[1]苗颖颖,骆丹.中波紫外线对人真皮成纤维细胞中长链非编码RNA表达的影响[J].中国皮肤性病学杂志,2021,(01):15-7805a2a3-6173-455d-b1f6-9357256070a9/pdf.[doi:10.13735/j.cjdv.1001-7089.202006105]
　MIAO Yingying,LUO Dan.The Effect of UVB Radiation on Expression of Long non-coding RNAs in Human Dermal Fibroblasts[J].The Chinese Journal of Dermatovenereology,2021,(01):15-7805a2a3-6173-455d-b1f6-9357256070a9/pdf.[doi:10.13735/j.cjdv.1001-7089.202006105]
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中波紫外线对人真皮成纤维细胞中长链非编码RNA表达的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年01期
页码:
15-7805a2a3-6173-455d-b1f6-9357256070a9/pdf
栏目:
论著
出版日期:
2021-01-01

文章信息/Info

Title:
The Effect of UVB Radiation on Expression of Long non-coding RNAs in Human Dermal Fibroblasts
文章编号:
1001-7089(2021)01-0015-07
作者:
苗颖颖1; 骆丹2
1.杭州市儿童医院皮肤科,浙江杭州310000;2.南京医科大学附属第一人民医院皮肤科,江苏南京210029
Author(s):
MIAO Yingying1; LUO Dan2
1.Department of Dermatology,Hangzhou Children's Hospital,Hangzhou 310000,China; 2.Department of Dermatology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China
关键词:
中波紫外线;  人真皮成纤维细胞;  长链非编码RNA;  皮肤老化
Keywords:
UVB;  Human dermal fibroblasts;  Long non-coding RNAs;  Skin aging
分类号:
R 751
DOI:
10.13735/j.cjdv.1001-7089.202006105
文献标志码:
A
摘要:
目的 对中波紫外线(ultraviolet rays B,UVB)照射后人真皮成纤维细胞(human dermal fibroblasts,HDFs)中差异表达的长链非编码RNA(long non-ucoding RNAs,LncRNA)进行筛选和功能分析。方法 将处于对数生长期8～11代的HDFs分为对照组和UVB诱导提前老化组(UVB-stress induced premature senescence,UVB-SIPS),用亚毒性剂量(10 mJ/cm2,5次)的UVB构建UVB-SIPS模型,使用CCK-8测定两组HDFs的增殖情况; 用TRIzol提取各组HDFs的RNAs,以|log2(Ratio)| ≥2为标准筛选UVB处理的HDFs中差异表达的lncRNA和mRNA,并对相关的靶基因行生物学分析。结果 以亚毒性剂量的UVB照射后,HDFs的增殖活性明显降低(P<0.05)。设定差异倍数(fold change,FC)≥2时,UVB诱导后的HDFs中检测出差异表达的lncRNAs和mRNAs共4 854个,其中lncRNAs及mRNAs数目分别是3 224和1 630,生物信息学分析提示,差异表达的lncRNA与多种生物学功能有关,且部分涉及光老化。结论 亚毒性剂量的UVB照射可以使HDFs中lncRNAs的表达水平发生明显变化; 这些改变可能与包括皮肤老化在内的生物学功能相关。
Abstract:
Objective To extract the differently expressed long non-coding RNAs(LncRNAs)in Human dermal fibroblasts(HDFs)with UVB radiation,and conduct a screening and functional analysis. Methods The HDFs were selected from 8 to 11 generations in the logarithmic growth phase and were divided into control group and UVB-stress induced premature senescence(UVB-SIPS)group. The UVB-SIPS group was irradiated with subtoxic doses of UVB(10 mJ / cm2 ,5 times).Then CCK-8 was used to detect proliferation activity of HDFs. The total RNAs of each group of HDFs were extracted using TRIzol and analyzed by RNA chip. Normalized intensity | log2(Ratio)| ≥2 was set to find the differently expressed genes in each groups. And the differentially expressed genes were analyzed by GO analysis and pathway enrichment analysis. Results The ability of proliferation of HDFs was significantly lower after exposure to UVB at subtoxic doses than that of the control group( P <0.05). When the set the fold change(FC)≥2,a total number of 4 854 differently expressed lncRNA and mRNA was detected in UVB-induced HDFs,of which the number of lncRNA and mRNA was 3 224 and 1 630 separately. Bioinformatics analysis suggests that differentially expressed lncRNAs involve a variety of biological functions,and some biological functions are related to photoaging. Conclusion Subtoxic dose of UVB irradiation can significantly alter the expression of lncRNAs and mRNAs in HDFs. These alterations are related to various biological phenomena including skin aging.

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备注/Memo

备注/Memo:
[通信作者] 骆丹,E-mail:daniluo2005@163.com
更新日期/Last Update: 2021-01-10