[1]周海永*,张传领,金百冶.淋病奈瑟菌的耐药性及分子流行病学观察[J].中国皮肤性病学杂志,2018,(12):1400-1403.[doi:10.13735/j.cjdv.1001-7089.201801174]
　ZHOU Haiyong*,ZHANG Chuanling,JIN Baiye.Drug Resistance and Molecular Epidemiology of Neisseria gonorrhoeae[J].The Chinese Journal of Dermatovenereology,2018,(12):1400-1403.[doi:10.13735/j.cjdv.1001-7089.201801174]
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淋病奈瑟菌的耐药性及分子流行病学观察
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2018年12期
页码:
1400-1403
栏目:
性传播疾病
出版日期:
2018-12-01

文章信息/Info

Title:
Drug Resistance and Molecular Epidemiology of Neisseria gonorrhoeae
作者:
周海永1; 2*; 张传领3; 金百冶1
1.浙江大学医学院附属第一医院泌尿外科,浙江 杭州 310003; 2.浙江医院泌尿外科,浙江 杭州 311202; 3.浙江萧山医院检验科,浙江 杭州 311202; *在职研究生
Author(s):
ZHOU Haiyong1; 2*; ZHANG Chuanling3; JIN Baiye1
(1.Department of Urinary Surgery,the First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China; 2.Department of Urinary Surgery,Zhejiang Hospital,Hangzhou 311202,China; 3.Department of Clinical Laboratory,Zhejiang Xiaoshan Hospital,Hangzhou 311202,China)
关键词:
淋病奈瑟菌;  感染;  耐药性;  NG-MAST
Keywords:
Neisseria gonorrhoeae;  Infection;  Drug resistance;  NG-MAST
分类号:
R 759.2
DOI:
10.13735/j.cjdv.1001-7089.201801174
文献标志码:
B
摘要:
目的 探讨临床分离的淋病奈瑟菌(淋球菌)对常用抗菌药物的耐药性及分子流行特征。方法 收集2015-2016年本院临床分离的淋球菌,采用琼脂稀释法检测青霉素、四环素、大观霉素、环丙沙星、头孢曲松、头孢克肟、阿奇霉素的最小抑菌浓度(MIC),头孢噻吩纸片法检测产青霉素酶淋球菌(PPNG),淋球菌多抗原序列(NG-MAST)确定菌株的ST。结果 共分离出126株淋球菌,对抗生素的耐药率依次为环丙沙星(100.00%)、青霉素(73.02%)、四环素(65.08%)、阿奇霉素(21.43%),检出17.46%(22/126)的阿奇霉素高水平耐药(AZ-HLR)(MIC≥256mg/L)菌株,未发现耐大观霉素、头孢曲松、头孢克肟的淋球菌。57.14%(72/126)的菌株为PPNG。NG-MAST分型结果显示,126株淋球菌分为82种不同的STs型别,其中ST1866(6.35%)、ST1927(5.56%)、ST3102(5.56%)是主要ST型,没有明显优势的ST型别。结论 本地区分离的淋球菌存在遗传背景的多样性; 阿奇霉素耐药性较高,且发现AZ-HLR菌株,不适合单独治疗淋球菌感染; 头孢曲松和大观霉素仍可作为淋病抗感染的一线药物。
Abstract:
Objective To investigate the antibiotic resistance and molecular epidemiology of clinically isolated Neisseria gonorrhoeae(NG).Methods A total of 126 NG strains were collected from 2015 to 2016 in Xiaoshan district,Hangzhou.Minimum inhibitory concentrations(MIC)to penicillin,tetracycline,spectinomycin,ciprofloxacin,ceftriaxone,cefixime and azithromycin were determined by the agar dilution method.Cefalotin paper discs were used for detection of penicillinase-producing NG(PPNG).ST of the strain was determined by Neisseria gonorrhoeae multi-antigen sequence typing(NG-MAST).Results Of the 126 NG strains,100.00% displayed resistance to ciprofloxacin,73.02% to penicillin,65.08% to tetracycline,and 21.43% to azithromycin.In addition,17.46% of isolates were azithromycin high-level resistance(AZ-HLR).No resistant strain was found for spectinomycin,ceftriaxone and cefixime.57.14%(72/126)of isolates were identified as PPNG.NG MAST analysis showed all of isolates were divided into 82 different STs types,of which ST1866(6.35%),ST1927(5.56%),and ST3102(5.56%)were the main ST types,and there was no obviously prevalent ST.Conclusion There was a diversity of genetic background in NGs from local population.The resistance rate of azithromycin was high and AZ-HLR isolates were found.Therefore,azithromycin is not preferable for the treatment of NG infection alone.Ceftriaxone and spectinomycin can still be used as the rst-line therapy against gonorrhoea.

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备注/Memo

备注/Memo:
[作者单位] 1.浙江大学医学院附属第一医院泌尿外科,浙江 杭州 310003; 2.浙江医院泌尿外科,浙江 杭州 311202; 3.浙江萧山医院检验科,浙江 杭州 311202; *在职研究生
[通讯作者] 金百冶,E-mail:1189006@zju.edu.cn
更新日期/Last Update: 2018-11-20