[1]董颖颖,钟世玉,王琼,等.银屑病小鼠模型建立的新方法[J].中国皮肤性病学杂志,2016,(11):1127-1131.[doi:10.13735/j.cjdv.1001-7089.201605057]
　DONG Ying-ying,ZHONG Shi-yu,WANG Qiong,et al.A Novel Method for Constructing Psoriatic Model in BALB/c Mice[J].The Chinese Journal of Dermatovenereology,2016,(11):1127-1131.[doi:10.13735/j.cjdv.1001-7089.201605057]
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银屑病小鼠模型建立的新方法
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2016年11期
页码:
1127-1131
栏目:
论著
出版日期:
2016-11-01

文章信息/Info

Title:
A Novel Method for Constructing Psoriatic Model in BALB/c Mice
作者:
董颖颖1; 钟世玉1; 王琼1; 吴艳红1; 王秀莹1; 崔鹿1; 安金刚1; 孔刚玉2
1.西安交通大学第二附属医院皮肤科,陕西 西安 710004; 2.西安交通大学电气工程学院,陕西 西安 710049
Author(s):
DONG Ying-ying1; ZHONG Shi-yu1; WANG Qiong1; WU Yan-hong1; WANG Xiu-ying1; CUI Lu1; AN Jin-gang1; KONG Gang-yu2
1.Department of Dermatology,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China; 2.School of Electrical Engineering,Xi'an Jiaotong University,Xi'an 710049,China
关键词:
银屑病模型;  咪喹莫特;  干扰素
Keywords:
Psoriatic model;  Imiquimod;  Interferon
分类号:
R 758.63
DOI:
10.13735/j.cjdv.1001-7089.201605057
文献标志码:
A
摘要:
目的 确立一种新的、有效的、稳定的银屑病鼠造模新方法。方法 使用咪喹莫特乳膏外用及干扰素腹腔内注射造模; 观察BALB/c小鼠皮损改变及稳定性; 并进行皮肤病理片制作和皮损组织病理评分; 免疫组织化学法检测银屑病样小鼠皮损中CD3+,CD4+,CD8+T细胞、IL-12,IL-17,IL-23,TNF-α,MPO及SOD的表达情况; 与正常组小鼠比对,观察造模效果。结果 肉眼观察模型小鼠皮肤较对照组明显增厚,颜色偏红,表面覆盖厚层鳞屑,符合银屑病样改变; 银屑病组织病理学Baker评分符合银屑病样改变; 银屑病样模型小鼠皮肤中CD3+,CD4+及CD8+T细胞在真皮层较正常鼠显著增多,两组比对差异有统计学意义; IL-12,IL-17,IL-23及TNF-α银屑病样模型小鼠皮肤中表达增强,与正常组相比差异有统计学意义; 模型组小鼠皮损MPO表达较正常小鼠显著增多。模型组小鼠皮损中SOD表达减少; 结果显示造模小鼠皮损符合银屑病改变,皮损典型,2周内皮损未消退。结论 该方法是一种有效的、稳定的、值得推广的银屑病造模新方法。
Abstract:
Objective To find a novel method for constructing murine psoriatic model with high efficiency and stability.Methods BALB/c mice were treated with topical imiquimod application or in combination with intraperitoneal injection of interferon. The skin lesion was observed for both changes and stability. After section preparation and histological evaluation of skin lesion, immunohistochemistry was performed to assess the expression of CD3, CD4, CD8, IL-12, IL-17, IL-23, TNF-α, MPO, and SOD. These mice were compared to normal individuals.Results By optical observation, the treated mice showed thicker more reddish skin than the normal controls. The lesional skin was covered with scales, similar to psoriatic changes. Histological analysis confirmed typical psoriasis-like features by Baker scoring. Moreover, the treatment group had more CD3+/CD4+/CD8+ T cells in dermis than the controls(P< 0.05). Similar results were also seen with IL-12, IL-17, IL-23, and TNF-α assays. Besides, the treated mice exhibited more MPO expression than control mice(P< 0.05). Relatively, the former mice had less SOD level in the lesion. The cutaneous lesion persisted in the treated mice during the two-week follow-up.Conclusion The combination of imiquimod and interferon treatments induces typical and stable psoriasis-like lesion in mice, and deserves extensive application.

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备注/Memo

备注/Memo:
[基金项目] 西安交通大学千人计划项目(No.2014-09); 国家自然科学基金资助项目(No.81601630); 陕西省自然科学基金青年人才项目(No.2016JQ8044)
更新日期/Last Update: 2016-11-01