[1]陆海涛,牛刚,赵运华,等.T-钙黏蛋白对小鼠皮下氮烯咪胺耐药黑素瘤B16F10细胞株的影响[J].中国皮肤性病学杂志,2020,(05):524-529.[doi:10.13735/j.cjdv.1001-7089.201909108]
 LU Haitao,NIU Gang,ZHAO Yunhua,et al.The Effects of T-cadherin on Dacarbazine-resistant Subcutaneous B16F10 Melanoma in Mice[J].The Chinese Journal of Dermatovenereology,2020,(05):524-529.[doi:10.13735/j.cjdv.1001-7089.201909108]
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T-钙黏蛋白对小鼠皮下氮烯咪胺耐药黑素瘤B16F10细胞株的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年05期
页码:
524-529
栏目:
论著
出版日期:
2020-05-01

文章信息/Info

Title:
The Effects of T-cadherin on Dacarbazine-resistant Subcutaneous B16F10 Melanoma in Mice
文章编号:
1001-7089(2020)05-0524-06
作者:
陆海涛1牛刚2赵运华3石凯行1郭健1刘利君1于淑萍1何磊1段昕所1
1.承德医学院附属医院,河北 承德 067000; 2隆化县医院,河北 隆化 068150; 3.承德市中心医院,河北 承德 067000
Author(s):
LU Haitao1NIU Gang2ZHAO Yunhua3SHI Kaihang1GUO Jian1LIU Lijun1YU Shuping1HE Lei1DUAN Xinsuo1
(1.Department of Dermatology,Affiliated Hospital of Chengde Medical College,Chengde 067000,China; 2.Longhua County Hospital,Longhua 068150,China; 3.Chengde Central Hospital,Chengde 067000,China)
关键词:
T-钙黏蛋白 黑素瘤 氮烯咪胺 耐药性
Keywords:
T-cadherin Malignant melanoma Dacarbazine Drug resistance
分类号:
R 739.5
DOI:
10.13735/j.cjdv.1001-7089.201909108
文献标志码:
A
摘要:
目的 观察T-钙黏蛋白(cadherin)对小鼠皮下氮烯咪胺(dacarbazine,DTIC)耐药黑素瘤B16F10细胞株的影响。方法 通过体外分步诱导法诱导B16F10氮烯咪胺耐药细胞株(DTIC-R B16F10)。采用CCK-8法检测DTIC-R B16F10细胞的增殖情况。将T-cadherin基因转染至DTIC-R B16F10细胞。免疫组织化学检测转染后T-cadherin的表达情况。培养DTIC-R B16F10细胞,分为6组:对照组、pEGFP-N1组、T-cadherin组、DTIC组、pEGFP-N1联合DTIC组和T-cadherin联合DTIC组。通过CCK-8法和Transwell小室实验检测T-cadherin与氮烯咪胺联合对DTIC-R B16F10细胞活性和迁移的影响。建立荷瘤小鼠模型,观察T-cadherin对小鼠皮下注射DTIC-R B16F10细胞后肿瘤生长的影响。采用析因分析法评价T-cadherin联合氮烯咪胺对细胞活性、迁移和小鼠皮下瘤生长的影响。结果 DTIC-R B16F10组、B16F10组与DTIC-R B16F10+DTIC组A值差异无统计学意义(P >0.05)。免疫组织化学检测表明细胞表达T-cadherin。T-cadherin联合DTIC组细胞活性、迁移细胞数及肿瘤重量均显著低于其他组(P<0.05)。析因分析显示,T-cadherin联合氮烯咪胺对DTIC-R B16F10的细胞活性、迁移和小鼠皮下瘤生长的抑制有协同作用。结论 T-cadherin可抑制黑素瘤小鼠皮下瘤的生长,增加黑素瘤对氮烯咪胺的敏感性。
Abstract:
Objective To observe the effects of T-cadherin on dacarbazine-resistant subcutaneous B16F10 melanoma in mice.Methods Dacarbazine(DTIC)resistance in B16F10(DTIC-R B16F10)was achieved by in vitro stepwise induction.Cell counting kit-8(CCK-8)was used to test the proliferation of DTIC-R B16F10 cells.T-cadherin gene was transfected into DTIC-R B16F10 cells.The expression of T-cadherin was measured by immunohistochemistry.DTIC-R B16F10 cells were cultured and divided into six groups:control group,pEGFP-N1 group,T-cadherin group,DTIC group,pEGFP-N1 combined with DTIC group and T-cadherin combined with DTIC group.The effects of T-cadherin combined with DTIC on cell viability and migration of DTIC-R B16F10 were determined by CCK8 and transwell assay,respectively.Tumor-bearing mice were established.The effect of T-cadherin on the kinetics of tumor growth after subcutaneous injection of DTIC-R B16F10 cells in mice was observed.Factorial analysis was used to evaluate the effects of T-cadherin combined with DTIC on cell viability,migration and growth of subcutaneous tumors in mice.Results There were no statistical differences in A value among DTIC-R B16F10 group,B16F10 group and DTIC-R B16F10+DTIC group.The expression of T-cadherin was measured by immunohistochemistry.The cell viability,cell migration and tumor weight were significantly lower in T-cadherin combined with DTIC group than in other groups(P<0.05).The factorial analysis showed that there were synergistic effects of T-cadherin and DTIC in inhibiting the cell viability,migration and growth of subcutaneous tumors in mice(P<0.05).Conclusion T-cadherin shows a significant inhibitory effect on the growth of subcutaneous melanoma in mice.T-cadherin can promote the sensitivity of melanoma to DTIC.

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备注/Memo

备注/Memo:
[通讯作者] 赵运华,E-mail:zhaoyhlt1984@sina.com
[Corresponding author] ZHAO Yunhua,E-mail:zhaoyhlt
1984@sina.com
更新日期/Last Update: 2020-05-11