[1]彭亚婷,熊一峰,王淑华,等.miR-186-5p通过抑制Twist1诱导人皮肤成纤维细胞衰老[J].中国皮肤性病学杂志,2021,(01):7-50dc415a-406b-4e30-9e18-2761401da8f7/pdf.[doi:10.13735/j.cjdv.1001-7089.202006052]
　PENG Yating,XIONG Yifeng,WANG Shuhua,et al.miR-186-5p Induces Senescence in Human Skin Fibroblasts by Inhibiting Twist1[J].The Chinese Journal of Dermatovenereology,2021,(01):7-50dc415a-406b-4e30-9e18-2761401da8f7/pdf.[doi:10.13735/j.cjdv.1001-7089.202006052]
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miR-186-5p通过抑制Twist1诱导人皮肤成纤维细胞衰老
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2021年01期
页码:
7-50dc415a-406b-4e30-9e18-2761401da8f7/pdf
栏目:
论著
出版日期:
2021-01-01

文章信息/Info

Title:
miR-186-5p Induces Senescence in Human Skin Fibroblasts by Inhibiting Twist1
文章编号:
1001-7089(2021)01-0007-08
作者:
彭亚婷1; 熊一峰2; 王淑华1; 刘志刚1; 张志彬1; 张颖鹏1
1.南昌大学第二附属医院皮肤科,江西南昌330006;2.南昌大学第一附属医院病理科,江西南昌330006
Author(s):
PENG Yating1; XIONG Yifeng2; WANG Shuhua1; LIU Zhigang 1; ZHANG Zhibing 1; ZHANG Ying peng1
1.Department of Dermatology,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China; 2.Department of Pathology,the First Affiliated Hospital of Nanchang University,Nanchang 330006,China
关键词:
miRNA-186;  microRNA;  Twist;  衰老;  皮肤
Keywords:
miRNA-186;  microRNA;  Twist;  Senescence;  Skin
分类号:
R 751
DOI:
10.13735/j.cjdv.1001-7089.202006052
文献标志码:
A
摘要:
目的 探讨miR-186-5p对人皮肤成纤维细胞衰老的影响及其机制。方法 采用实时定量PCR方法检测miR-186-5p的表达,应用衰老相关β-半乳糖苷酶(SA-β-gal)染色、CCK-8和EdU等方法研究miR-186-5p对人真皮成纤维细胞(HDFs)衰老的作用。通过生物信息学分析、荧光素酶报告试验、实时定量PCR、Western blot和免疫组化法证实miR-186-5p的下游靶基因。结果 首次发现miR-186-5p在传代衰老的HDFs和老年人皮肤真皮组织中表达上调。miR-186-5p的过表达抑制了HDFs细胞的增殖,同时增强了衰老相关指标p16、p21和SA-β-gal的表达。双荧光素酶报告分析显示Twist1是miR-186-5p的下游靶基因。结论 miR-186-5p可通过抑制Twist1诱导HDFs衰老,其可能成为干预皮肤老化的潜在靶点。
Abstract:
Objective To investigate the role of miR-186-5p on the aging of human dermal fibroblasts and its mechanism. Methods The expression of miR-186-5p was measured by quantitative real-time PCR(qRT-PCR). Senescence-associated β-galactosidase(SA-β-gal)staining,the cell counting Kit-8(CCK-8)and 5-ethynyl-2'-deoxyuridine(EdU)were used to investigate the effects of miR-186-5p on human dermal fibroblast(HDF)senescence. The target gene of miR-186-5p was confirmed by bioinformatics analysis,luciferase reporter assay,qRT-PCR,Western blot and immunohistochemical analysis. Results We first found that miR-186-5p was up-regulated in passage-aged HDFs(pHDFs)and skin dermal tissues from old donors(old skin). Overexpression of miR-186-5p inhibited HDFs proliferation while enhanced the expression of p16,p21 and the SA-β-gal-positive ratio. Dual-luciferase reporter assays showed that Twist1 was the downstream target gene of miR-186-5p. Conclusion miR-186-5p could induce HDF senescence by inhibiting Twist1. It could be a potential therapeutic target for preventing skin aging.

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备注/Memo

备注/Memo:
[基金项目] 国家自然科学基金(81860548); 江西省教育厅科学基金(GJJ170146) [通信作者] 张颖鹏,E-mail:48935513@qq.com
更新日期/Last Update: 2021-01-10