[1]韩昭,张伶,刘钊,等.丹参酮ⅡA对黑素瘤A375细胞CXCR7及VEGF表达的影响[J].中国皮肤性病学杂志,2017,(11):1188-1191.[doi:10.13735/j.cjdv.1001-7089.201706067]
 HAN Zhao,ZHANG Ling,LIU Zhao,et al.Effects of TanshinoneⅡA on CXCR7 and VEGF Expression in Malignant Melanoma A375 Cells[J].The Chinese Journal of Dermatovenereology,2017,(11):1188-1191.[doi:10.13735/j.cjdv.1001-7089.201706067]
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丹参酮ⅡA对黑素瘤A375细胞CXCR7及VEGF表达的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2017年11期
页码:
1188-1191
栏目:
论著
出版日期:
2017-11-01

文章信息/Info

Title:
Effects of TanshinoneⅡA on CXCR7 and VEGF Expression in Malignant Melanoma A375 Cells
作者:
韩昭张伶刘钊徐艳艳张建忠牛亮李小静
河北工程大学附属医院皮肤科,河北 邯郸 056000
Author(s):
HAN Zhao ZHANG Ling LIU Zhao XU Yan-yan ZHANG Jian-zhong NIU Liang LI Xiao-jing
Department of Dermatology, Affiliated Hospital of Hebei University of Engineering, Handan 056002, China
关键词:
丹参酮ⅡA 黑素瘤 CXCR7 VEGF
Keywords:
Tanshinone ⅡA Malignant melanoma CXCR7 VEGF
分类号:
R 739.5
DOI:
10.13735/j.cjdv.1001-7089.201706067
文献标志码:
A
摘要:
目的 观察丹参酮ⅡA对黑素瘤A375细胞CXCR7及VEGF表达的影响,探讨其抗肿瘤的作用机制。方法 体外培养黑素瘤A375细胞,经4组不同浓度丹参酮ⅡA(0,1,2,4mg/L)作用后,ELISA法检测培养液中VEGF浓度,Real time-PCR法及Western blotting法分别检测其对CXCR7和VEGF mRNA及蛋白表达的影响。结果 丹参酮ⅡA处理黑素瘤A375细胞48h后,培养液中VEGF浓度随丹参酮ⅡA浓度的升高而降低,CXCR7及VEGF mRNA及蛋白表达随丹参酮ⅡA浓度的升高而降低,差异具有统计学意义(P<0.05)。结论 丹参酮ⅡA能抑制黑素瘤肿瘤细胞内VEGF及CXCR7的转录和蛋白的表达,提示丹参酮ⅡA可能具有潜在的抗肿瘤作用。
Abstract:
Objective To observe the influence of Tanshinone ⅡA on CXCR7 and VEGF expression in malignant melanoma A375 cells,and investigate its anti-tumor mechanism.Methods Cultured malignant melanoma A375 cells in vitro were divided into 4 groups to be treated with tanshinone ⅡA at different concentrations(0,1,2,4mg/L).VEGF concentration in the nutrient solution was detected by ELISA.The effects of CXCR7 and VEGF mRNA/protein expression were detected by Real time-PCR and Western Blotting respectively.Results A375 cells were treated with Tanshinone ⅡA for 48h.The concentration of VEGF in culture medium decreased with the increase of tanshinone ⅡA concentration,The expression of CXCR7 and VEGF mRNA/protein decreased with the increase of tanshinoneⅡA concentration,The difference was statistically significant(P<0.05).Conclusion Tanshinone ⅡA can inhibit the transcription and protein expression of VEGF and CXCR7 in melanoma tumor cells, suggesting that tanshinone ⅡA may have potential anti-tumor effects.

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备注/Memo

备注/Memo:
[通讯作者] 李小静,E-mail:zlmdsh@126.com
更新日期/Last Update: 2017-11-01