[1]史云容,张峻岭,康元.葡萄籽提取物原花青素对UVA照射HaCaT细胞表达AQP3、Caspase-14和BH mRNA的影响[J].中国皮肤性病学杂志,2020,(06):640-643.[doi:10.13735/j.cjdv.1001-7089.201908119]
 SHI Yunrong,ZHANG Junling,KANG Yuan.Effects of Grape Seed Proanthocyanin Extract on the Expression of AQP3,Caspase-14 and BH mRNA in UVA Irradiated HaCaT Cells[J].The Chinese Journal of Dermatovenereology,2020,(06):640-643.[doi:10.13735/j.cjdv.1001-7089.201908119]
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葡萄籽提取物原花青素对UVA照射HaCaT细胞表达AQP3、Caspase-14和BH mRNA的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年06期
页码:
640-643
栏目:
论著
出版日期:
2020-06-01

文章信息/Info

Title:
Effects of Grape Seed Proanthocyanin Extract on the Expression of AQP3,Caspase-14 and BH mRNA in UVA Irradiated HaCaT Cells
文章编号:
1001-7089(2020)06-0640-04
作者:
史云容1 张峻岭2康元3
1.山东省济宁市第一人民医院,山东 济宁 272000; 2.天津市中医药研究院附属医院,天津 300120; 3.天津中医药大学第一附属医院,天津 300120
Author(s):
SHI Yunrong1ZHANG Junling2KANG Yuan3
(1.Jining No.1 People's Hospital,Jining 272000,China; 2.Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,Tianjin 300120,China; 3.First Teaching Hospital of Tianjin University of TCM,Tianjin 300120,China)
关键词:
皮肤屏障 葡萄籽提取物原花青素 长波紫外线 HaCaT细胞 水通道蛋白3 半胱氨酸蛋白酶14 博来霉素水解酶
Keywords:
Skin barrier Grape seed proanth ocyanidin extract(GSPE) Long-wave UVA HaCaT cells AQP3 Caspase-14 BH
分类号:
R 751
DOI:
10.13735/j.cjdv.1001-7089.201908119
文献标志码:
A
摘要:
目的 通过研究UVA照射前后及不同浓度葡萄籽提取物原花青素(grape-seed proanthocyanins extract,GSPE)干预的HaCaT细胞表达水通道蛋白3(AQP3)、半胱氨酸蛋白酶14(Caspase-14)和博来霉素水解酶(BH)mRNA的变化,探索GSPE是否能够修复长波紫外线引起的皮肤屏障损伤。方法 实验分为五个组:空白对照组、单纯光照组(选取UVA的能量密度为25 J/cm2)、照光联合不同浓度GSPE干预组(10、50、100 μg/mL),采用RT-PCR法分别检测各组AQP3、Caspase-14和BH mRNA的表达量。结果 25 J/cm2 的UVA可以提高AQP3 mRNA表达量。GSPE药物浓度大于10 μg/mL时,随药物浓度增加,可以逐渐降低光照后高表达的基因水平,并呈浓度依赖性。UVA可下调BH和Caspase-14 mRNA表达量,而GSPE可以提高光照后降低的基因表达量,且呈浓度依赖性,逐渐使其恢复正常。结论 合适浓度的GSPE可以调节UVA对HaCaT细胞AQP3、Caspase-14和BH mRNA表达的影响,并使其趋于正常,推测其具有修复长波紫外线引起的皮肤屏障功能损伤的作用。
Abstract:
Objective To investigate whether the grape seed proanthocyanin extract(GSPE)plays a certain role in repairing the damage to skin barrier caused by long-wave UV by detecting mRNA expressions of AQP3,Caspase-14 and BH in the HaCaT cells induced by UVA irradiation and different GSPE concentrations.Methods The experiment was divided into five groups:blank control group,simple light exposure group(the energy density of UVA was 25 J/cm2),light exposure combined with different concentrations of GSPE intervention groups(10,50,100 μg/mL).RT-PCR was used to detect the expression of AQP3,Caspase-14 and BH mRNA in each group.Results UVA(25 J/cm2)could increase the expression of AQP3 mRNA.When the concentration of GSPE was more than 10 μg/mL,with the increase of the concentration of GSPE,the overexpression level could be decreased gradually in a concentration-dependent manner.UVA could down-regulate the expression of BH and Caspase-14 mRNA.By contrast,the expression could be increased gradually to normal level by GSPE at different concentrations in a dose-dependent manner.Conclusion Appropriate concentration of GSPE can regulate the effect of UVA on the expression of AQP3,Caspase-14 and BH mRNA in HaCaT cells,and help them return to normal.It is speculated that GSPE can repair the damage to skin barrier function caused by UVA.

参考文献/References:

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备注/Memo

备注/Memo:
[通信作者] 张峻岭,E-mail:zhangjunlingtj@126.com
[Corresponding author]ZHANG Junling,E-mail:zhangjunlingtj@126.com
更新日期/Last Update: 2020-06-05