[1]黄风云,胡凯,章媛华,等.KPNA2基因在皮肤黑素瘤中的表达及功能[J].中国皮肤性病学杂志,2020,(01):15-20.[doi:10.13735/j.cjdv.1001-7089.201903176]
 HUANG Fengyun,HU Kai,ZHANG Yuanhua,et al.Expression and Function of Gene KPNA2 in Cutaneous Malignant Melanoma[J].The Chinese Journal of Dermatovenereology,2020,(01):15-20.[doi:10.13735/j.cjdv.1001-7089.201903176]
点击复制

KPNA2基因在皮肤黑素瘤中的表达及功能
分享到:

《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2020年01期
页码:
15-20
栏目:
论著
出版日期:
2020-01-01

文章信息/Info

Title:
Expression and Function of Gene KPNA2 in Cutaneous Malignant Melanoma
文章编号:
1001-7089(2020)01-0015-06
作者:
黄风云1 胡凯1章媛华1孙孜孜1鲁文芳1孟祖东2
1.武汉市第四医院华中科技大学同济医学院附属武汉普爱医院皮肤科, 湖北 武汉 430030; 2.十堰市人民医院(湖北医药学院附属人民医院)皮肤科,湖北 十堰 442000
Author(s):
HUANG Fengyun1HU Kai 1ZHANG Yuanhua1SUN Zizi1LU Wenfang1MENG Zudong2
(1.Department of Dermatology,the Fourth Hospital of Wuhan City,Puai Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China; 2.Department of Dermatology,People's Hospital of Shiyan City,Hubei Medical College Affiliated People's Hospital,Shiyan 442000,China)
关键词:
KPNA2基因 黑素瘤 细胞增殖 预后
Keywords:
KPNA2 Melanoma Cell proliferation Prognosis
分类号:
R 739.5
DOI:
10.13735/j.cjdv.1001-7089.201903176
文献标志码:
A
摘要:
目的 研究KPNA2基因在皮肤黑素瘤中的生物学功能。方法 基因芯片分析KPNA2在皮肤黑素瘤中的表达,Real time PCR检测KPNA2基因在皮肤黑素瘤组织及细胞中的表达水平。小干扰RNA 转染A375细胞株,平板克隆、MTT法检测KPNA2基因敲低组、对照组皮肤黑素瘤细胞的增殖活性。Transwell实验计算皮肤黑素瘤细胞过膜数量,研究KPNA2与皮肤黑素瘤细胞迁移作用的关系。结果 KPNA2在皮肤黑素瘤组织中的表达显著高于癌旁皮肤组织,表达随着病理分级的恶性程度的升级而增高。高表达KPNA2组患者预后明显比KPNA2组患者差。平板克隆及MTT实验结果显示si-KPNA2组的细胞活性及增殖明显受抑制(P均<0.01),Transwell实验显示si-KPNA2组的细胞过膜数量(87.33±6.20)/视野,明显低于对照组(271.70±8.70)/视野,差异有统计学意义(P<0.01)。结论 KPNA2在皮肤黑素瘤组织表达升高,促进黑素瘤细胞的增殖和迁移,其可作为黑素瘤的潜在临床诊治靶点及预后标志物。
Abstract:
Objective To investigate the expression and biological functions of KPNA2 in cutaneous malignant melanoma.Methods Bioinformatic analysis of gene chip was used to identify the expression of KPNA2 in cutaneous malignant melanoma,further the expression level of KPNA2 in cutaneous malignant melanoma tissues was detected by real-time polymerase chain reaction(PCR).A375 cell line was transfected with small interfering RNA,and the proliferation viability of cutaneous malignant melanoma cells in KPNA2 overexpression group and control group were measured by cell colony formation assay and MTT assay.Transwell assay were performed to calculate the number of A375 cells passing through the membrane,and to analyze the relationship between KPNA2 and the migration of A375 cells.Results The expression of KPNA2 in cutaneous malignant melanoma tissues was significantly higher than that in adjacent normal tissues.The expression of KPNA2 in malignant melanoma tissues was increased with the escalation of the malignant degree of pathological stage.The prognosis of patients with high expression of KPNA2 was much more poor than patients with low expression of KPNA2.Cell colony formation assay and MTT assay showed that the cell viability and proliferation in si-KPNA2 group were significantly inhibited(P<0.01).Transwell assay showed that the number of A375 cells passing through the membrane in the si-KPNA2 group was lower than the control group(87.33±6.20/field vs.271.70±8.70/field).The difference was statistically significant(P<0.01).Conclusion Knockdown of KPNA2 expression can significantly inhibit the proliferation and migration of cutaneous malignant melanoma cells,suggesting that KPNA2 plays an important role in the proliferation and metastasis of melanoma,and it can be used as a potential clinical diagnosis and treatment target and prognostic marker for cutaneous malignant melanoma.

参考文献/References:

[1] Siegel RL,Miller KD,Jemal A.Cancer statistics,2018[J].CA Cancer J Clin,2018,68(1):7-30.
[2] Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[3] 姚战非,张雪梅,马枞.血管内皮生长因子及其受体在人皮肤黑素瘤的组学数据分析[J].中国皮肤性病学杂志,2019,33(6):628-633.
[4] Rastrelli M,Tropea S,Rossi CR,et al.Melanoma:epidemiology,risk factors,pathogenesis,diagnosis and classification[J].In Vivo,2014,28(6):1005-1011.
[5] 张德利,林茂,刁庆春.迷迭香酸对人表皮黑素细胞黑素生成的影响[J].中国皮肤性病学杂志,2019,33(6):634-637.
[6] 杜少静,贺慧颖.核转运蛋白KPNA2与肿瘤关系的研究进展[J].中国肿瘤临床,2016,43(17):780-784.
[7] Yu L,Wang G,Zhang Q,et al.Karyopherin alpha 2 expression is a novel diagnostic and prognostic factor for colorectal cancer[J].Oncol Lett,2017,13(3):1194-1200.
[8] Huang L,Zhou Y,Cao XP,et al.KPNA2 promotes migration and invasion in epithelial ovarian cancer cells by inducing epithelial-mesenchymal transition via Akt/GSK-3beta/Snail activation[J].J Cancer,2018,9(1):157-165.
[9] Alshareeda AT,Negm OH,Green AR,et al.KPNA2 is a nuclear export protein that contributes to aberrant localisation of key proteins and poor prognosis of breast cancer[J].Br J Cancer,2015,112(12):1929-1937.
[10] Ikenberg K,Valtcheva N,Brandt S,et al.KPNA2 is overexpressed in human and mouse endometrial cancers and promotes cellular proliferation[J].J Pathol,2014,234(2):239-252.
[11] Li J,Liu Q,Liu Z,et al.KPNA2 promotes metabolic reprogramming in glioblastomas by regulation of c-myc[J].J Exp Clin Cancer Res,2018,37(1):194.
[12] Gluz O,Wild P,Meiler R,et al.Nuclear karyopherin alpha2 expression predicts poor survival in patients with advanced breast cancer irrespective of treatment intensity[J].Int J Cancer,2008,123(6):1433-1438.
[13] Lin F,Gao L,Su Z,et al.Knockdown of KPNA2 inhibits autophagy in oral squamous cell carcinoma cell lines by blocking p53 nuclear translocation[J].Oncol Rep,2018,40(1):179-194.
[14] Tsai MM,Huang HW,Wang CS,et al.MicroRNA-26b inhibits tumor metastasis by targeting the KPNA2/c-jun pathway in human gastric cancer[J].Oncotarget,2016,7(26):39511-39526.
[15] Altan B,Yokobori T,Mochiki E,et al.Nuclear karyopherin-α2 expression in primary lesions and metastatic lymph nodes was associated with poor prognosis and progression in gastric cancer[J].Carcinogenesis,2013,34(10):2314-2321.

相似文献/References:

[1]高敏,余良,刘盛秀,等.皮肤激光共聚焦显微镜诊断和鉴别色素痣和黑素瘤评价[J].中国皮肤性病学杂志,2016,(07):699.[doi:10.13735/j.cjdv.1001-7089.201510023]
 GAO Min,YU Liang,LIU Sheng-xiu,et al.The Differential Diagnosis between Melanocytic Nevi and Melanomas using the Reflectance Confocal Microscopy[J].The Chinese Journal of Dermatovenereology,2016,(01):699.[doi:10.13735/j.cjdv.1001-7089.201510023]
[2]杨历辉,李东霞,苏依拉,等.小檗碱对人皮肤黑素瘤A375细胞周期相关miRNA的影响[J].中国皮肤性病学杂志,2016,(11):1123.[doi:10.13735/j.cjdv.1001-7089.201605119]
 YANG Li-hui,LI Dong-xia,SU Yi-la,et al.Inhibitory Effect of Berberine on Human Melanoma A375 Cells[J].The Chinese Journal of Dermatovenereology,2016,(01):1123.[doi:10.13735/j.cjdv.1001-7089.201605119]
[3]胡文婷,许爱娥.晕痣的研究进展[J].中国皮肤性病学杂志,2017,(07):800.[doi:10.13735/j.cjdv.1001-7089.201607001]
 HU Wen-ting,XU Ai-e.Research Progress in Halo Nevus[J].The Chinese Journal of Dermatovenereology,2017,(01):800.[doi:10.13735/j.cjdv.1001-7089.201607001]
[4]韩昭,张伶,刘钊,等.丹参酮ⅡA对黑素瘤A375细胞CXCR7及VEGF表达的影响[J].中国皮肤性病学杂志,2017,(11):1188.[doi:10.13735/j.cjdv.1001-7089.201706067]
 HAN Zhao,ZHANG Ling,LIU Zhao,et al.Effects of TanshinoneⅡA on CXCR7 and VEGF Expression in Malignant Melanoma A375 Cells[J].The Chinese Journal of Dermatovenereology,2017,(01):1188.[doi:10.13735/j.cjdv.1001-7089.201706067]
[5]贺勤,帅俊,陈加媛,等.转铁蛋白受体在皮肤黑素瘤中的检测[J].中国皮肤性病学杂志,2013,(04):337.
[6]汪峰,汪小柳,刘志军,等.慢病毒介导SOX9下调体外抑制人黑素瘤细胞MV3的转移潜能[J].中国皮肤性病学杂志,2019,(07):747.[doi:10.13735/j.cjdv.1001-7089.201812014]
 WANG Feng,WANG Xiaoliu,LIU Zhijun,et al.Lentivirus Mediated SOX9 Down-regulation Inhibits Metastatic Potential of Melanoma Cells MV3 in Vitro[J].The Chinese Journal of Dermatovenereology,2019,(01):747.[doi:10.13735/j.cjdv.1001-7089.201812014]
[7]龙剑文,罗晶,尹绪文.重楼皂苷Ⅰ通过抑制PI3K/Akt/mTOR通路诱导人黑素瘤A375细胞凋亡与自噬[J].中国皮肤性病学杂志,2019,(08):863.[doi:10.13735/j.cjdv.1001-7089.201812158]
 LONG Jianwen,LUO Jing,YIN Xuwen.Induces the Autophagy and Apoptosis of Melanoma A375 Cells by Suppressing PI3K/Akt/mTOR Signaling Pathway[J].The Chinese Journal of Dermatovenereology,2019,(01):863.[doi:10.13735/j.cjdv.1001-7089.201812158]
[8]李焰梅,郝丹,蒋献.大麻二酚在皮肤科的研究进展[J].中国皮肤性病学杂志,2019,(10):1202.[doi:10.13735/j.cjdv.1001-7089.201901008]
 LI Yanmei,HAO Dan,JIANG Xian.Research Progress of Cannabidiol in Dermatology[J].The Chinese Journal of Dermatovenereology,2019,(01):1202.[doi:10.13735/j.cjdv.1001-7089.201901008]
[9]陆海涛,牛刚,赵运华,等.T-钙黏蛋白对小鼠皮下氮烯咪胺耐药黑素瘤B16F10细胞株的影响[J].中国皮肤性病学杂志,2020,(05):524.[doi:10.13735/j.cjdv.1001-7089.201909108]
 LU Haitao,NIU Gang,ZHAO Yunhua,et al.The Effects of T-cadherin on Dacarbazine-resistant Subcutaneous B16F10 Melanoma in Mice[J].The Chinese Journal of Dermatovenereology,2020,(01):524.[doi:10.13735/j.cjdv.1001-7089.201909108]
[10]韩昭,王久江,张伶,等.microRNA-136基于Wnt信号通路在黑素瘤EMT中的作用[J].中国皮肤性病学杂志,2020,(02):128.[doi:10.13735/j.cjdv.1001-7089.201906014]
 HAN Zhao,WANG Jiujiang,ZHANG Ling,et al.Role of MicroRNA-136 in EMT of Melanoma Based on Wnt Signaling Pathway[J].The Chinese Journal of Dermatovenereology,2020,(01):128.[doi:10.13735/j.cjdv.1001-7089.201906014]

备注/Memo

备注/Memo:
[基金项目] 湖北省十堰市科学技术局引导性科研项目(18Y52); 湖北省卫生健康委员会指导性项目(WJ2019F048)
[作者单位] 1.武汉市第四医院华中科技大学同济医学院附属武汉普爱医院皮肤科, 湖北 武汉 430030; 2.十堰市人民医院(湖北医药学院附属人民医院)皮肤科,湖北 十堰 442000
[通信作者] 孟祖东,E-mail:doctor1968 @163.com
[网络首发时间] 2019-12-11 10:44[网络首发地址] https://doi.org/10.13735/j.cjdv.1001-7089.201903176[Corresponding author] MENG Zudong,E-mail:doctor1968 @163.com
更新日期/Last Update: 2020-01-30