学杂志,2020,(06):688-691.[doi:10.13735/j.cjdv.1001-7089.201909161]
 PEI Yue,XIAO Shuiming,MO Xiumei,et al.Effects of PTQX Formulae on Gut Microbiota in Children with Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2020,(06):688-691.[doi:10.13735/j.cjdv.1001-7089.201909161]
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培土清心方对特应性皮炎儿童肠道菌群的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年06期
页码:
688-691
栏目:
中医中药
出版日期:
2020-06-01

文章信息/Info

Title:
Effects of PTQX Formulae on Gut Microbiota in Children with Atopic Dermatitis
文章编号:
1001-7089(2020)06-0688-04
作者:
裴悦1肖水明2莫秀梅1刘俊峰1梁家芬1张瑜1王楚阳1黄志海1李红毅1
1.广州中医药大学第二附属医院皮肤科,广东 广州 510120; 2.中国中医科学院中药研究所,北京 100700
Author(s):
PEI Yue1XIAO Shuiming2MO Xiumei1LIU Junfeng1LIANG Jiafen1ZHANG Yu1WANG Chu-yang1HUANG Zhihai1LI Hongyi1
(1.Department of Dermatology,the Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510120,China; 2.Institute of Chinese Meteria Medica China Academy of Chinese Medical Sciences,Beijing 100700,China)
关键词:
特应性皮炎 肠道菌群 培土清心方 16S rRNA基因测序检测
Keywords:
Atopic dermatitis Gut microbiota PTQX formulae 16S rRNA tests
分类号:
R 758.2
DOI:
10.13735/j.cjdv.1001-7089.201909161
文献标志码:
B
摘要:
目的 通过高通量测序手段检测比较特应性皮炎(AD)患儿与健康对照儿童肠道菌群结构差异,并对比培土清心方干预前后对AD患儿的临床疗效及肠道菌群的改变情况,初步探索培土清心方对肠道菌群的影响。方法 共纳入60例受试者(实验组、健康对照组各30例),实验组分别在口服培土清心方0周(AD.0W)、4周(AD.4W)进行SCORAD评分并采集粪便样品,对照组在0周时采集粪便样品,统一对粪便样品进行16S rRNA基因测序检测。结果 实验组用药4周(AD.4W)SCORD评分降低,差异有统计学意义(P<0.05)。肠道菌群测序结果:①Alpha多样性:AD.0W 组>AD.4W 组>健康对照组(P>0.05)。②差异菌门水平:Firmicutes(厚壁菌门)相对丰度:AD.0W组<健康对照组<AD.4W组(P>0.05); Bacteroidetes(拟杆菌门)相对丰度:AD.4W组<健康对照组<AD.0W组(P>0.05); Faecalibacterium(柔嫩梭菌属)相对丰度:AD.0W组<AD.4W组<健康对照组,差异有统计学意义(P=0.00<0.05)。结论 特应性皮炎儿童和健康儿童肠道菌群多样性方面无差异,柔嫩梭菌属为健康人优势菌属; 培土清心方可改善AD患儿病情,对肠道菌群多样性无明显影响,其可能通过上调厚壁菌门、柔嫩梭菌属,下调拟杆菌门丰度来达到减轻肠道炎症、改善AD的作用。
Abstract:
Objective To detect and compare the structural difference of gut microbiota between children with atopic dermatitis(AD)and healthy children by high throughput sequencing,and to compare the changes in the clinical efficacy and gut microbiota of children with AD before and after the intervention of Peitu Qingxin(PTQX)formulae,in order to preliminarily explore the effect of PTQX formulae on intestinal flora.Methods A total of 60 subjects were included(experimental group,healthy control group,with 30 cases in each group).The experimental group was given oral PTQX formula for SCORAD scoring and stool samples were collected at 0 weeks(AD.0W)and 4 weeks(AD.4W),respectively.In the control group,stool samples were collected at 0 weeks for 16S rRNA tests.Results In the experimental group,the score of SCORD was decreased at 4 weeks(AD.4W),and the difference was statistically significant(P<0.05).Gut microbiota sequencing results were as follows:①Alpha diversity:AD.0W group > AD.4W group > healthy control group(P>0.05).② The relative abundance of differential bacteria:Firmicutes:AD.0W group < healthy control group < AD.4W group(P>0.05); Bacteroidetes:AD.4W group < healthy control group < AD.0W group); Faecalibacterium:AD.0W group<AD.4W group < healthy control group,and the difference was statistically significant(P=0.00<0.05).Conclusion There is no difference in gut microbiota diversity between children with atopic dermatitis and healthy children.Faecalibacterium is the dominant genus in healthy people.PTQX formulae improves the condition of children with AD and has no significant effect on gut microbiota diversity.It may reduce intestinal inflammation and improve AD by up-regulating the abundance of Firmicutes,Faecalibacterium and down-regulating the abundance of Bacteroidetes.

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备注/Memo

备注/Memo:
[基金项目] 广东省中医院中医药科技技术研究专项(YN2016ML08); 国家自然科学基金(81774307); 广东省中医药局科研项目(20182042); 国家中医药管理局中医学术流派传承工作室建设项目-岭南皮肤病流派传承工作室(LP0119045),国家中医药管理局禤国维国医大师传承工作室
[通信作者] 李红毅,E-mail:lihongyich@126.com
[Corresponding author]LI Hongyi,E-mail:lihongyich@126.com
更新日期/Last Update: 2020-06-05