[1]李蒙,石磊,王秀丽,等.普鲁士蓝纳米粒子光热治疗皮肤黑素瘤的实验观察[J].中国皮肤性病学杂志,2017,(09):941-945.[doi:10.13735/j.cjdv.1001-7089.201701055]
 LI Meng,SHI Lei,WANG Xiu-li,et al.The Study of Prussian Blue Nanoparticles Photothermal Therapy on Skin Melanoma[J].The Chinese Journal of Dermatovenereology,2017,(09):941-945.[doi:10.13735/j.cjdv.1001-7089.201701055]
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普鲁士蓝纳米粒子光热治疗皮肤黑素瘤的实验观察
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2017年09期
页码:
941-945
栏目:
论著
出版日期:
2017-09-01

文章信息/Info

Title:
The Study of Prussian Blue Nanoparticles Photothermal Therapy on Skin Melanoma
作者:
李蒙12石磊2王秀丽2魏志平1
1.徐州医科大学附属医院皮肤科,江苏 徐州 221002; 2.上海市皮肤病医院、同济大学医学院光医学研究所, 上海 200443
Author(s):
LI Meng12 SHI Lei2 WANG Xiu-li2 WEI Zhi-ping1
1.Department of Dermatology,Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002, China; 2.Institute of Photomedicine,Shanghai Skin Disease Hospital,Tongji University School of Medicine, Shanghai 200443, China
关键词:
肿瘤 皮肤黑素瘤 普鲁士蓝纳米粒子 光热治疗
Keywords:
Tumor Skin melanoma Prussian blue nanoparticles Photothermal therapy
分类号:
R 739.5
DOI:
10.13735/j.cjdv.1001-7089.201701055
文献标志码:
A
摘要:
目的 探讨普鲁士蓝纳米粒子(PB NPs)光热治疗皮肤黑素瘤的疗效与安全性。方法 CCK-8法测定普鲁士蓝纳米粒子光热治疗前后对皮肤黑素瘤细胞B16F10的增殖抑制作用。用C57BL/6小鼠建立皮肤黑素瘤模型,并将其随机分为对照组、PB NPs组、激光组和联合治疗组。治疗过程中,使用热成像仪观察对照组和联合治疗组肿瘤区域的温度变化。各组小鼠均连续观察10d。每日测量瘤体大小,比较四组瘤体体积变化。治疗后第10天,对照组和联合治疗组分别取小鼠的心肝脾肺肾进行HE染色,比较PB NPs对小鼠内脏毒副作用的影响。结果 CCK-8法测得B16F10细胞分别与不同浓度PB NPs(250μg/mL,125μg/mL,62μg/mL,31μg/mL,16μg/mL,8μg/mL,0μg/mL)培养24h的细胞增殖,每组细胞存活率都在70%以上。808nm NIR激光强度1W/cm2照射5min治疗B16F10细胞后,250μg/mL PB NPs细胞死亡率为71.80%。治疗10d后,联合治疗组小鼠瘤体体积明显小于其余三组小鼠瘤体体积; 各组小鼠瘤体体积两两进行比较,联合治疗组小鼠瘤体消失,且差异均有统计学意义(P<0.05); HE染色观察到普鲁士蓝纳米粒子对小鼠脏器无明显毒副作用。结论 普鲁士蓝纳米粒子联合光热治疗可以有效安全治疗皮肤黑素瘤。
Abstract:
Objective To investigate the effects and safety of prussian blue nanoparticles(PB NPs)photothermal therapy(PTT)on skin melanoma.Methods The inhibitory rate of PB NPs before and after the treatment of PTT on B16F10 cells was measured by CCK-8 method.Tumor bearing C57BL/6 mice were randomly divided into four groups: control group, PB NPs group, laser only group, combined treatment group.The temperature changes of the tumors were observed in the control group and the combined treatment group by using a thermal imager.The mice in each group had been observed for ten days,and each day the size of the tumor had also been recorded. Then the comparison of tumor volime were made in four groups,respectively.Hematoxylin and eosin(HE)staining of heart, liver, spleen, lung and kidney were carried out after laser irradiations for 10 days.Results B16F10 cells were cultured with PB NPs(250μg/mL, 125μg/mL, 62μg/mL, 31μg/mL, 16μg/mL, 8μg/mL and 0μg/mL)for 24h.The cell survival rate of each group was above 70%.After incubation with 250μg/mL PB NPs and exposure to 808nm laser light(1W/cm2)for 5min, the mortality of B16F10 cells was 71.80%.Ten days after treatment, combined treatment group mice tumors had disappeared, the differences between each group were statistically significant.HE staining of heart, liver, spleen, lung and kidney indicated that PB NPs possess excellent in vivo biocompatibility.Conclusion PB NPs with PTT can effectively treat skin melanoma.

参考文献/References:

[1] Zhang K,Wong P,Salvaggio C, et al.Synchronized targeting of Notch and ERBB signaling suppresses melanoma tumor growth through inhibition of Notch1 and ERBB3[J].J Invest Dermatol,2016,136(2):464-472.
[2] Chartrain M,Riond J,Stennevin A,et al.Melanoma chemotherapy leads to the selection of ABCB5-expressing cells[J].PLo S One, 2012, 7(5):e36762.
[3] Tang T, Eldabaje R, Yang L.Current Status of biological therapies for the treatment of metastatic melanoma [J].Anticancer Res,2016,36(7):3229-3241.
[4] Kim H,Chung K, Lee S,et al.Near-infrared light-responsive nanomaterials for cancer theranostics[J].Wiley Interdiscip Rev Nanomed Nanobiotchol, 2016,8(1):23-45.
[5] Liu Y, Xu M, Chen Q, et al.Gold nanorods/mesoporous silica-based nanocomposite as theranostic agents for targeting near-infrared imaging and photothermal therapy induced with laser[J].Int J Nanomedicine,2015,28(10):4747-4761.
[6] Tian Q,Tang M, Sun Y, et al.Hydrophilic flower-like CuS superstructures as an efficient 980 nm laser-driven photothermal agent for ablation of cancer cells[J].Adv Mater, 2011, 23(31):3542-3547.
[7] Huang X,Tang S, Mu X, et al.Freestanding palladium nanosheets with plasmonic and catalytic properties[J].Nat Nanotechnol, 2011, 6(1):28-32.
[8] Liu Z, Zhao F, Gao S, et al.The applications of gold nanoparticle-initialed chemiluminescence in biomedical detection[J].Nanoscale Res Lett,2016,11(1):460.
[9] Navarro JR, Manchon D, Lerouge F, et al.Synthesis of PEGylated gold nanostars and bipyramids for intracellular uptake[J].Nanotechnology, 2012,23(46): 465602.
[10] Kim JW, Galanzha EI, Shashkov EV, et al.Golden carbon nanotubes as multimodal photoacoustic and photothermalhighcontrast molecular agents[J].Nat Nanotechnol,2009,4(10):688-694.
[11] Cheng L, Liu J, Gu X, et al.PEGylated WS2 nanosheets as a multifunctional theranostic agent for in vivo dual-Modal CT/Photo-acoustic imaging guided photothermal therapy[J].Adv Mater, 2014,26(12):1886-1893.
[12] Li W, Zamani R, Rivera GP, et al.CuTe nanocrystals: shape and size control, plasmonic properties, and use as SERS probes and photothermal agents[J].J Am Chem Soc,2013,135(19):7098-7101.
[13] Cheng L, Gong H, Zhu W, et al.PEGylated prussian blue nanocubes as a theranosticagent for simultaneous cancer imaging and photothermal therapy[J].Biomaterials,2014,35(37):9844-9852.
[14] Kobayashi H, Watanabe R, Choyke PL.Improving conventional enhanced permeability and retention(EPR)effects; what is the appropriate target?[J].Theranostics, 2013, 4(1): 81-89.
[15] Jo SD, Ku SH, Won YY, et al.Targetednanotheranostics for future personalized medicine: recent progress in cancer therapy[J].Theranostics, 2016,6(9): 1362-1377.

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备注/Memo

备注/Memo:
[基金项目] 上海申康医院发展中心临床科技创新项目-市级医院新兴前沿技术联合攻关项目(SHDC12015123); 国家自然科学基金项目(81472538)
[通讯作者] 魏志平,E-mail:xzwzp1961@aliyun.com
更新日期/Last Update: 2017-09-10