[1]刘洋,史丙俊,刁庆春,等.寻常型天疱疮外周血长链非编码RNA的差异性表达[J].中国皮肤性病学杂志,2019,(05):524-528.[doi:10.13735/j.cjdv.1001-7089.201806136]
 LIU Yang,SHI Bingjun,DIAO Qingchun,et al.Differential Expression of Long-chain Non-coding RNA in Peripheral Blood of Pemphigus Vulgaris[J].The Chinese Journal of Dermatovenereology,2019,(05):524-528.[doi:10.13735/j.cjdv.1001-7089.201806136]
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寻常型天疱疮外周血长链非编码RNA的差异性表达
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2019年05期
页码:
524-528
栏目:
论著
出版日期:
2019-04-01

文章信息/Info

Title:
Differential Expression of Long-chain Non-coding RNA in Peripheral Blood of Pemphigus Vulgaris
文章编号:
1001-7089(2019)05-0524-05
作者:
刘洋史丙俊刁庆春江雪钱思宇邓莉王小琴
重庆市第一人民医院皮肤科,重庆 400000
Author(s):
LIU Yang SHI BingjunDIAO Qingchun JIANG Xue QIAN Siyu DENG Li WANG Xiaoqin
(Department of Dermatology, Chongqing First People′s Hospital, Chongqing 400000,China)
关键词:
天疱疮 长链非编码RNA(LncRNA) 表达 差异性
Keywords:
PemphigusLong-chain non-coding RNA (LncRNA)ExpressionDifference
分类号:
R 758.66
DOI:
10.13735/j.cjdv.1001-7089.201806136
文献标志码:
A
摘要:
目的筛选寻常型天疱疮(PV)患者外周血单个核细胞(PBMC)和正常人PBMC中差异性表达的长链非编码RNA(LncRNA)。方法利用LncRNA表达谱芯片检测5例PV和3例正常人PBMC中LncRNA的表达,以P<0.05,且差异表达倍数大于2或小于0.5的条件筛选出差异性表达的LncRNA,并采用Real-time PCR进一步验证其在40例PV患者和40例正常人PBMC中的表达情况。结果与正常人相比,PV患者PBMC中差异性表达的LncRNA共有219个,其中上调142个,下调77个。选取的4个LncRNA在40例PV患者和40例正常人PBMC中的验证结果为:Lnc-BST1-1∶1,NR_023343,Lnc-ERICH1-9∶3表达上调(P<0.05),Lnc-CABIN1-1∶1表达下调(P<0.05)。与基因芯片检测结果相符。结论多个长链非编码RNA在PV患者外周血单个核细胞中异常表达,提示它们可能在PV的发生发展中起着重要的调控作用。
Abstract:
Objective To elucidate the differential expression of long-chain non-coding RNA (LncRNA) in peripheral blood mononuclear cells (PBMC) in patients with pemphigus vulgaris (PV) and normal human PBMC.MethodsLncRNA expression profiling microarray was used to detect the expression of LncRNA in 5 cases of PV and 3 cases of normal human PBMCs.Differential expression of LncRNA was screened for those with significance of P<0.05)and differences ≥2 or≤0.5 folds.Real-time PCR was used to further verify its expression in 40 cases of PV patients and 40 cases of normal PBMC.ResultsCompared with normal people, there were 219 LncRNAs differentially expressed in PBMCs of PV patients, including 142 up-regulated and 77 down-regulated.The validation of the four selected LncRNAs in 40 PV patients and 40 normal human PBMCs demonstrated upregulations of Lnc-BST1-1∶1, NR_023343 and Lnc-ERICH1-9∶3 expression (P<0.05), and down-regulation of lnc -CABIN1-1∶1 (P<0.05).This results were consistent with gene chip test results.ConclusionThere is a differential expression of LncRNA in PBMCs in PV patients compared with normal controls, indicating its important role in the development of PV.

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备注/Memo

备注/Memo:
[基金项目]重庆市科学技术委员会基础科学与前沿技术研究专项基金(cstc2016jcyjA0179)
[作者单位]重庆市第一人民医院皮肤科,重庆 400000
[通讯作者]王小琴,E-mail:Wangxq20032003@163.com
更新日期/Last Update: 2019-04-15