[1]韩晓凤,徐伟,昌丽,等.毫火针激活Wnt/β-catenin信号诱导小鼠毛囊黑素干细胞的增殖分化[J].中国皮肤性病学杂志,2020,(06):634-639.[doi:10.13735/j.cjdv.1001-7089.201909068]
 HAN Xiaofeng,XU Wei,CHANG Li,et al.Fire-acupuncture Induces Proliferation and Differentiation of Melanocyte Stem Cells in Mouse Hair Follicle by Activating Wnt/β-catenin Signal[J].The Chinese Journal of Dermatovenereology,2020,(06):634-639.[doi:10.13735/j.cjdv.1001-7089.201909068]
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毫火针激活Wnt/β-catenin信号诱导小鼠毛囊黑素干细胞的增殖分化
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年06期
页码:
634-639
栏目:
论著
出版日期:
2020-06-01

文章信息/Info

Title:
Fire-acupuncture Induces Proliferation and Differentiation of Melanocyte Stem Cells in Mouse Hair Follicle by Activating Wnt/β-catenin Signal
文章编号:
1001-7089(2020)06-0634-06
作者:
韩晓凤1徐伟1昌丽12陈茜1王禹毅1林茂1陶春蓉1龙娟1刁庆春1
1.重庆市中医院/重庆市第一人民医院皮肤科,重庆 400011; 2.重庆大学生物工程学院,重庆 400044
Author(s):
HAN Xiaofeng1XU Wei1CHANG Li12CHEN Xi1WANG Yuyi1LIN Mao1TAO Chunrong1LONG Juan1DIAO Qingchun1
(1.Department of Dermatology,Chongqing Traditional Chinese Medicine HospitalandChongqing First People's Hospital,Chongqing 400011,China; 2. College of Bioengineering,Chongqing University,Chongqing 400044,China)
关键词:
毫火针 毛囊黑素干细胞 Wnt/β-catenin信号通路
Keywords:
Fire-acupuncture Hair follicle melanocyte stem cells Wnt/β-catenin signal pathway
分类号:
R 758.71
DOI:
10.13735/j.cjdv.1001-7089.201909068
文献标志码:
A
摘要:
目的 探讨毫火针对小鼠毛囊黑素干细胞增殖分化的作用及机制。方法 将30只7周龄的C57BL/6J雌性小鼠随机分成5组,分别进行毫火针针刺0~4次,每3 d 1次,每次针刺结束24 h后取材。肉眼观察评估小鼠背部皮肤的变化情况,HE染色观察毛囊的形态变化,RT-PCR和Western blot分别检测TRP1、TRP2、MITF-M的mRNA水平和蛋白水平,RT-PCR检测LEF1、WNT4、WNT10a、β-catenin的mRNA水平。结果 随着火针次数的增加,小鼠背部颜色加深,毛囊从静止期逐渐进入生长期,TRP1、TRP2、MITF-M的蛋白水平和mRNA水平随着火针次数的增加而增加,LEF1、WNT4、WNT10a和β-catenin的mRNA水平随着火针次数增加呈上升趋势。结论 火针可通过激活Wnt/β-catenin信号促进小鼠毛囊黑素干细胞增殖分化。
Abstract:
Objective To investigate the effect and the mechanism of fire-acupuncture on melanocyte stem cells(MSCs)in mouse hair follicle.Methods Thirty 7-week-old female C57BL6/J mice were randomly divided into 5 groups which were subjected to fire-acupuncture for 0~4 times respectively.Each group was treated every 3 days.The mice were sacrificed 24 hours after the end of each fire-acupuncture.The changes of the dorsal skin was assessed visually.The morphological changes of the hair follicles were detected by HE staining.The mRNA and protein levels of TRP1,TRP2,MITF-M were measured by RT-RCR and Western blot respectively.The mRNA level of LEF1,WNT4,WNT10a and β-catenin were measured by RT-RCR.Results The color of the dorsal skin were deepened with an increase in the number of fire-acupuncture treatment.The hair follicles gradually entered anagen from telogen.With an increase in the number of fire-acupuncture treatment,the mRNA and protein levels of TRP1,TRP2 and MITF-M were significantly increased(P<0.05),and the mRNA level of LEF1,WNT4,WNT10a and β-catenin showed an increasing trend.Conclusion Fire-acupuncture promotes proliferation and differentiation of melanocyte stem cells of mouse hair follicle by activating Wnt/β-catenin signal.

参考文献/References:

[1] Qiu W,Chuong CM,Lei M.Regulation of melanocyte stem cells in the pigmentation of skin and its appendages:Biological patterning and therapeutic potentials[J].Exp Dermatol,2019,28(4):395-405.
[2] Lévesque M,Feng Y,Rebecca A,et al.Inflammation drives wound hyperpigmentation in zebrafish by recruiting pigment cells to sites of tissue damage[J].Dis Model Mech,2013,6(2):508-515.
[3] Chen CC,Wang L,Plikus MV,et al.Organ-level quorum sensing directs regeneration in hair stem cell populations[J].Cell,2015,161(2):277-290.
[4] 张颜,黄蜀,陈纯涛,等.火针加灸法联合窄谱中波紫外线治疗掌跖角化性湿疹的临床疗效观察[J].中国皮肤性病学杂志,2014,28(8):846-848.
[5] 白美娇,吴英楠,刘白雪,等.火针治疗白癜风的研究进展[J].国际中医中药杂志,2017,39(7):666-668.
[6] 韩晓凤,刘冬阳,陈茜,等.毫火针激活β-catenin信号促进小鼠毛发生长[J].中国皮肤性病学杂志,2019,33(4):405-408.
[7] Müller-Röver S,Handjiski B,van der Veen C,et al.A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages[J].J Invest Dermatol,2001,117(1):3-15.
[8] Hosaka C,Kunisada M,Koyanagi-Aoi M,et al.Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes[J].Pigment Cell Melanoma Res,2019,32(5):623-633.
[9] Vandamme N,Berx G.From neural crest cells to melanocytes:cellular plasticity during development and beyond[J].Cell Mol Life Sci,2019,76(10):1919-1934.
[10] Nishimura EK.Melanocyte stem cells:a melanocyte reservoir in hair follicles for hair and skin pigmentation[J].Pigment Cell Melanoma Res,2011,24(3):401-410.
[11] Yamada T,Hasegawa S,Inoue Y,et al.Wnt/β-catenin and kit signaling sequentially regulate melanocyte stem cell differentiation in UVB-induced epidermal pigmentation[J].J Invest Dermatol,2013,133(12):2753-2762.
[12] Tulic MK,Cavazza E,Cheli Y,et al.Innate lymphocyte-induced CXCR3B-mediated melanocyte apoptosis is a potential initiator of T-cell autoreactivity in vitiligo[J].Nat Commun,2019,10(1):2178.
[13] Lee BW,Schwartz RA,Hercogová J,et al.Vitiligo road map[J].Dermatol Ther,2012,25(Suppl 1):S44-56.
[14] Regazzetti C,Joly F,Marty C,et al.Transcriptional analysis of vitiligo skin reveals the alteration of WNT pathway:a promising target for repigmenting vitiligo patients[J].J Invest Dermatol,2015,135(12):3105-3114.
[15] Sun Q,Rabbani P,Takeo M,et al.Dissecting Wnt signaling for melanocyte regulation during wound healing[J].J Invest Dermatol,2018,138(7):1591-1600.
[16] Ocampo-Candiani J,Salinas-Santander M,Trevino V,et al.Evaluation of skin expression profiles of patients with vitiligo treated with narrow-band UVB therapy by targeted RNA-seq[J].An Bras Dermatol,2018,93(6):843-851.
[17] Yamada T,Hasegawa S,Inoue Y,et al.Wnt/β-catenin and kit signaling sequentially regulate melanocyte stem cell differentiation in UVB-induced epidermal pigmentation[J].J Invest Dermatol,2013,133(12):2753-2762.

备注/Memo

备注/Memo:
[基金项目] 重庆市自然科学基金(cstc2018jcyjAX0759,cstc2015jcyjA10113,cstc2019jcyj-msxmX0741,cstc2017jxj1130036); 国家自然科学基金(81503588)
[通信作者] 龙娟,E-mail: wenzhongdexin@163.com
[Corresponding author]LONG Juan,E-mail:wenzhongdexin@163.com
[网络首发时间]2020-4-22 9:02
[网络首发地址]http://kns.cnki.net/kcms/detail/61.1197.R.20200421.1450.001.html
更新日期/Last Update: 2020-06-05