[1]高宏彬,李子卓,刘小明,等.特应性皮炎小鼠模型皮损中miR-223和TSLP的表达及分析[J].中国皮肤性病学杂志,2018,(06):638-645.[doi:10.13735/j.cjdv.1001-7089.201709150]
　GAO Hongbin,LI Zizhuo,LIU Xiaoming,et al.Expression and Analysis of miR-223 and TSLP in Skin Lesions of Mice Model with Atopic Dermatitis[J].The Chinese Journal of Dermatovenereology,2018,(06):638-645.[doi:10.13735/j.cjdv.1001-7089.201709150]
点击复制

特应性皮炎小鼠模型皮损中miR-223和TSLP的表达及分析
分享到：

《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2018年06期
页码:
638-645
栏目:
论著
出版日期:
2018-06-01

文章信息/Info

Title:
Expression and Analysis of miR-223 and TSLP in Skin Lesions of Mice Model with Atopic Dermatitis
作者:
高宏彬1; 2; 李子卓1; 2; 刘小明1; 黄海艳1; 陈小帆2; 窦侠1; 于波1
1.北京大学深圳医院,广东 深圳518036; 2.深圳北京大学香港科技大学医学中心,广东 深圳 518036
Author(s):
GAO Hongbin1; 2; LI Zizhuo1; 2; LIU Xiaoming1; HUANG Haiyan1; CHEN Xiaofan2; DOU Xia1; YU Bo1
1.Peking University Shenzhen Hospital,Shenzhen 518036,China; 2.Shenzhen PKU-HKUST Medical Center, Shenzhen 518036,China
关键词:
miR-223;  TSLP;  特应性皮炎
Keywords:
miR-223;  TSLP;  Atopic dermatitis(AD)
分类号:
R 758.2
DOI:
10.13735/j.cjdv.1001-7089.201709150
文献标志码:
A
摘要:
目的 通过测定miR-223和TSLP在MC903诱导特应性皮炎小鼠模型皮损中的表达,分析两者在特应性皮炎发病中的相关性,阐明miR-223在特应性皮炎发病过程中的作用及其与TSLP的关系。方法 建立MC903诱导的特应性皮炎小鼠模型,检测模型皮损中miR-223和TSLP的相对表达量,进一步在miR-223基因敲除鼠中分析TSLP的表达变化及疾病严重程度的改变。结果 在WT鼠中,经MC903诱导的模型组较对照组皮损中miR-223和TSLP表达均明显上调,但在miR-223-/-鼠模型组皮损中miR-223和TSLP表达均下调,且特应性皮炎炎症反应减轻。结论 miR-223加重特应性皮炎的炎症反应,miR-223可能通过调节TSLP的表达参与小鼠特应性皮炎的发病过程。
Abstract:
Objective To detect the expressions of miR-223 and TSLP in skin lesions of MC903-induced mice model with atopic dermatitis, to analyze their correlation in the pathogenesis of atopic dermatitis and to elucidate the role of miR-223 in the pathogenesis of atopic dermatitis and its relationship with TSLP. Methods The mice model of atopic dermatitis induced by MC903 was established, and the relative expression levels of miR-223 and TSLP in the model skin lesions were detected. Furthermore, the changes in TSLP expression level and the severity of AD were analyzed in miR-223 knockout mice. Results Compared with the control group of C57BL/6J mice, the expressions of miR-223 and TSLP were significantly up-regulated in the skin lesions of the model group in C57BL/6J mice. However, the expressions of miR-223 and TSLP were down-regulated in the skin lesions of the model group in miR-223-/- mice, and the inflammatory response of atopic dermatitis was alleviated in the model group of miR-223-/- mice. Conclusion miR-223 aggravates the inflammatory response in atopic dermatitis, so that miR-223 may be involved in the pathogenesis of atopic dermatitis in mice by regulating TSLP expression.

参考文献/References:

[1] Xu F,Yan S,Li F,et al.Prevalence of childhood atopic dermatitis: an urban and rural community-based study in Shanghai,China[J].PLoS One,2012,7(5):e36174.
[2] Spergel JM.Epidemiology of atopic dermatitis and atopic march in children[J].Immunol Allergy Clin North Am,2010,30(3):269-280.
[3] 赵巍,刘俊峰,陈达灿,等.半抗原诱导特应性皮炎小鼠模型的研究进展[J].中国皮肤性病学杂志,2015,29(8):855-857.
[4] Mack GS.MicroRNA gets down to business[J].Nat Biotechnol, 2007, 25(6):631-638.
[5] Lu TX,Rothenberg ME.Diagnostic, functional, and therapeutic roles of microRNA in allergic diseases[J].J Allergy Clin Immunol, 2013, 132(1):3-13.
[6] 王奕,王克玉.复杂皮肤病的microRNAs相关性研究进展[J].中国皮肤性病学杂志,2016,28(11):1168-1171.
[7] Sonkoly E, Janson P, Majuri ML, et al.MiR-155 is overexpressed in patients with atopic dermatitis and modulates T-cell proliferative responses by targeting cytotoxic T lymphocyte-associated antigen 4[J]. J Allergy Clin Immunol,2010,126(3):581-589.
[8] Vennegaard MT,Bonefeld CM,Hagedorn PH,et al.Allergic contact dermatitis induces upregulation of identical microRNAs in humans and mice [J].Contact Dermatitis,2012,67(5):298-305.
[9] 王璨,王晓钰,于曦等.卡泊三醇诱导小鼠特应性皮炎模型的最适条件探索[J].中国药理学通报,2016,32(7):1027-1032.
[10] Zhang Z,Hener P,Frossard N,et al.Thymic stromal lymphopoietin overproduced by keratinocytes in mouse skin aggravates experimental asthma[J].Proc Natl Acad Sci U S A,2009,106(5):1536-1541.
[11] Soumelis V,Reche PA,Kanzler H,et al.Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP[J].Nat Immunol,2002,3(7):673-680.
[12] 瞿旻晔,袁晓琳,马健.特应性皮炎发病过程及其机制研究现状探讨[J].中国皮肤性病学杂志,2015,29(10):1085-1087.
[13] Tatsuno K, Fujiyama T, Yamaguchi H,et al.TSLP directly interacts with skin-homing Th2 cells highly expressing its receptor to enhance IL-4 production in atopic dermatitis[J].J Invest Dermatol, 2015, 135(12):3017-3024.
[14] Liu YJ.Thymic stromal lymphopoietin: master switch for allergic inflammation[J].J Exp Med,2006,203(2):269-273.
[15] 宫泽琨,杨雨潮,解奎霞,等.特应性皮炎患儿外周血胸腺基质淋巴细胞生成素与白介素-25的表达及相关性[J].中国皮肤性病学杂志,2016, 30(1):17-19.
[16] Liu TY, Chen SU, Kuo SH,et al.E2A-positive gastric MALT lymphoma has weaker plasmacytoid infiltrates and stronger expression of the memory B-cell-associated miR-223: possible correlation with stage and treatment response[J].Mod Pathol,2010,23(11):1507-1517.
[17] Laios A, O'Toole S, Flavin R,et al. Potential role of miR-9 and miR-223 in recurrent ovarian cancer[J].Mol Cancer,2008,7:35-72.
[18] Wong QW, Lung RW, Law PT, et al.MicroRNA-223 is commonly repressed in hepatocellular carcinoma and potentiates expression of Stathmin1[J].Gastroenterology,2008,135(1):257-269.
[19] Eyholzer M, Schmid S, Schardt JA,et al.Complexity of miR-223 regulation by CEBPA in human AML[J].Leuk Res,2010,34(5):672-676.
[20] Johnnidis JB, Harris MH, Wheeler RT,et al.Regulation of progenitor cell proliferation and granulocyte function by microRNA-223[J].Nature,2008,451(7182):1125-1129.
[21] Fazi F,Racanicchi S,Zardo G,et al.Epigenetic silencing of the myelopoiesis regulator microRNA-223 by the AML1/ETO oncoprotein[J].Cancer Cell,2007,12(5):457-466.
[22] Yuan JY, Wang F, Yu J,et al.MicroRNA-223 reversibly regulates erythroid and megakaryocytic differentiation of K562 cells[J].J Cell Mol Med,2009,13(11-12):4551-4559.
[23] Wang H,Chao K,Ng SC,et al. Pro-inflammatory miR-223 mediates the cross-talk between the IL23 pathway and the intestinal barrier in inflammatory bowel disease[J]. Genome Biol,2016,17:58-72.
[24] Fulci V, Scappucci G, Sebastiani GD, et al.miR-223 is overexpressed in T-lymphocytes of patients affected by rheumatoid arthritis[J].Hum Immunol,2010,71(2):206-211.
[25] Wang JF, Yu ML, Yu G,et al.Serum miR-146a and miR-223 as potential new biomarkers for sepsis[J].Biochem Biophys Res Commun,2010,394(1):184-188.
[26] Lu Han,Buchan Rachel,Cook Stuart.MicroRNA-223 regulates Glut4 expression and cardiomyocyte glucose metabolism[J].Cardiovasc Res,2010,86(3):410-420.
[27] Yu CH,Xu CF,Li YM.Association of MicroRNA-223 expression with hepatic ischemia/reperfusion injury in mice[J].Dig Dis Sci,2009,54(11): 2362-2366.

备注/Memo

备注/Memo:
[基金项目] 国家自然科学基金(81472878,81502727); 广东省自然科学基金(2014A030313723)
[通讯作者] 于波,E-mail:yubomd@163.com
更新日期/Last Update: 2018-06-01