[1]王鹏,冯燕艳,张泽高,等.维吾尔族Kaposi's肉瘤患者组织中KSHV-vFLIP表达水平与患者临床特征相关性[J].中国皮肤性病学杂志,2016,(11):1120-1122,1191.[doi:10.13735/j.cjdv.1001-7089.201606089]
 WANG Peng,FENG Yan-yan,ZHANG Ze-gao,et al.KSHV-vFLIP Expression and Its Relationship with Disease of Different Lesion Types in Patients with Kaposi's Sarcoma[J].The Chinese Journal of Dermatovenereology,2016,(11):1120-1122,1191.[doi:10.13735/j.cjdv.1001-7089.201606089]
点击复制

维吾尔族Kaposi's肉瘤患者组织中KSHV-vFLIP表达水平与患者临床特征相关性
分享到:

《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2016年11期
页码:
1120-1122,1191
栏目:
论著
出版日期:
2016-11-01

文章信息/Info

Title:
KSHV-vFLIP Expression and Its Relationship with Disease of Different Lesion Types in Patients with Kaposi's Sarcoma
作者:
王鹏1冯燕艳1张泽高2马云霞1
1.新疆维吾尔自治区人民医院皮肤科,新疆 乌鲁木齐 830000; 2.新疆维吾尔自治区人民医院放疗二科,新疆 乌鲁木齐 830000
Author(s):
WANG Peng1FENG Yan-yan1ZHANG Ze-gao2MA Yun-xia1
1. Department of Dermatology, People's Hospital of Xinjiang Uygur Autonomous Region, Uygur 830000, China; 2. Radiotherapy Department 2, People's Hospital of Xinjiang Uygur Autonomous Region, Uygur 830000, China
关键词:
卡波西肉瘤 KSHV-vFLIP基因 实时荧光定量 石蜡包埋 瘤体体积
Keywords:
Kaposi's sarcoma Kaposi's sarcoma herpesvirus-FADD-like interleukin-1-b-converting enzyme(FLICE)/caspase8 inhibitory protein(KSHV-vFLIP) Reverse transcription(RT)-PCR Paraffin-embedded Tumor's volume.
分类号:
R 739.5
DOI:
10.13735/j.cjdv.1001-7089.201606089
文献标志码:
A
摘要:
目的 探讨维吾尔族卡波西肉瘤(Kaposi's sarcoma,KS)患者皮损组织内KSHV-vFLIP基因表达水平与临床特征的相关性。 方法 选取29例维吾尔族卡波西肉瘤患者和12例血管瘤(hemangioma)患者、11例健康对照患者皮损石蜡包埋组织,反转录-聚合酶链反应(RT-PCR)分析KSHV-vFLIP基因mRNA表达水平。结果 KS患者KSHV-vFLIP基因表达水平(4.436±0.508)显著高于健康对照组(1.145±0.112,P<0.05),其中斑块型组KSHV-vFLIP基因表达水平(8.104±2.225)显著高于结节型组(3.971±0.371, P<0.05)和斑片组(2.774±0.867, P<0.05)。而KS组(4.436±0.507)与血管瘤组(6.343±0.949)差异无统计学意义(P>0.05),且HIV携带组(3.739±0.816)与非HIV携带组(4.618±0.604)差异无统计学意义(P>0.05)。结论 在维吾尔族卡波西肉瘤患者组织中,KSHV-vFLIP基因表达水平与瘤体体积正相关。
Abstract:
Objective To investigate KSHV-vFLIP gene expression in skin lesions from uygur patients with Kaposi's Sarcoma(KS), and to explore its relationship with Clinical Feature.Methods Paraffin -embedded tissues were obtained from 29 patients with KS and 12 patients with Hemangioma, and collected 11 healthy human as control. Reverse transcription(RT)-PCR was performed to detect the mRNA expression of KSHV-vFLIP in lesions.Results Compared with healthy controls, 29 patients with KS showed significantly decreased KSHV-vFLIP mRNA expression levels(4.436±0.508 vs.1.145±0.112, P<0.05 ). Patients with KS of plaque type showed enhanced KSHV-vFLIP mRNA expression levels compared with patients with KS of nodule type and patch type(8.104±2.225 vs. 3.971±0.371, 2.774±0.867, P<0.05). However, there were no significant difference between 29 patients with KS and 12 patients with Hemangioma(4.436±0.508 vs.6.343±0.949, P>0.05). And, there were no significant difference between 6 patients with KS tested HIV+ and 23 patients with KS tested HIV-(4.436±0.508vs.6.343±0.949,P>0.05).Conclusion There was a positive correlation between the KSHV-vFLIP gene expression and tumor's volume in lesion from uygur patients with Kaposi's sarcoma(KS).

参考文献/References:

[1] 吴秀娟. 卡波西肉瘤miRNA差异表达谱的筛选及miR-126-3p对卡波西肉瘤细胞株SLK生物学性状的影响及靶基因研究[D].安徽医科大学,2015.
[2] 沈大为,石得仁,普雄明.经典型Kaposi's肉瘤(23例分析)[J].临床皮肤科杂志, 1993, 22(3): 136-138.
[3] Chang Y, Cesarman E, Pessin MS,et al. Identification of herpesvirus like DNA sequences in AIDS-associated Kaposi's sarcoma [J]. Science, 1994, 266(5192): 1865-1869.
[4] Shimizu A, Baratchian M, Takeuchi Y,et al. Kaposi's sarcoma-associated herpesvirus vFLIP and human T cell lymphotropic virus type 1 Tax oncogenic proteins activate kappaB kinase subunit gamma by different mechanisms independent of the physiological cytokine-induced pathways[J]. J Virol, 2011, 85(14): 7444-7448.
[5] Sturzl M, Hohenadl C, Zietz C, et al. Expression of K13/v-FLIP gene of human herpesvirus 8 and apoptosis in Kaposi's sarcoma spindle cells [J]. J Natl Cancer Inst, 1999, 91(20):1725-1733.
[6] Nayar U, Lu P, Goldstein RL,et al. Targeting the Hsp90-associated viral oncoproteome in gamma herpesvirus-associated malignancies [J]. Blood, 2013, 122(16): 2837-2847.
[7] Ballon G, Akar G, Cesarman E. Systemic Expression of Kaposi Sarcoma Herpesvirus(KSHV)Vflip in Endothelial Cells Leads to a Profound Proinflammatory Phenotype and Myeloid Lineage RemodelingIn Vivo[J].PLoS Pathogens, 2015, 11(1):e1004581.
[8] Moujalled DM, Cook WD, Lluis JM, et al. In mouse embryonic fibroblasts, neither caspase-8 nor cellular FLICE-inhibitory protein(FLIP)is necessary for TNF to activateNF-κB, but caspase-8 is required for TNF to cause cell death, and induction of FLIP by NF-κB is required to prevent it[J]. Cell Death and Differentiation, 2012, 19(5):808-815.
[9] Matta H, Chaudhary PM. Activation of alternative NF-κB pathway by human herpes virus 8-encoded Fas-associated death domain-like IL-1β-converting enzyme inhibitory protein(vFLIP)[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004,101(25):9399-9404.
[10] Suthaus J, Stuhlmann-Laeisz C, Tompkins VS, et al. HHV-8–encoded viral IL-6 collaborates with mouse IL-6 in the development of multicentric Castleman disease in mice[J]. Blood, 2012, 119(22):5173-5181.
[11] Sakakibara S, Espigol-Frigole G, Gasperini P, et al. A20/TNFAIP3 inhibits NF-κB activation induced by the Kaposi's sarcoma-associated herpesvirus vFLIP oncoprotein [J]. Oncogene, 2013, 32(10):1223-1232.
[12] Alkharsah KR, Singh VV, Bosco R, et al. Deletion of Kaposi's Sarcoma-Associated Herpesvirus FLICE Inhibitory Protein, vFLIP, from the Viral Genome Compromises the Activation of STAT1-Responsive Cellular Genes and Spindle Cell Formation in Endothelial Cells [J]. Journal of Virology, 2011, 85(19):10375-10388.

相似文献/References:

[1]赵云,李露,余德厚,等.汉族艾滋病相关卡波西肉瘤1例[J].中国皮肤性病学杂志,2016,(02):173.[doi:10.13735/j.cjdv.1001-7089.201505152]
 ZHAO Yun,LI Lu,YU De hou,et al.A Case of AIDSrelated Kaposi's Sarcoma in a Han Chinese[J].The Chinese Journal of Dermatovenereology,2016,(11):173.[doi:10.13735/j.cjdv.1001-7089.201505152]
[2]翁文佳,李娟,宋冰冰,等.误诊为疖的艾滋病相关Kaposi肉瘤1例[J].中国皮肤性病学杂志,2019,(05):604.[doi:10.13735/j.cjdv.1001-7089.201801074]
 WENG Wenqian,LI Juan,SONG Bingbing,et al.A Case of HIV-related Kaposi's Sarcoma Misdiagnosed as Furuncle[J].The Chinese Journal of Dermatovenereology,2019,(11):604.[doi:10.13735/j.cjdv.1001-7089.201801074]

备注/Memo

备注/Memo:
[基金项目] 新疆维吾尔自治区人民医院院内科研项目(20130303)
更新日期/Last Update: 2016-11-01