[1]李彤,卢浩,陈际名,等.表没食子儿茶素没食子酸酯对紫外线损伤小鼠皮肤的保护作用[J].中国皮肤性病学杂志,2016,(11):1107-1111.[doi:10.13735/j.cjdv.1001-7089.201603003]
 LI Tong,LU Hao,CHEN Ji-ming,et al.Effect of EGCG on Protection of Mice Skin Injury Induced by Ultraviolet A and Ultraviolet B[J].The Chinese Journal of Dermatovenereology,2016,(11):1107-1111.[doi:10.13735/j.cjdv.1001-7089.201603003]
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表没食子儿茶素没食子酸酯对紫外线损伤小鼠皮肤的保护作用
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2016年11期
页码:
1107-1111
栏目:
论著
出版日期:
2016-11-01

文章信息/Info

Title:
Effect of EGCG on Protection of Mice Skin Injury Induced by Ultraviolet A and Ultraviolet B
作者:
李彤12卢浩12陈际名12魏楠12李亦凡12王宣军12马啸12
1.云南农业大学普洱茶学教育部重点实验室,云南 昆明 650201; 2.云南省茶深加工工程技术研究中心,云南 昆明 650201
Author(s):
LI Tong12LU Hao12CHEN Ji-ming12WEI Nan12LI Yi-fan12WANG Xuan-jun12MA Xiao12
1.Key Laboratory of Puer Tea Science,Ministry of Education,Yunnan Agriculture University, Kunming 650201,China; 2.Tea Research Center of Yunnan, Kunming 650201,China
关键词:
紫外线 表没食子儿茶素没食子酸酯 氧化应激 凋亡蛋白
Keywords:
Ultraviolet EGCG Oxidative stress Apoptotic proteins
分类号:
R 758.1
DOI:
10.13735/j.cjdv.1001-7089.201603003
文献标志码:
A
摘要:
目的 探讨表没食子儿茶素没食子酸酯(EGCG)对紫外线UVA,UVB辐照引起的皮肤损伤的保护作用。方法 将36只雄性BALB/c小鼠随机分成6组,用UVA,UVB紫外灯管照射小鼠背部脱毛后的皮肤,诱导皮肤损伤模型建立。在当天照射结束后半小时之内进行相应的药物涂抹,最后一次照射结束后24h取小鼠背部皮肤,组织病理学切片、染色后镜下观察其组织形态变化; 氧化还原试剂盒测定氧化应激程度; 蛋白免疫印迹法检测凋亡蛋白表达量。结果 5%EGCG组和10%EGCG组与模型组相比,小鼠皮肤状态明显改善,干燥、红肿状态减轻,皮肤组织中胶原蛋白含量高于模型组,氧化应激反应低于模型组,凋亡蛋白表达量低于模型组,差异均有统计学意义(P<0.05)。结论 EGCG可以保护因紫外线辐照而受损的皮肤。
Abstract:
Objective To study the protection of EGCG on skin damage induced by ultraviolet A and ultraviolet B.Methods Thirty-six male BALB/c mice were randomly divided into 6 groups of 6 animals. Animals were exposed to ultraviolet A and ultraviolet B generated by special lamps. After the irradiation,apply all the stuff topically to each group in 30 minites. 24 hours after the last irradiation, the full-thickness skin samples were removed respectively, the skin histochemistry was detected by H&E and Masson staining. The skin oxidative stress was detected by redox kits. The expression of apoptotic proteins was detected by Western Blotting.Results The conditions of the skin of 5% EGCG-treatment group and 10% EGCG-treatment group were improved, the levels of collagen were higher than UV groups, and the levels of oxidative stress and the expression of apoptotic proteins were decreased than UV groups(P<0.05)after EGCG treatment.Conclusion EGCG can protect the skin from UV radiation.

参考文献/References:

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更新日期/Last Update: 2016-11-01