[1]王奇飒,孙东杰,何黎,等.重楼总皂苷及不同皂苷成分对痤疮相关病原菌抑菌效果的评价[J].中国皮肤性病学杂志,2016,(09):899-901.[doi:10.13735/j.cjdv.1001-7089.201602102]
　WANG Qi-sa,SUN Dong-jie,HE Li,et al.Evaluation of the Inhibitory Effect of Total Saponins of Polyphylla and its Fraction on Acne-associated Pathogens[J].The Chinese Journal of Dermatovenereology,2016,(09):899-901.[doi:10.13735/j.cjdv.1001-7089.201602102]
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重楼总皂苷及不同皂苷成分对痤疮相关病原菌抑菌效果的评价
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2016年09期
页码:
899-901
栏目:
论著
出版日期:
2016-09-01

文章信息/Info

Title:
Evaluation of the Inhibitory Effect of Total Saponins of Polyphylla and its Fraction on Acne-associated Pathogens
作者:
王奇飒1; 孙东杰1; 何黎1; 徐丹1; 吴文娟1; 李谦1; 刘海洋2; 杜艳3
1.昆明医科大学第一附属医院皮肤科,云南 昆明 650031; 2.中国科学院昆明植物所,云南 昆明 650031; 3.昆明医科大学第一附属医院检验科,云南 昆明 650031
Author(s):
WANG Qi-sa1; SUN Dong-jie1; HE Li1; XU Dan1; WU Wen-juan1; LI Qian1; LIU Hai-yang2; DU Yan3
1.Department of Dermatology,First Affiliated Hospital of Kunming Medical University,Kunming 650031,China; 2.Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650031,China; 3.Department of Clinical Laboratory,First Affiliated Hospital of Kunming Medical University,Kunming 650031,China
关键词:
重楼皂苷;  痤疮;  痤疮丙酸杆菌;  表皮葡萄球菌;  金黄色葡萄球菌;  最小抑菌浓度
Keywords:
Polyphylla;  Acne;  Propionibacterium acnes;  Staphylococcus epidermidis;  Staphylococcus aureus;  Minimum inhibitory concentration
分类号:
R 758.73+3
DOI:
10.13735/j.cjdv.1001-7089.201602102
文献标志码:
A
摘要:
目的 研究重楼总皂苷及不同重楼皂苷成分Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅶ,H对痤疮相关病原菌(痤疮丙酸杆菌、表皮葡萄球菌和金黄色葡萄球菌)的抑菌效果。方法 通过液体微量稀释法检测重楼总皂苷及不同皂苷成分对痤疮相关病原菌的最小抑菌浓度(MIC)。结果 重楼总皂苷对痤疮丙酸杆菌NCTC737,ATCC6919,表皮葡萄球菌ATCC12228,金葡菌ATCC6538的MIC分别为2.5,5.0,5.0,1.25mg/mL; 重楼皂苷Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅶ,H对痤疮丙酸杆菌NCTC737的MIC分别为0.6,1.2,2.5,5.0,2.5,2.5,10.0mg/mL; 对表皮葡萄球菌ATCC12228的MIC分别为1.2,1.2,5.0,5.0,2.5,2.5,10.0mg/mL; 对金葡菌ATCC6538的MIC分别为0.6,0.6,2.5,5.0,2.5,2.5,10.0mg/mL。结论 重楼总皂苷具有较好抑制痤疮相关病原菌的作用; 重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ在体外对痤疮发病相关菌具有明确的抑制作用,其中重楼皂苷Ⅰ的抑菌作用最强。
Abstract:
Objective To study the inhibitory effect of total saponins, polyphylla Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅶ,and H on acne-associated pathogens(Propionibacterium acnes,Staphylococcus epidermidis and Staphylococcus aureus).Methods Liquid microdilution method was used to determine the minimal inhibitory concentration(MIC)of total saponins and its fractions on P.acnes,S.epidermidis and S.aureus's. Results MICs of total saponins on Propionibacterium acnes NCTC737,ATCC6919,Staphylococcus epidermidis ATCC12228,Staphylococcus aureus ATCC6538 were 2.5,5.0,5.0,1.2mg/mL, respectively; MICs of polyphylla Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅶ and H on Propionibacterium acnes NCTC737 were 0.6,1.2,2.5,5,2.5,2.5,10.0mg/mL respectively; MICs of polyphylla Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅶ and H on Staphylococcus epidermidis ATCC12228 were 1.2,1.2,5,5,2.5,2.5,10.0mg/mL respectively,while their MICs on Staphylococcus aureus ATCC6538 were 0.6,0.6,2.5,5,2.5,2.5,10.0mg/mL respectively.Conclusion Total saponins of polyphylla can effectively inhibit acne-associated pathogens; polyphylla Ⅰ,Ⅱ,Ⅵ and Ⅶ, in particularly polyphylla I, also effectively inhibit acne-associated pathogens in vitro.

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备注/Memo

备注/Memo:
[基金项目] 省应用基础研究(昆医联合专项,2014FB045)[通讯作者] 孙东杰,E-mail:sundongjieder@aliyun.com
更新日期/Last Update: 2016-09-20