[1]凡慧,王继辉,范萍,等.滋阴清热药对狼疮鼠肾组织自噬基因atg16L1和细胞焦亡炎症因子的影响[J].中国皮肤性病学杂志,2017,(06):602-606.[doi:10.13735/j.cjdv.1001-7089.201611152]
 FAN Hui,WANG Ji-hui,FAN Ping,et al.Effects on Autophagy Gene atg16L1 and Pyroptosis Immune Inflammatory Factors in Nephridial Tissue of Lupus Mice with Nourishing Yin Clearing Heat Herbs[J].The Chinese Journal of Dermatovenereology,2017,(06):602-606.[doi:10.13735/j.cjdv.1001-7089.201611152]
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滋阴清热药对狼疮鼠肾组织自噬基因atg16L1和细胞焦亡炎症因子的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2017年06期
页码:
602-606
栏目:
论著
出版日期:
2017-06-01

文章信息/Info

Title:
Effects on Autophagy Gene atg16L1 and Pyroptosis Immune Inflammatory Factors in Nephridial Tissue of Lupus Mice with Nourishing Yin Clearing Heat Herbs
作者:
凡慧1王继辉2范萍1梁康礼1胡小倩1陈金中3程喜平1
1.广州医科大学附属第一医院,广东 广州510120; 2.佛山市第一人民医院,广东 佛山528000; 3.复旦大学生命科学院遗传所遗传工程国家重点实验室,上海200433
Author(s):
FAN Hui1WANG Ji-hui2FAN Ping1LIANG Kang-li1HU Xiao-qian1CHEN Jin- zhong3CHENG Xi-ping1
1.The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China; 2. The First Hospital of Foshan, Foshan 528000, China; 3.State Key Laboratory of Genetic Engineering,Institute of Genetics,School of Life Sciences, Fudan University, Shanghai 200433, China
关键词:
滋阴清热药 自噬 细胞焦亡 足细胞靶抗原 红斑狼疮
Keywords:
Nourishing Yin Clearing Heat Herbs Autophagy Pyroptosis Podocyte target antigen Lupus erythematosus
分类号:
R 593.24
DOI:
10.13735/j.cjdv.1001-7089.201611152
文献标志码:
A
摘要:
目的 探讨滋阴清热药对狼疮鼠肾组织自噬基因atg16L1和细胞焦亡炎症因子的影响。方法 以滋阴清热方干预狼疮鼠,免疫组织化学方法观察自噬基因atg16L1肾组织中的组织形态,定量PCR检测atg16L1基因表达,同时检测肾组织细胞焦亡上下游炎症因子Caspase-1,IL-1β以及足细胞靶抗原Nephrin,Actinin4。结果 滋阴清热药能提高狼疮模型鼠组肾组织自噬基因atg16L1的表达(P<0.01),降低细胞焦亡炎症因子IL-1β(P<0.01)以及足细胞靶抗原Nephrin表达(P<0.05)。结论 滋阴清热药能调控狼疮鼠肾组织自噬基因atg16L1,减轻免疫炎症和器官损害。
Abstract:
Objective To observe the effect on autophagy gene atg16L1 and pyroptosis immune inflammatory factors in nephridial tissue of lupus mice with Nourishing Yin Clearing Heat Herbs. Methods Application with Nourishing Yin Clearing Heat Herbs observed the histomorphology of atg16L1,pyroptosis immune inflammatory factors Caspase-1,IL-1β and podocyte target antigen Nephrin,Actinin4 positive cells in nephridial tissue by immunohistochemical method, detected atg16L1, Caspase-1,IL-1β,Nephrin and Actinin4 gene expression by quantitative PCR.Results Nourishing Yin Clearing Heat Herbs could increase the autophagy gene atg16L1 expression of lupus mice nephridial tissue(P<0.01), decrease pyroptosis immune inflammatory factors IL-1β(P<0.01)and podocyte target antigen Nephrin(P<0.05)expression of lupus mice in nephridial tissue.Conclusion Nourishing Yin and Clearing Heat Herbs can regulate autophagy gene atg16L1 and reduce immune inflammation and organ damage.

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备注/Memo

备注/Memo:
[基金项目] 国家自然基金资助面上项目(项目批准号:81373649,81673983); 广东省自然基金资助项目(项目批准号:2014A030313504)
[通讯作者] 程喜平,E-mail:cxpgz@126.com
更新日期/Last Update: 2017-06-05