[1]安月鹏,柏青松,袁锐,等.蜈蚣败毒饮对转基因银屑病模型皮损IL-17和IL-23蛋白表达的影响及与皮损指数的相关性[J].中国皮肤性病学杂志,2019,(05):575-579.[doi:10.13735/j.cjdv.1001-7089.201807195]
 AN Yuepeng,BAI Qingsong,YUAN Rui,et al.Influence of Wugongbaidu Decoction on Expression of IL-17 and IL-23 Protein in Psoriatic Lesion and its Correlation with MPASI in Transgenic Model of Psoriasis[J].The Chinese Journal of Dermatovenereology,2019,(05):575-579.[doi:10.13735/j.cjdv.1001-7089.201807195]
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蜈蚣败毒饮对转基因银屑病模型皮损IL-17和IL-23蛋白表达的影响及与皮损指数的相关性
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2019年05期
页码:
575-579
栏目:
中医中药
出版日期:
2019-04-01

文章信息/Info

Title:
Influence of Wugongbaidu Decoction on Expression of IL-17 and IL-23 Protein in Psoriatic Lesion and its Correlation with MPASI in Transgenic Model of Psoriasis
文章编号:
1001-7089(2019)05-0575-05
作者:
安月鹏1柏青松2袁锐1杨素清1
1.黑龙江中医药大学附属第一医院皮肤科,黑龙江 哈尔滨150040;2.黑龙江中医医药大学,黑龙江 哈尔滨150040
Author(s):
AN Yuepeng1 BAI Qingsong2 YUAN Rui1 YANG Suqing1
(1.Dermatology Department of First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin 150040, China;2.Heilongjiang University of Chinese Medicine, Harbin 150040, China)
关键词:
蜈蚣败毒饮银屑病IL-17IL-23蛋白
Keywords:
Wugongbaidu decoctionPsoriasisInterleukins-17Interleukins-23Protein
分类号:
R 758.63
DOI:
10.13735/j.cjdv.1001-7089.201807195
文献标志码:
B
摘要:
目的观察蜈蚣败毒饮对转基因银屑病模型小鼠皮损指数MPASI及皮损中IL-17和IL-23蛋白表达的影响,并对其相关性进行分析。方法将小鼠随机分为生理盐水空白对照组,甲氨蝶呤组,蜈蚣败毒饮低、中、高剂量组,分别行药物灌胃,采用改良的小鼠皮损面积及严重程度评分方法(MPASI)对银屑病小鼠的皮损进行系统评价,同时采用蛋白印迹法检测小鼠皮损中IL-17、IL-23的蛋白含量,并应用双变量线性回归分析对小鼠皮损指数和蛋白含量情况进行相关性评价。结果蜈蚣败毒饮高剂量组和甲氨蝶呤组的MPASI变化值最为明显(P<0.05),同时与其他各组相比较,上述两组具有显著的降低皮损中IL-17和IL-23蛋白含量的作用(P<0.05),且△MPASI与IL-17蛋白含量、△MPASI与IL-23蛋白含量均呈现出线性负相关性。结论蜈蚣败毒饮可以有效改善银屑病小鼠皮损,机制可能与降低Th17细胞通路相关蛋白在银屑病皮损中的表达有关。
Abstract:
Objective To observe the effects of Wugongbaidu decoction on mouse psoriasis area and severity index (MPASI) and expressions of IL-17 and IL-23 protein in psoriatic lesion in transgenic model of psoriasis, and to determine the correlation between the MPASI and expression levels of IL-17 and IL-23 protein.Methods Mice were randomly divided into blank controls treated with saline, methotrexate-treated controls, and groups treated with low, medium and high dose of Wugongbaidu decoction.All treatments were administered intragastrically.A modified MPASI method was used to evaluate the clinical scores.Western blotting was used to assess expression levels of IL-17 and IL-23 protein in psoriatic lesions.The correlation of skin lesion index and protein contents were determined using bivariate linear regression analysis.ResultsIn addition to marked reductions in expression levels of IL-17 and IL-23 protein, MPASI scores in high-dose Wugongbaidu decoction and Methotrexate treated groups also differed significantly from other treatments (P<0.05 for both MPASI and IL-17 & IL-23 content).Moreover, △MPASI correlated negatively with expression levels of both IL-17 & IL-23 protein.ConclusionWugongbaidu decoction can effectively improve psoriatic lesions in mouse model, likely by a mechanism of reducing expression levels of proteins associated with pathway in Th17 cells.

参考文献/References:


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备注/Memo

备注/Memo:
[基金项目]国家自然科学基金面上项目(81373639)
[作者单位]1.黑龙江中医药大学附属第一医院皮肤科,黑龙江 哈尔滨150040;2.黑龙江中医医药大学,黑龙江 哈尔滨150040
[通讯作者]杨素清,E-mail:ysq_6410@163.com
更新日期/Last Update: 2019-04-15