[1]张媛,张荣平,张志毕,等.龙血竭凝胶对兔耳痤疮模型TNF-α、IL-6和MFGE8的影响[J].中国皮肤性病学杂志,2021,(01):30-e814dd43-ab41-4110-95db-b71f526e3137/pdf.[doi:10.13735/j.cjdv.1001-7089.202004104]
　ZHANG Yuan,ZHANG Rongping,ZHANG Zhibi,et al.Effect of Resina Draconis Gel on TNF-α,IL-6 Level in Serum and MFGE8 Expression in the Skin from Rabbit's Ear Acne Model[J].The Chinese Journal of Dermatovenereology,2021,(01):30-e814dd43-ab41-4110-95db-b71f526e3137/pdf.[doi:10.13735/j.cjdv.1001-7089.202004104]
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龙血竭凝胶对兔耳痤疮模型TNF-α、IL-6和MFGE8的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年01期
页码:
30-e814dd43-ab41-4110-95db-b71f526e3137/pdf
栏目:
论著
出版日期:
2021-01-01

文章信息/Info

Title:
Effect of Resina Draconis Gel on TNF-α,IL-6 Level in Serum and MFGE8 Expression in the Skin from Rabbit's Ear Acne Model
文章编号:
1001-7089(2021)01-0030-07
作者:
张媛1; 张荣平2; 张志毕1; 杨晖1; 杨丽晗1; 王元3; 唐莹4
1.昆明医科大学生物医学工程研究中心,云南昆明650500;2.云南中医药大学药学院,云南昆明650500;3.昆明学院,云南昆明6502144;4.昆明医科大学科技成果孵化中心电镜室,云南昆明650500
Author(s):
ZHANG Yuan1; ZHANG Rongping2; ZHANG Zhibi1; YANG Hui1; YANG Lihan 1; WANG Yuan3; TANG Ying4
1.Biomedical Engineering Research Center of Kunming Medical University,Kunming 650500,China; 2.College of Pharmacy in Yunnan University of Chinese Medicine,Kunming 650500,China; 3.Kunming University,Kunming 650214,China; 4.Science and Technology Incubation Center of Electron Microscopy Laboratory in Kunming Medical University,Kunming 650500,China
关键词:
龙血竭凝胶;  痤疮;  肿瘤坏死因子-α;  白介素-6;  乳脂肪球表皮生长因子Ⅷ
Keywords:
RDC gel;  Acne;  TNF-α;  IL-6;  MFGE8
分类号:
R 758.73+3
DOI:
10.13735/j.cjdv.1001-7089.202004104
文献标志码:
A
摘要:
目的 探讨龙血竭凝胶对兔耳痤疮模型TNF-α、IL-6和MFGE8的作用效果。方法 24只新西兰家兔随机分为空白组4只和模型组20只,模型组以煤焦油涂抹和痤疮丙酸杆菌接种制备兔耳痤疮模型后,随机分为模型组、阳性药组、龙血竭凝胶高、中、低剂量组[根据对痤疮丙酸杆菌(P.acne)、金黄色葡萄球菌(S.aureus)、表皮葡萄球菌(S.epidermidis)的MIC确定],每组4只,涂抹给药,空白组和模型组给予空白凝胶基质,持续14 d。观察兔耳厚度及病理组织结构,ELISA法检测血清中TNF-α、IL-6含量,Western blot检测耳片组织中MFGE8表达。结果 龙血竭凝胶对痤疮丙酸杆菌的MIC为62.5 mg/mL,对金黄色葡萄球菌的MIC为125 mg/mL,对表皮葡萄球菌的MIC为31.25 mg/mL。故以31.25、62.5、125 mg/mL为低、中、高剂量给药,14 d后发现,中、高剂量龙血竭凝胶可不同程度改善大鼠耳廓痤疮模表皮角化、减轻炎性细胞浸润及脓性丘疹。阳性对照组和龙血竭凝胶高剂量组兔耳厚度与模型相比均有降低(P<0.05),兔血清中TNF-α、IL-6水平与模型组相比均有降低(P<0.01),空白组、阳性对照组和高剂量组耳片组织中MFGE8表达高于模型组(P<0.01)。结论 外用涂擦龙血竭125 mg/mL、62.5 mg/mL凝胶对兔耳痤疮有改善作用,这可能与其能够降低其血清中TNF-α、IL-6水平,恢复痤疮模型兔耳组织中MFGE8的表达有关。
Abstract:
Objective Study the effect of the resin from Dracaena cochinchinensis(RDC)gel on the TNF-α,IL-6 and the Milk Fat Globule-EGF-factor 8(MFGE8)in acne model of rabbits' serum and ear skin. Methods Twenty-four New Zealand rabbits were randomly divided into blank group with 4 rabbits and model group with 20 rabbits. Model group was built rabbit ear acne model with coal tar daub and acne propionic acid bacillus. After the success of the acne model,the rabbits divided into 5 groups including model group,positive control group(Adapalene gel)and the low,medium and high dose RDC gel groups,with 4 rabbits for each group. The blank group was given a blank gel matrix. Daubing lasted 14 days for once a day. Observed the appearance and the pathological organization structure of rabbit ears. The thickness was measured with Vernier caliper. The level of TNF-α,IL-6 in serum was detected by ELISA,and MFGE8 expression in the rabbits' ear skin tissue was analyzed by Western blot. Results 31.25,62.5,125 mg/mL RDC gel was tested in the experimental groups,which was based on the MIC result to S.epidermidis,P.acne,S.aureus and After 14 days,the medium and high dose RDC gel relieved auricle acne epidermal keratinization,reduced inflammatory cell infiltration and purulent papules. The ear thickness of the positive control group and RDC gel high dose group were lower than the model of group(P<0.05),and the rabbit serum TNF-a,IL-6 levels are also lower than the model group(P<0.01). Compared with the model group,the MFGE8 expression in ears for the blank group,positive control group and high dose group was higher(P<0.01),and there were no significant differences in the three groups. Conclusion 125 mg/mL,62.5 mg/mL RDC gel were effective in the treatment of acne. It may be not only related to the inhibition and reduction of TNF-α and IL-6 production,but also enhanced MFGE8 expression in acne model rabbits' ears.

参考文献/References:

[1] 张荣平,王兴华,赵荣华,等.南药研究系列丛书:龙血竭[M].北京:人民卫生出版社,2017:172-183.
[2] 黄捷,赖伟珍,王太,等.龙血竭胶囊治疗寻常型痤疮的临床研究[J].中国热带医学,2006,6(7):1200-1201.
[3] 欧柏生,刘卫兵.龙血竭胶囊治疗寻常型痤疮70例[J].中国民间疗法,2005,13(9):36-37.
[4] 于小兵,吴晓金,桑旭东,等.对痤疮患者生活质量影响因素的分析[J].中国麻风皮肤病杂志,2006,22(10):816-818.
[5] Newton JN,Mallon E,Klassen A,et al.The effectiveness of acne treatment:an assessment by patients of the outcome of therapy[J].Br J Dermatol,1997,137(4):563-567.
[6] 刘文彬,梁庆,王晖,等.一种痤疮复合动物模型的建立[J].广东药学院学报,2014,30(2):198-201.
[7] 刘婧,王思农,肖静秋,等.加味黑布膏对大鼠耳廓复合痤疮模型局部组织IL-2及EGF的影响[J].中国皮肤性病学杂志,2020,34(1):89-93.
[8] 王海琦,崔鸿峥,李畅,等.月见草油调节p38MAPK、NF-κB信号通路抗炎治疗痤疮的实验[J].中药药理与临床,2018,34(2):62-67.
[9] 郑玉梅,孟若琳,彭海悦,等.青少年痤疮面部皮肤微生物群落结构变化[J].微生物学通报,2019,46(12):3414-3423.
[10] 犹忠萍,曹碧兰.痤疮丙酸杆菌抗生素耐药性研究进展[J].中国皮肤性病学杂志,2018,32(12):1459-1463.
[11] 曹雪涛.医学免疫学[M].7版.北京:人民卫生出版社,2018:57-69
[12] 李天星.现代临床免疫学检验[M].北京:军事医学科学出版社,2014:115-128.
[13] 周方元,李艳.乳脂肪球表皮生长因子Ⅷ在冠心病中的作用[J].微循环学杂志,2015,25(4):77-79.
[14] 焦锦玉. MFG-E8调控NETs释放在糖尿病皮损愈合中的作用及其机制研究[D].华中科技大学,2017:22-29.
[15] 罗志红,杨璐璐,艾健,等.复方龙血竭愈伤便捷湿巾治疗军事训练致肢体软组织扭挫伤的临床疗效观察[J].时珍国医国药,2019,30(6):1412-1414.
[16] 刘双苔,陈小艳,翁鸿,等.龙血竭胶囊粉外敷治疗压疮疗效的Meta分析[J].中华现代护理杂志,2017,23(23):3016-3024.
[17] 张平.龙血竭胶囊治疗褥疮临床观察[J].实用中医药杂志,2014,30(6):554.
[18] 周伶俐,黄成坷,林祥杰,等.龙血竭促进糖尿病溃疡大鼠创面修复的机制研究[J].中国临床药理学杂志,2019,35(18):2157-2160.
[19] 张韶湘,余晓玲,邹昱蕾.复方龙血竭口腔溃疡双层膜的研制[J].中国医院药学杂志,2017,37(4):322-324.
[20] 石聪,张兰春,余晓玲,等.龙血竭凝胶剂的制备及对创面组织的修复作用[J].中国实验方剂学杂志,2015,21(2):156-160.

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备注/Memo

备注/Memo:
[基金项目] 云南省科技厅应用基础研究-昆明医科大学联合专项(2017FE467〔-026〕、2018FE001〔-012〕); 云南省大学生创新创业计划项目(201810678007) [作者单位] 1.昆明医科大学生物医学工程研究中心,云南 昆明 650500; 2.云南中医药大学药学院,云南 昆明 650500; 3.昆明学院,云南昆明 6502144; 4. 昆明医科大学科技成果孵化中心电镜室,云南 昆明 650500 [通信作者] 唐莹,E-mail:kmsophie@qq.com
更新日期/Last Update: 2021-01-10