[1]薛璐,侯淑萍,单士军,等.特应性皮炎小鼠模型VDRAs对TLR2,TLR4及CRAMP的调控作用[J].中国皮肤性病学杂志,2017,(04):369-374.[doi:10.13735/j.cjdv.1001-7089.201609103]
 XUE Lu,HOU Shu-ping,SHAN Shi-jun,et al.Regulation of TLR2, TLR4 and CRAMP with VDRAs in Atopic Dermatitis Model Mice[J].The Chinese Journal of Dermatovenereology,2017,(04):369-374.[doi:10.13735/j.cjdv.1001-7089.201609103]
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特应性皮炎小鼠模型VDRAs对TLR2,TLR4及CRAMP的调控作用
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2017年04期
页码:
369-374
栏目:
论著
出版日期:
2017-04-01

文章信息/Info

Title:
Regulation of TLR2, TLR4 and CRAMP with VDRAs in Atopic Dermatitis Model Mice
作者:
薛璐 侯淑萍 单士军 罗素菊 岑醒洪 王迪 刘原君 王惠平
天津医科大学总医院皮肤性病科,天津 300052
Author(s):
XUE LuHOU Shu-pingSHAN Shi-junLUO Su-juCEN Xing-hongWANG DiLIU Yuan-junWANG Hui-ping
Department of Dermatology and Venereology,Tianjin Medical University General Hospital,Tianjin 300052,China
关键词:
特应性皮炎 维生素受体激动剂 Toll受体2 Toll受体4 鼠阳离子相关抗菌肽
Keywords:
Atopic dermatitis Vitamin D receptor activators TLR2 TLR4 CRAMP
分类号:
R 758.2
DOI:
10.13735/j.cjdv.1001-7089.201609103
文献标志码:
A
摘要:
目的 观察在特应性皮炎(AD)小鼠模型中维生素D受体激动剂(VDRAs)的干预对皮损以及Toll受体2,4(TLR2,TLR4)和鼠阳离子相关抗菌肽(CRAMP)的影响,并探讨其相互关联机制。方法 将25只雌性BALB / c 小鼠随机分为5组,分别为空白组、AD模型组、AD模型不干预组、PBS对照组、VDRAs治疗组,每组各5只。除空白组以外的其余4组,以二硝基氯苯(DNCB)诱导小鼠特应性皮炎模型。造模成功后,模型组直接处死,AD模型不干预组造模结束后不予以任何处理,VDRAs治疗组腹腔注射VDRAs,PBS对照组腹腔注射PBS液,两组均为每3日1次,总计5次。肉眼观察小鼠背部皮损变化,组织病理观察皮损厚度及炎细胞浸润情况。免疫组化观察各组皮损处TLR2,TLR4及CRAMP的表达部位及水平。Real-Time PCR测各组皮损中TLR2,TLR4及CRAMP的RNA含量,定量分析其变化。结果 VDRAs治疗组小鼠背部皮损炎症反应较AD模型不干预组和PBS对照组明显改善,免疫组化和Real-Time PCR示CRAMP的表达高于AD模型不干预组和PBS对照组,TLR2,TLR4表达较PBS对照组和AD模型组低,差异有统计学意义(P<0.01)。结论 在AD小鼠模型中,VDRAs抑制TLR2,TLR4表达,上调抗菌肽CRAMP的表达。
Abstract:
Objective To detect the effect of vitamin D receptor agonist(VDRAs)on skin lesions improvement and the expression level of toll receptor 2,4(TLR2, TLR4), the antimicrobial peptide CRAMP in atopic dermatitis(AD)model mice, and to explore their inter-related mechanisms. Methods Twenty-five female BABL/c mice were randomly and equally divided into 5 groups: control group, AD model group, AD model no processing group, PBS-control group, VDRAs intervention group. Five mice in each group. The atopic dermatitis(AD)model mice were induced by dinitrochlorobenzene(DNCB)except the control group. After modeling, the AD group were sacrificed. The PBS-control group received intraperitoneal injection of PBS, the VDRAs group received intraperitoneal injection of VDRAs, once every three days, total 5 times. At the end of treatment, the changes of the skin lesions and pathologic condition of the five group mice were detected. The expression and the localization of TLR2, TLR4 and CRAMP in skin lesions were detected through immunohistochemical staining. Real-Time PCR were performed to detect the mRNA expression level of TLR2, TLR4 and CRAMP in serum. Results After treatment with VDRAs, the inflammatory reaction show significantly improvement and the expression level of CRAMP mRNA in serum was increased in VDRAs treatment group compared with the AD model no processing group and the PBS-control. In contrast, the expression level of TLR2 and TLR4 mRNA were decreased compared with the AD model no processing group and the PBS-control(P<0.01). Conclusion In the AD model mice, VDRAs can down-regulate the expression level of TLR2, TLR4 and up-regulate the expression level of antimicrobial peptides CRAMP.

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备注/Memo

备注/Memo:
[基金项目] 国家自然科学基金青年科学基金项目(81301469)
[通讯作者] 王惠平,E-mail:huiping1208@163.com
更新日期/Last Update: 2017-04-05