[1]张丽霞,曾波,王倩,等.胸腺基质淋巴细胞生成素在特应性皮炎小鼠模型中的作用[J].中国皮肤性病学杂志,2013,(10):26-29.
 ZHANG Li-xia,ZENG Bo,WANG Qian,et al.Experimental Study on the Effects of Thymic Stromal Lymphopoietin in Mouse Atopic Dermatitis Model[J].The Chinese Journal of Dermatovenereology,2013,(10):26-29.
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胸腺基质淋巴细胞生成素在特应性皮炎小鼠模型中的作用
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2013年10期
页码:
26-29
栏目:
论著
出版日期:
2013-10-01

文章信息/Info

Title:
Experimental Study on the Effects of Thymic Stromal Lymphopoietin in Mouse Atopic Dermatitis Model
作者:
张丽霞1曾波2王倩1卢葳1何迅1陈金1
1.四川省医学科学院四川省人民医院皮肤病性病研究所,四川 成都 610072; 2.四川省江油市人民医院皮肤科,四川 江油 621700
Author(s):
ZHANG Li-xia1 ZENG Bo2WANG Qian1LU Wei1HE Xun1CHEN Jin1
(1.The Institute of Dermatology and Venereology, Sichuan Provincial People's Hospital & Sichuan Academy of Medical Science, Chengdu 610072, China; 2.Department of Dermatology, Jiangyou People's Hospital, Jiangyou 621700, China)
关键词:
特应性皮炎 胸腺基质淋巴细胞生成素 小鼠模型
Keywords:
Atopic dermatitis Thymic stromal lymphopoietin Mice model
分类号:
R 758.2
文献标志码:
A
摘要:
目的 初步探讨胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin, TSLP)在特应性皮炎发病机制中的作用。方法 将32只BALB/c小鼠随机分为两组:对照组、实验组,每组16只,以2,4-二硝基氟苯丙酮/橄榄油溶液经皮肤致敏建立小鼠特应性皮炎模型。采用免疫组织化学方法检测小鼠皮损中TSLP的表达。ELISA法测定血清中TSLP,IL-4,IFN-γ及IgE的水平。结果 特应性皮炎(atopic dermatitis, AD)小鼠皮损中TSLP阳性表达物质主要位于胞核,胞浆及胞膜无特异性着色,实验组平均光密度值(74.306±17.898)明显高于对照组(46.093±3.347),差异有统计学意义(P<0.05)。AD小鼠血清TSLP,IL-4,IFN-γ及IgE(213.621±34.666,97.831±15.882,70.999±9.604,142.488±16.352)的水平明显高于对照组(151.997±22.328,60.560±9.286,51.224±4.534,14.851±3.971),差异均有统计学意义(P均<0.05)。结论 TSLP在特应性皮炎小鼠血清及皮损中大量表达,在AD的发病中可能发挥重要作用。
Abstract:
Objective To explore the role of thymic stromal lymphopoietin(TSLP)in the pathogenesis of atopic dermatitis.Methods Thirty-two BALB/c mice were randomly divided into control group and experimental group. The atopic dermatitis model was established by topical applications of 2, 4-Dinitrofluorobenzene in acetone/olive oil. The expression of TSLP in the lesions was evaluated by immunohistochemical staining. The serum levels of TSLP, IL-4, IFN-γ and IgE were determined by enzyme-linked immunosorbent assay(ELISA). ResultsPositive staining was mainly localized in the nucleus of the epidermal cells without specificstaining in cytoplasm and membrane. The intensity of TSLP staining in experimental group was significantly higher than that in the controls(74.306±17.898 vs. 46.093±3.347). The serum levels of TSLP, IL-4, IFN-γ and IgE in experimental group were also higher than that in the controls(213.621±34.666 vs. 151.997±22.328 for TSLP; 97.831±15.882 vs. 60.560±9.286 for IL-4; 70.999±9.604 vs. 51.224±4.534 for IFN-γ; 142.488±16.352 vs. 14.851±3.971 for IgE).Conclusion The expression levels of TSLP were higher in both AD lesions and serum. TSLP may play a key role in the pathogenesis of atopic dermatitis.

参考文献/References:

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备注/Memo

备注/Memo:
[基金项目] 四川省卫生厅科研课题基金项目(100469) [作者单位] 1.四川省医学科学院四川省人民医院皮肤病性病研究所,四川 成都 610072; 2.四川省江油市人民医院皮肤科,四川 江油 621700
更新日期/Last Update: 2013-10-20