[1]伍慧媚,吴玮,卢传坚,等.应用ClinProt技术筛查寻常性银屑病不同中医证型患者的相关蛋白表达特征[J].中国皮肤性病学杂志,2015,(03):304-306,310.[doi:10.13735/j.cjdv.1001-7089.201408030]
 WU Hui mei,WU Wei,LU Chuan jian,et al.Applying ClinProt Technology to Detect the Expression Characteristic of Proteins in Psoriatic Vulgaris with Different Syndrome Patterns[J].The Chinese Journal of Dermatovenereology,2015,(03):304-306,310.[doi:10.13735/j.cjdv.1001-7089.201408030]
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应用ClinProt技术筛查寻常性银屑病不同中医证型患者的相关蛋白表达特征
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2015年03期
页码:
304-306,310
栏目:
中医中药
出版日期:
2015-03-01

文章信息/Info

Title:
Applying ClinProt Technology to Detect the Expression Characteristic of Proteins in Psoriatic Vulgaris with Different Syndrome Patterns
作者:
伍慧媚吴玮卢传坚胡坤华晏卉李挺滨姚丹霓
广东省中医院皮肤科,广东 广州510120
Author(s):
WU HuimeiWU WeiLU ChuanjianHU KunhuaYAN HuiLI TingbinYAO Danni
Guangdong Province Hospital of Traditional Chinese Medicine, Guangzhou 510120,China
关键词:
银屑病中医证型蛋白组学ClinProt技术
Keywords:
Psoriasis TCM syndrome Proteomics ClinProt Technique
分类号:
R 758.63
DOI:
10.13735/j.cjdv.1001-7089.201408030
文献标志码:
B
摘要:
目的应用ClinProt技术联合MALDITOF技术,检测正常人和不同中医证型寻常性银屑病患者血浆及血T淋巴细胞膜上的相关蛋白。方法选择血热证、血瘀证、血燥证的寻常性银屑病患者各10例,同时选取10例健康人作为对照组,采用ClinProt技术联合MALDITOF技术建立血浆和T淋巴细胞膜差异蛋白图谱进行对比。结果与对照组的T淋巴细胞膜蛋白相比,血热组有1个表达上调的差异蛋白峰(4 747D),血瘀组有1个表达上调(4 747D)、2个表达下调的差异蛋白峰(2 068D、7 764D),血燥组有1个表达上调的差异蛋白峰(4 747D)。而对照组和不同证型银屑病患者的血浆差异蛋白相比较,血热组有3个表达上调的差异蛋白峰(6 631D,9 288D,4 965D),血瘀组有5个表达上调的差异蛋白峰(2 863D,4 418D,6 631D,4 965D,3 241D),血燥组有2个表达上调的差异蛋白峰(4 965D,9 288D)和1个表达下调的差异蛋白峰(3 957D)。结论不同证型寻常性银屑病患者与健康人相比,血T淋巴细胞膜上及血浆中存在差异蛋白峰,这些蛋白可能与各个证型寻常性银屑病的发病机制有关。
Abstract:
ObjectiveTo detect plasma proteins and T Lymphocytes membrane proteins in normal people and psoriasis vulgaris patients with different syndrome patterns by using ClinProt combine with MALDITOF technologies. MethodsTen cases of bloodheat pattern, bloodstagnation pattern and blooddryness psoriasis vulgaris patients were enrolled, respectively. Meanwhile, 10 cases of healthy people were also recruited as control group. ClinProt combined with MALDITOF technologies were used to establish the different proteomic map of plasma protein and T lymphocytes membrane protein. ResultsComparing with healthy group, one differential T lymphocytes membrane protein upregulated peak(4 747D) was noted in bloodheat group, along with one upregulated peak (4 747D) and two downregulated peaks (2 068D, 7 764D) in bloodstagnation group, and one upregulated peak(4 747D) in blooddryness group. Comparing control group with psoriasis patients with different patterns, 3 upregulated differential plasma protein peak (6 631D,9 288D,4 965D) were noted in bloodheat group, along with 5 upregulated peak (2 863D,4 418D,6 631D,4 965D,3 241D) in bloodstagnation group, 2 upregulated peak (4 965D,9 288D) and 1 downregulated peak (3 957D) in blooddryness group. ConclusionPsoriasis vulgaris patients with different syndrome patterns have different T lymphocytes membrane and plasma differential protein peaks compared with healthy people, and these proteins might have a relationship with the pathogenesis of psoriasis vulgaris with different syndrome patterns.

参考文献/References:

[1] ElKomy M, Amin I, Zidan A, et al.Insulinlike growth factor1 in psoriatic plaques treated with PUVA and methotrexate[J].Journal of the European Academy of Dermatology and Venereology,2011,25(11):1288-1294.
[2] Rubant SA, Ludwig RJ, Diehl S, et al.Dimethylfumarate reduces leukocyte rolling in vivo through modulation of adhesion molecule expression[J]. Journal of Investigative Dermatology, 2008,128(2):326-331.
[3] 赵辨.中国临床皮肤病学[M].江苏科学技术出版,2009:1008-1025.
[4] 中医皮肤科常见病诊疗指南.中华中医药学会[M].中国中医药出版社,2012:1-2.
[5] Butt C, Lim S, Greenwood C, et al.VEGF,FGF1,FGF2 and EGF gene polymorphisms and psoriatic arthritis[J].BMC Musculoskelet Disord,2007,4(8):1.
[6] Heegaard NH, Larsen MR, Muncrief T,et al.Heterogeneous nuclear ribonucleoproteins C1/C2 identified as autoantigens by biochemical and mass spectrometric methods[J].Arthritis Research,2000,2(5):407-414.
[7] Van Swelm RP, Laarakkers CM, KooijmansOtero M, et al.Biomarkers for methotrexateinduced liver injury: urinary protein profiling of psoriasis patients[J]. Toxicology Letters,2013,221(3):219-224.
[8] 刘占奎,谭升顺,于春水,等.血清蛋白组学技术筛选寻常性银屑病相关蛋白的初步研究[J].中国皮肤性病学杂志,2007,11(21):644-650.

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备注/Memo

备注/Memo:
[项目基金]广东省科技计划项目(2011B031700028)
更新日期/Last Update: 2015-03-25