[1]崔晶,金哲虎,金珊,等.多配体蛋白聚糖-1对瘢痕疙瘩成纤维细胞增殖及迁移的影响[J].中国皮肤性病学杂志,2020,(06):617-621.[doi:10.13735/j.cjdv.1001-7089.201908144]
 CUI Jing,JIN Zhehu,JIN Shan,et al.Effects of Syndecan-1 on Proliferation and Migration of Keloid Fibroblasts[J].The Chinese Journal of Dermatovenereology,2020,(06):617-621.[doi:10.13735/j.cjdv.1001-7089.201908144]
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多配体蛋白聚糖-1对瘢痕疙瘩成纤维细胞增殖及迁移的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年06期
页码:
617-621
栏目:
论著
出版日期:
2020-06-01

文章信息/Info

Title:
Effects of Syndecan-1 on Proliferation and Migration of Keloid Fibroblasts
文章编号:
1001-7089(2020)06-0617-05
作者:
崔晶金哲虎金珊南美兰崔婧博金承龙
延边大学附属医院皮肤科,吉林 延吉 133000
Author(s):
CUI JingJIN ZhehuJIN ShanNAN MeilanCUI JingboJIN Chenglong
(Department of Dermatology,Yanbian University Hospital,Yanji <133000,China)
关键词:
多配体蛋白聚糖-1 瘢痕疙瘩 成纤维细胞 细胞增殖 细胞迁移
Keywords:
SDC-1 Keloid Fibroblast Cell proliferation Cell migration
分类号:
R 751
DOI:
10.13735/j.cjdv.1001-7089.201908144
文献标志码:
A
摘要:
目的 探讨多配体蛋白聚糖-1(syndecan-1,SDC-1)在瘢痕疙瘩组织及成纤维细胞中表达情况及其对细胞增殖及迁移的影响。方法 采用免疫组织化学方法、qRT-PCR方法及Western blot法检测瘢痕疙瘩组织及成纤维细胞中的SDC-1蛋白表达情况。接下来通过转染SDC-1 siRNA序列,使SDC-1的表达下降。通过CCK-8法检测敲减SDC-1对瘢痕疙瘩成纤维细胞增殖的影响。Western blot法测定敲减SDC-1对迁移相关蛋白MMP-2和MMP-9蛋白表达的影响。结果 SDC-1在瘢痕疙瘩组织及成纤维细胞中呈高表达(P<0.05)。与NC siRNA组相比,SDC-1 siRNA组抑制瘢痕疙瘩成纤维细胞的增殖,同时抑制MMP-9蛋白的表达(P<0.05); 而两组间MMP-2蛋白表达差异无统计学意义(P>0.05)。结论 SDC-1在瘢痕疙瘩组织及成纤维细胞中高表达,敲减SDC-1可抑制瘢痕疙瘩成纤维细胞增殖及迁移相关蛋白的表达,这为探讨SDC-1与瘢痕疙瘩成纤维细胞增殖及迁移的相关研究提供了依据。
Abstract:
Objective To investigate the expression of syndecan-1(SDC-1)in keloid tissues and fibroblasts and its effects on cell proliferation and migration.Methods Expression of SDC-1 protein in keloid tissues and fibroblasts was detected by immunohistochemistry,qRT-PCR and Western blot.Then,expression of SDC-1 was decreased by transfection of siRNA SDC-1,and the effect of SDC-1 knockdown on cell proliferation of keloid fibroblasts was detected by CCK-8 assay.Western blot was used to detect the effect of SDC-1 knockdown on the expression of migration-related proteins MMP-2 and MMP-9.Results SDC-1 was significantly overexpressed in both keloid tissues and fibroblasts(P<0.05).Compared with the NC siRNA group,the proliferation of keloid fibroblasts was inhibited,and the expression of MMP-9 was suppressed in the SDC-1 siRNA group(P<0.05).However,the expression of MMP-2 protein did not differ significantly between NC siRNA group and SDC-1 siRNA group(P>0.05).Conclusion SDC-1 is significantly overexpressed in both keloid tissues and fibroblasts.SDC-1 knockdown inhibits the proliferation and migration of keloid fibroblasts,which may provide a basis for the study of relationship between SDC-1 and cell proliferation and migration in keloid fibroblasts.

参考文献/References:

[1] Kelsh RM,McKeown-Longo PJ,Clark RAF.EDA fibronectin in keloids create a vicious cycle of fibrotic tumor formation [J].J Invest Dermatol,2015,135(7):1714-1718.
[2] Shih B,Garside E,McGrouther DA,et al.Molecular dissection of abnormal wound healing processes resulting in keloid disease [J].Wound Repair Regen,2010,18(2):139-153.
[3] Ibrahim SA,Gadalla R,El-Ghonaimy EA,et al.Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3,Notch and EGFR signaling pathways [J].Mol Cancer,2017,16(1):57.
[4] Lunde IG,Herum KM,Carlson CC,et al.Syndecans in heart fibrosis [J].Cell Tissue Res,2016,365(3):539-552.
[5] Kliment CR,Englert JM,Gochuico BR,et al.Oxidative s-tress alters syndean-1 distribution in lungs with pulmonary fibrosis [J].J Biol Chem,2009,284(6):3537-3545.
[6] Regös E,Abdelfattah HH,Reszegi A,et al.Syndecan-1 inhibits early stages of liver fibrogenesis by interfering with TGFβ1 action and upregulating MMP14 [J].Matrix Biol,2018,68-69:474-489.
[7] Andrews JP,Marttala J,Macarak E,et al.Keloids:The paradigm of skin fibrosis-Pathomechanisms and treatment [J].Matrix Biol,2016,51:37-46.
[8] Jumper N,Paus R,Bayat A.Functional histopathology of keloid disease [J].Histol Histopathol,2015,30(9):1033-1057.
[9] Robles DT,Berg D.Abnormal wound healing:keloids [J].Clin Dermatol,2007,25(1):26-32.
[10] Stepp MA,Pal-Ghosh S,Tadvalkar G,et al.Syndecan-1 and its expanding list of contacts [J].Adv Wound Care(New Rochelle),2015,4(4):235-249.
[11] Szatmári T,Ötvös R,Hjerpe A,et al.Syndecan-1 in cancer:implications for cell signaling,differentiation,and prognostication [J].Dis Markers,2015,2015:796052.
[12] Bagabir RA,Syed F,Shenjere P,et al.Identification of a potential molecular diagnostic biomarker in keloid disease:Syndecan-1(CD138)is overexpressed in keloid scar tissue [J].J Invest Dermatol,2016,136(11):2319-2323.
[13] Gallo R,Kim C,Kokenyesi R,et al.Syndecans-1 and -4 are induced during wound repair of neonatal but not fetal skin [J].J Invest Dermatol,1996,107(5):676-683.
[14] Shi S,Zhong D,Xiao Y,et al.Syndecan-1 knockdown inhibits glioma cell proliferation and invasion by deregulating a c-src/FAK-associated signaling pathway [J].Oncotarget,2017,8(25):40922-40934.
[15] Ren Z,van Andel H,de Lau W,et al.Syndecan-1 promotes Wnt/β-catenin signaling in multiple myeloma by presenting Wnts and R-spondins [J].Blood,2018,131(9):982-994.
[16] Sayyad MR,Puchalapalli M,Vergara NG,et al.Syndecan-1 facilitates breast cancer metastasis to the brain [J].Breast Cancer Res Treat,2019,178(1):35-49.
[17] Chute C,Yang X,Meyer K,et al.Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases [J].Breast Cancer Res,2018,20(1):66.

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备注/Memo

备注/Memo:
[基金项目] 国家自然基金资助项目(81960561); 吉林省教育厅(吉教科合字[2016]第270号)
[通信作者] 金承龙,E-mail:15526770921@163.com
[Corresponding author]JIN Chenglong,E-mail:15526770921@163.com
[网络首发时间] 2020-4-15 11:46
[网络首发地址] http://kns.cnki.net/kcms/detail/61.1197.r.20200414.1148.003.html
更新日期/Last Update: 2020-06-05