[1]韩露露,钱革,胡桂兰,等.UVB照射对系统性红斑狼疮自噬相关基因及蛋白表达水平的影响[J].中国皮肤性病学杂志,2016,(05):445-448.[doi:10.13735/j.cjdv.1001-7089.201506182]
　HAN Lu lu,QIAN Ge,HU Gui lan,et al.The Influence of UVB Irradiation on the Expression Levels of Autophagy Related Proteins and Genes in Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2016,(05):445-448.[doi:10.13735/j.cjdv.1001-7089.201506182]
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UVB照射对系统性红斑狼疮自噬相关基因及蛋白表达水平的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2016年05期
页码:
445-448
栏目:
论著
出版日期:
2016-05-01

文章信息/Info

Title:
The Influence of UVB Irradiation on the Expression Levels of Autophagy Related Proteins and Genes in Systemic Lupus Erythematosus
作者:
韩露露1; 钱革2; 胡桂兰1; 邓丹琪1
1昆明医科大学第二附属医院皮肤性病科，云南 昆明650101；2郑州市儿童医院，河南 郑州 450000
Author(s):
HAN Lulu1; QIAN Ge2; HU Guilan1; DENG Danqi1
1Department of Dermatology,the Second Affiliated Hospital of Kunming Medical University,Kunming 650101,China;2Department of Dermatology,Zhengzhou Children's Hospital,Zhengzhou 450000,China
关键词:
系统性红斑狼疮; UVB; 自噬; 单个核细胞
Keywords:
Systemic lupus erythematosus; UVB; Autophagy; Mononuclear cell
分类号:
R 59324
DOI:
10.13735/j.cjdv.1001-7089.201506182
文献标志码:
A
摘要:
目的 观察UVB照射对SLE患者的外周血单个核细胞(PBMC)的自噬现象，探讨UVB、自噬对SLE产生的影响。方法 应用RTqPCR，Western blotting技术在基因和蛋白水平测定活动性SLE患者外周血淋巴细胞自噬标志物Uvrag，Beclin1和LC3Ⅱ的表达水平，并通过统计学分析其内在联系，探讨自噬在SLE发病中的作用机制。结果 RTqPCR显示：SLE患者外周血单个核细胞接受UVB照射后Uvrag，Beclin1和LC3Ⅱ mRNA的表达水平较照射前明显升高(P<005)；SLE患者外周血单个核细胞接受UVB照射后LC3ⅡmRNA的表达水平明显高于正常对照组接受UVB照射的水平(P<001)。Western blotting显示：SLE患者的外周血单个核细胞接受UVB照射组较SLE患者组的外周血单个核细胞中的Uvrag,Beclin1和LC3Ⅱ的蛋白水平升高，Uvrag,Beclin1差异有统计学意义(P<005)；SLE患者的外周血单个核细胞接受UVB照射组较正常人的外周血单个核细胞接受UVB照射组的Uvrag，Beclin1和LC3Ⅱ的蛋白水平轻度升高，LC3Ⅱ差异有统计学意义(P<005)；SLE患者的外周血单个核细胞在接受UVB照射组较正常未照射UVB组的外周血单个核细胞中的Uvrag，Beclin1和LC3Ⅱ蛋白水平升高(P<005)。结论 活动性SLE患者外周血单个核细胞中存在自噬现象，UVB能够引发SLE患者发生异常自噬，自噬相关基因/蛋白参与了SLE外周血单个核细胞异常自噬的发生。
Abstract:
Objective To study the effects of UVB irradiation on autophagy in peripheral blood mononuclear cells of SLE patients,and to reveal the impacts of UVB irradiation and autophagy on SLE Methods RTqPCR and Western blotting techniques were used to measure the expression levels of the autophagy associated markers (Uvrag,Beclin1 and LC3Ⅱ )in peripheral mononuclear cells of patients with active SLEStatistical analysis was performed to determine pathogenetic role of autophagy in active SLE Results RTqPCR results showed that the expression levels of LC3Ⅱ，Beclin1 and Uvrag mRNA were significantly increased in active SLE patients after UVBexposure compared to before UVB exposure (P<005)Following UVB irradiation,the expression levels of LC3ⅡmRNA were higher in SLE patients than in normal controls (P<001)Moreover,UVB irradiation also significantly increased the expression levels of Beclin1 and Uvrag proteins in SLE patients (P<005)while LC3Ⅱexpression was slightly increasedAfter UVB irradiation,the expression levels of LC3Ⅱexpression were significantly higher in SLE patients than in normal controls (P<005)In comparison with normal controls,the expression levels of LC3 Ⅱ,Beclin1 and Uvrag proteins were significantly higher in patients with active SLE after UVB exposure(P<005) Conclusion Autophagy occurs in peripheral blood mononuclear cells in patients with active SLEUVB irradiation can alter autophagy in patients with active SLEAutophagy related genes and associated proteins are likely involved in the process of autophagy occurring in peripheral blood mononuclear cells of patients with active SLE

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备注/Memo

备注/Memo:
［基金项目］ 国家自然科学基金 (81460472)；云南省科技厅昆明医学院联合专项基金资助项目(2014FB050)
更新日期/Last Update: 2016-05-20