[1]朱邦勇,李伟,陈绍椿,等.广西地区淋病奈瑟菌对头孢菌素的耐药性及多抗原测序分型[J].中国皮肤性病学杂志,2017,(08):865.[doi:10.13735/j.cjdv.1001-7089.201702065]
 ZHU Bang-yong,LI Wei,CHEN Shao-chun.Cephalosporin Resistance of Neisseria Gonorrhoeae and its Multi-antigen Sequence Typing in Guangxi Zhuang Autonomous Region[J].The Chinese Journal of Dermatovenereology,2017,(08):865.[doi:10.13735/j.cjdv.1001-7089.201702065]
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广西地区淋病奈瑟菌对头孢菌素的耐药性及多抗原测序分型
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2017年08期
页码:
865
栏目:
性传播疾病
出版日期:
2017-08-01

文章信息/Info

Title:
Cephalosporin Resistance of Neisseria Gonorrhoeae and its Multi-antigen Sequence Typing in Guangxi Zhuang Autonomous Region
作者:
朱邦勇1李伟1陈绍椿2等等
1.广西皮肤病防治研究所,广西 南宁 530007; 2.中国疾病预防控制中心性病控制中心,江苏 南京 210042; 3.广西医科大学第一附属医院,广西 南宁 530021; 4.玉林市皮肤病防治医院,广西 玉林 537006; 5.贵港市皮肤病防治院,广西 贵港 537100; 6.北海市人民医院,广西 北海 536100; 7.武鸣更昌医院,广西 南宁 530100
Author(s):
ZHU Bang-yong1 LI Wei1 CHEN Shao-chun2……
1.Guangxi Institute of Dermatology, Nanning 530003, China; 2.National Center for STD Control,China CDC,Nanjing 210042, China; 3.The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China; 4.Yulin Institute of Dermatology, Yulin 537
关键词:
淋病奈瑟菌 耐药 最小抑菌浓度 淋病奈瑟菌多抗原序列分型
Keywords:
Neisseria gonorrhoeae Drug resistance Minimum inhibitory concentration(MIC) Neisseria gonorrhoeae multi-antigen sequence typing(NG-MAST)
分类号:
R 759.2
DOI:
10.13735/j.cjdv.1001-7089.201702065
文献标志码:
B
摘要:
目的 了解广西地区淋病奈瑟菌对两种头孢菌素药物的耐药性及对应菌株多抗原测序分型(NG-MAST)情况。方法 从2012-2014年广西地区性病门诊患者尿道或宫颈收集分泌物培养共195株淋病奈瑟菌,经过分离纯化及鉴定后,用琼脂稀释法测定其对头孢曲松和头孢克肟的最小抑菌浓度(MIC),并进行淋病奈瑟菌多抗原测序分型(NG-MAST),数据统计采用SPSS13.0软件分析。结果 2012-2014年从性病门诊淋病患者中收集临床分离菌株195株,其对头孢曲松的MIC≥0.060mg/L的菌株比例为8.21%; 对头孢克肟的MIC≥0.060mg/L的菌株比例分别为11.28%。NG-MAST分型结果显示,195株淋病奈瑟菌共有147个型别,型别多样性很高,其中118种为已知型别,29种为新型别。所有菌株中以ST10380(n=5),ST621(n=4),ST1927(n=4),ST2318(n=4),ST3741(n=4),ST4539(n=4),ST1768(n=3),ST2770(n=3),ST6312(n=3)为优势型别。结论 广西地区淋病奈瑟菌NG-MAST型别具有丰富多样性,某些型别与头孢曲松和头孢克肟的MIC值有较强对应关系。NG-MAST分型可用于本地区淋病奈瑟菌耐药监测的辅助工具。
Abstract:
Objective To investigate resistance of Neisseria gonorrhoeae(NG)isolates to ceftriaxone and cefixime in Guangxi Zhuang Autonomous region, and their NG multi-antigen sequence typing(NG-MAST)genotypes.Methods A total of 195 NG strains were collected from the urethra or cervix at Sexually Transmitted Diseases clinics from 2012 to 2014 in Guangxi Zhuang Autonomous region. The minimum inhibitory concentrations(MIC)of ceftriaxone and cefixime were determined using agar dilution method. DNA was extracted from these strains with the Qiagen commercial kit and used for NG-MAST. Statistical analysis was performed with SPSS13.0.Results MIC of ≥0.060mg/L for ceftriaxone and cefixine were 8.2% and 11.3%, respectively. NG-MAST identified 147 genotypes, including 118 reported and 29 unreported genotypes. The prevalences of genotypes arranged in descending order were ST10380(n=5), ST621(n=4),ST1927(n=4),ST2318(n=4),ST3741(n=4),ST4539(n=4),ST1768(n=3),ST2770(n=3)and ST6312(n=3).Conclusion NG exhibits multiple genotypes in Guangxi Zhuang Autonomous region. Certain genotypes are strongly associated with MIC of ceftriaxone or cefixime. NG-MAST can facilitate monitoring the drug resistance of Neisseria gonorrhoeae.

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备注/Memo

备注/Memo:
[基金项目] 广西医药卫生适宜技术研究与开发项目(S201401-01) [通讯作者] 李伟,E-mail:lw17611981@163.com
更新日期/Last Update: 2017-08-01