[1]曹文婷,刘鑫,罗雯,等.UVB对系统性红斑狼疮患者外周血单个核细胞自噬相关转录组学的影响[J].中国皮肤性病学杂志,2020,(04):388-387.[doi:10.13735/j.cjdv.1001-7089.201908122]
　CAO Wenting,LIU Xin,LUO Wen,et al.Effects of UVB on Autophagy-related Transcriptomics in Peripheral Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2020,(04):388-387.[doi:10.13735/j.cjdv.1001-7089.201908122]
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UVB对系统性红斑狼疮患者外周血单个核细胞自噬相关转录组学的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年04期
页码:
388-387
栏目:
论著
出版日期:
2020-04-01

文章信息/Info

Title:
Effects of UVB on Autophagy-related Transcriptomics in Peripheral Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus
文章编号:
1001-7089(2020)04-0388-06
作者:
曹文婷1; 刘鑫2; 罗雯1; 邓丹琪1
1.昆明医科大学第二附属医院皮肤性病科,云南 昆明650101; 2.西部战区总医院(原成都军区总医院)皮肤科,四川 成都 100191
Author(s):
CAO Wenting1; LIU Xin2; LUO Wen1; DENG Danqi1
(1.Department of Dermatology and Venereology,the Second Affiliated Hospital of Kunming Medical University,Kunming 650101,China; 2.Department of Dermatology,the General Hospital of the Western Theater Command PLA,Chengdu 100191,China)
关键词:
红斑狼疮; 系统性;  中波紫外线;  自噬;  mRNA
Keywords:
Systemic lupus erythematosus;  Ultraviolet B;  Autophagy;  mRNA
分类号:
R 593.24+1
DOI:
10.13735/j.cjdv.1001-7089.201908122
文献标志码:
A
摘要:
目的 检测中波紫外线(UVB)照射对系统性红斑狼疮(SLE)患者的外周血单个核细胞(PBMC)自噬相关mRNA表达的影响并验证。方法 分离15例SLE患者和15例正常对照的PBMC,各分为UVB照射组和未照射组,照射UVB后培养24 h,提取细胞总RNA,使用RNA-Seq测序,筛选出各组间自噬相关差异性表达mRNA,并通过qPCR及Western blot对部分基因(ATG7、p62/SQSTM1)的表达情况进行验证。结果 RNA-Seq显示SLE患者未照射UVB组(SLE-C组)和正常人未照射UVB组(NC-C组)相比,共有266个基因上调,85个基因下调; SLE患者照射UVB组(SLE-UVB组)和正常人照射UVB组(NC-UVB组)相比,共有318个基因上调,146个基因下调; GO功能富集分析显示差异基因与自噬小体组装、巨自噬的正性调节、巨自噬的调节等有关。对ATG7和p62进行RT-qPCR和Western blot验证,结果显示ATG7和p62在SLE-UVB组的表达水平较SLE-C组和NC-UVB组高,与RNA-Seq的变化趋势一致。结论 获得SLE患者PBMC照射UVB前后的自噬相关mRNA表达数据,为进一步阐明UVB对SLE发病的影响提供了理论基础。
Abstract:
Objective To explorer the effect of ultraviolet B(UVB)irradiation on the expression of autophagy-related mRNAs in peripheral blood mononuclear cells(PBMC)of patients with systemic lupus erythematosus(SLE).Methods PBMCs from 15 patients with SLE and 15 normal controls were isolated and divided into UVB-irradiated and non-irradiated groups.After UVB irradiation for 24 hours,total RNA was extracted and sequenced by RNA-Seq.Differentially expressed of mRNAs were screened and part of which(ATG7,p62/SQSTM1)were further detected by qPCR and Western blot.Results RNA-Seq showed that 266 genes were up-regulated and 85 genes were down-regulated in PBMCs from SLE patients unexposed to UVB group(SLE-C group)compared with normal control non-UVB group(NC-C group),318 genes were up-regulated and 146 genes were down-regulated in PBMCs from SLE patients exposed to UVB group(SLE-UVB group)compared with normal control UVB group(NC-UVB group).GO function enrichment analysis showed that differential genes were involved in autophagosome assembly,positive regulation of macroautophagy,and regulation of macroautophagy.RT-qPCR and Western blot of ATG7 and p62 showed that the differentially expression levels of ATG7 and p62 in SLE-UVB group were higher than those in SLE-C group and NC-UVB group.Conclusion The differentially expressed autophagy-related mRNAs in PBMCs of patients with SLE may be a basis for further elucidation of the effect of UVB on the pathogenesis of SLE.

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备注/Memo

备注/Memo:
[基金项目] 国家自然科学基金项目(81460472,81660517,81860552); 云南省医疗卫生单位内设研究机构科研项目(2017NS312); 云南省应用基础研究昆医联合专项(2014FB050,2019FE001〔-148〕)
[作者单位] 1.昆明医科大学第二附属医院皮肤性病科,云南 昆明650101; 2.西部战区总医院(原成都军区总医院)皮肤科,四川 成都 100191
[通信作者] 邓丹琪,E-mail:danqid128@sina.com
[网络首发时间] 2020-03-13 14:58[网络首发地址] https://doi.org/10.13735/j.cjdv.1001-7089.201908122[Corresponding author] DENG Danqi,E-mail:danqid128@sina.com
更新日期/Last Update: 2020-04-30