[1]韩露露,钱革,胡桂兰,等.UVB照射对系统性红斑狼疮自噬相关基因及蛋白表达水平的影响[J].中国皮肤性病学杂志,2016,(05):445-448.[doi:10.13735/j.cjdv.1001-7089.201506182]
 HAN Lu lu,QIAN Ge,HU Gui lan,et al.The Influence of UVB Irradiation on the Expression Levels of Autophagy Related Proteins and Genes in Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2016,(05):445-448.[doi:10.13735/j.cjdv.1001-7089.201506182]
点击复制

UVB照射对系统性红斑狼疮自噬相关基因及蛋白表达水平的影响
分享到:

《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2016年05期
页码:
445-448
栏目:
论著
出版日期:
2016-05-01

文章信息/Info

Title:
The Influence of UVB Irradiation on the Expression Levels of Autophagy Related Proteins and Genes in Systemic Lupus Erythematosus
作者:
韩露露1钱革2胡桂兰1邓丹琪1
1昆明医科大学第二附属医院皮肤性病科,云南 昆明650101;2郑州市儿童医院,河南 郑州 450000
Author(s):
HAN Lulu1QIAN Ge2HU Guilan1DENG Danqi1
1Department of Dermatology,the Second Affiliated Hospital of Kunming Medical University,Kunming 650101,China;2Department of Dermatology,Zhengzhou Children's Hospital,Zhengzhou 450000,China
关键词:
系统性红斑狼疮UVB自噬单个核细胞
Keywords:
Systemic lupus erythematosusUVBAutophagyMononuclear cell
分类号:
R 59324
DOI:
10.13735/j.cjdv.1001-7089.201506182
文献标志码:
A
摘要:
目的 观察UVB照射对SLE患者的外周血单个核细胞(PBMC)的自噬现象,探讨UVB、自噬对SLE产生的影响。方法 应用RTqPCR,Western blotting技术在基因和蛋白水平测定活动性SLE患者外周血淋巴细胞自噬标志物Uvrag,Beclin1和LC3Ⅱ的表达水平,并通过统计学分析其内在联系,探讨自噬在SLE发病中的作用机制。结果 RTqPCR显示:SLE患者外周血单个核细胞接受UVB照射后Uvrag,Beclin1和LC3Ⅱ mRNA的表达水平较照射前明显升高(P<005);SLE患者外周血单个核细胞接受UVB照射后LC3ⅡmRNA的表达水平明显高于正常对照组接受UVB照射的水平(P<001)。Western blotting显示:SLE患者的外周血单个核细胞接受UVB照射组较SLE患者组的外周血单个核细胞中的Uvrag,Beclin1和LC3Ⅱ的蛋白水平升高,Uvrag,Beclin1差异有统计学意义(P<005);SLE患者的外周血单个核细胞接受UVB照射组较正常人的外周血单个核细胞接受UVB照射组的Uvrag,Beclin1和LC3Ⅱ的蛋白水平轻度升高,LC3Ⅱ差异有统计学意义(P<005);SLE患者的外周血单个核细胞在接受UVB照射组较正常未照射UVB组的外周血单个核细胞中的Uvrag,Beclin1和LC3Ⅱ蛋白水平升高(P<005)。结论 活动性SLE患者外周血单个核细胞中存在自噬现象,UVB能够引发SLE患者发生异常自噬,自噬相关基因/蛋白参与了SLE外周血单个核细胞异常自噬的发生。
Abstract:
Objective To study the effects of UVB irradiation on autophagy in peripheral blood mononuclear cells of SLE patients,and to reveal the impacts of UVB irradiation and autophagy on SLE Methods RTqPCR and Western blotting techniques were used to measure the expression levels of the autophagy associated markers (Uvrag,Beclin1 and LC3Ⅱ )in peripheral mononuclear cells of patients with active SLEStatistical analysis was performed to determine pathogenetic role of autophagy in active SLE Results RTqPCR results showed that the expression levels of LC3Ⅱ,Beclin1 and Uvrag mRNA were significantly increased in active SLE patients after UVBexposure compared to before UVB exposure (P<005)Following UVB irradiation,the expression levels of LC3ⅡmRNA were higher in SLE patients than in normal controls (P<001)Moreover,UVB irradiation also significantly increased the expression levels of Beclin1 and Uvrag proteins in SLE patients (P<005)while LC3Ⅱexpression was slightly increasedAfter UVB irradiation,the expression levels of LC3Ⅱexpression were significantly higher in SLE patients than in normal controls (P<005)In comparison with normal controls,the expression levels of LC3 Ⅱ,Beclin1 and Uvrag proteins were significantly higher in patients with active SLE after UVB exposure(P<005) Conclusion Autophagy occurs in peripheral blood mononuclear cells in patients with active SLEUVB irradiation can alter autophagy in patients with active SLEAutophagy related genes and associated proteins are likely involved in the process of autophagy occurring in peripheral blood mononuclear cells of patients with active SLE

参考文献/References:

[1] Levine B,Klionsky DJ.Development by selfdigestion:molecular mechanisms and biological functions of autophagy [J].Dev Cell,2004,6(4):463-477. 
[2] Gateva V,Sandling JK,Hom G,et al.A largescale replication study identifies TNIP1,PRDM1,JAZF1,UHRF1BP1 and IL10 as risk loci for systemic lupus erythematosus [J].Nat Genet,2009,41(11):1228-1233. 
[3] 陈旭,张青松,鞠梅,等.中波紫外线诱导人皮肤成纤维细胞自噬对凋亡影响的初步研究[J].中华皮肤科杂志,2012,45(7):496-500. 
[4] International Consortium for Systemic Lupus Erythematosus Genetics (SLEGEN),Harley JB,AlarcónRiquelme ME,et al.Genomewide association scan in women with systemic lupus erythematosus identifies susceptibility variants in ITGAM,PXK,KIAA1542 and other loci [J].Nat Genet,2008,40(2):204-210. 
[5] Zhou XJ,Lu XL,Lv JC,et al.Genetic association of PRDM1ATG5 intergenic region and autophagy with systemic lupus erythematosus in a Chinese population [J].Ann Rheum Dis,2011,70 (7):1330-1337. 
[6] Yang W,Tang H,Zhang Y,et al.Metaanalysis followed by replication identifies loci in or near CDKN1B,TET3,CD80,DRAM1 and ARID5B as associated with systemic lupus erythematosus in Asians [J].Am J Hum Genet,2013,92(1):41-51. 
[7] Gros F,Arnold J,Page N,et al.Macroautophagy is deregulated in murine and human lupus T lymphocytes [J].Autophagy,2012,8(7):1113-1123. 
[8] 张青松,顾恒.UVB对人皮肤成纤维细胞自噬影响的初步研究[J].中国麻风皮肤病杂志,2008,24(7):511-513. 
[9] Yang Y,Wang H,Wang S,et al.GSK3β signaling is involved in ultraviolet Binduced activation of autophagy in epidermal cells [J].Int J Oncol,2012,41(5):1782-1788. 
[10] 张青松,鞠梅,陈崑,等.培养的人皮肤成纤维细胞光老化模型中自噬水平的研究 [J].中华皮肤科杂志,2010,43(8):572-574. 
[11] Koike T.The new era of autoimmune disease research [J].Arthritis Res Ther, 2011,13(3):113. 
[12] DuarteGarcia A,Fang H,To CH,et al.Seasonal variation in the activity of systemic lupus erythematosus [J].J Rheumatol,2012,39(7):1392-1398. 
[13] 陈崑,常宝珠,郑家润.红斑狼疮光敏感性发生机制的研究进展[J].国外医学皮肤性病学分册,2002,28(2):87-90. 
[14] Kuhn A,Ruland V,Bonsmann G.Photosensitivity,phototesting and photoprotection in cutaneous lupus erythematosus [J].Lupus,2010,19(9):1036-1046. 
[15] Wu Z,Li X,Qin H,et al.Ultraviolet B enhances DNA hypomethylation of CD4+ T cells in systemic lupus erythematosus viainhibiting DNMT1 catalytic activity [J].J Dermatol Sci,2013,71(3):167-173. 
[16] Kirou KA,Gkrouzman E.Antiinterferon alpha treatment in SLE [J].Clin Immunol,2013,148(3):303-312. 
[17] Cao Y,Klionsky DJ.Physiological functions of At96/Beclin l:aunique autophagyrelated protein [J].Cell Res,2009,5(7):973-979. 
[18] Kihara A,Kabeya Y,Ohsumi Y,et al.Beclinphosphatidylinositol 3l(inase complex functions at the transGolgi network[J].EMBO Rep,2001,2(4):330-335.
[19] Noboru M.Methods for monitoring autophagy [J].The Int J Biochem Cell Biol,2004,36(12):2491-2502. 
[20] Kabeya Y,Mizushima N,Yamamoto A,et al.LC3,GABARAP and GATEl 6 localize to autophagosomal membrane depending on formII formation [J].J Cell Sci,2004,117(13):2805-2812. 
[21] Alessandri C,Barbati C,Vacirca D,et al.T lymphocytes from patients with systemic lupus erythematosus are resistant to induction of autophagy [J].FASEB J,2012,26(11):4722-4732. 
[22] 赵继军,李美荣,陆才生,等.活动性系统性红斑狼疮自噬及相关基因研究[J].中华风湿病学杂志,2009,13(3):148-151. 
[23] Teitz T.Isolation by polymerase chain reaction of a cDNA whose product partially complements the ultraviolet sensitivity of xeroderma pigmentosum group C ceils [J].Gene,1990,87(2):295-298. 
[24] Liang C.Autophagic and tumour suppressor activity of a novel Beclinlbinding protein UVRAG [J].Nat Cell Biol,2006,8(7):688-699. 
[25] 付俊,尚海旭,贾弘禔,等.Beclin1与自噬及肿瘤的关系[J].生理科学进展,2012,43(2):155-158.

相似文献/References:

[1]王蓉,李桂珍,陈雪雯,等.系统性红斑狼疮患者外周血T淋巴细胞的激活状态[J].中国皮肤性病学杂志,2015,(02):111.[doi:10.13735/j.cjdv.1001-7089.201408034]
 WANG Rong,LI Gui zhen,CHEN Xue wen,et al.T Lymphocytes Activity in Peripheral Blood of Patients with Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2015,(05):111.[doi:10.13735/j.cjdv.1001-7089.201408034]
[2]王倩,刘伟,卢葳,等.系统性红斑狼疮患者血清中趋化因子CXCL9,CXCL10及CXCL11的检测[J].中国皮肤性病学杂志,2015,(02):125.[doi:10.13735/j.cjdv.1001-7089.201405189]
 WANG Qian,LIU Wei,LU Wei,et al.Detection of Chemokine CXCL9,CXCL10 and CXCL11 in the Serum of Patients with Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2015,(05):125.[doi:10.13735/j.cjdv.1001-7089.201405189]
[3]张娜,付学锋,陈筱筱,等.干燥综合征108例临床分析[J].中国皮肤性病学杂志,2015,(04):368.[doi:10.13735/j.cjdv.1001-7089.201408102]
 ZHANG Na,FU Xue feng,CHEN Xiao xiao,et al.Clinical Analysis in 108 Patients with Sjgren′s Syndrome[J].The Chinese Journal of Dermatovenereology,2015,(05):368.[doi:10.13735/j.cjdv.1001-7089.201408102]
[4]周冼苡䥺Symbol`@@,杨烨玫,周思彤.微波联合光动力疗法治疗系统性红斑狼疮并尖锐湿疣1例[J].中国皮肤性病学杂志,2015,(06):608.[doi:10.13735/j.cjdv.1001-7089.201411120]
 ZHOU Xian yi,YANG Ye mei,ZHOU Si tong.Microwave Combined with Photodynamics for Condyloma Acuminatum in Patient with Systemic Lupus Erythematosus Patient: a Case Report[J].The Chinese Journal of Dermatovenereology,2015,(05):608.[doi:10.13735/j.cjdv.1001-7089.201411120]
[5]梅莉红,干慧慧,翁孟武,等.系统性红斑狼疮患者血清中肺炎衣原体感染的检测[J].中国皮肤性病学杂志,2015,(07):670.[doi:10.13735/j.cjdv.1001-7089.201410048]
 MEI Li hong,GAN Hui hui,WENG Meng wu,et al.Detection of Chlamydia Pneumoniae Antibodies in Serum of Patients with Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2015,(05):670.[doi:10.13735/j.cjdv.1001-7089.201410048]
[6]卢井发,叶小英,万春雷,等.系统性红斑狼疮、多发性肌炎和系统性硬皮病重叠综合征1例[J].中国皮肤性病学杂志,2015,(09):941.[doi:10.13735/j.cjdv.1001-7089.201501068]
 LU Jing fa,YE Xiao ying,WAN Chun lei,et al.A Case of Overlap Syndrome with Systemic Lupus Erythematosus,Polymyositis and Systemic Scleroderma[J].The Chinese Journal of Dermatovenereology,2015,(05):941.[doi:10.13735/j.cjdv.1001-7089.201501068]
[7]冉昕,邓丹琪.系统性红斑狼疮与地理环境因素的研究进展[J].中国皮肤性病学杂志,2015,(10):1082.[doi:10.13735/j.cjdv.1001.7089.201408120]
 RAN Xin,DENG Dan qi.Research Progress on Environmental Factors in Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2015,(05):1082.[doi:10.13735/j.cjdv.1001.7089.201408120]
[8]朱小华,梁俊,杨永生,等.SLE患者外周血T淋巴细胞p53基因表达及其与DNA甲基化的相关性[J].中国皮肤性病学杂志,2016,(04):334.[doi:10.13735/j.cjdv.1001-7089.201504065]
 ZHU Xiao hua,LIANG Jun,YANG Yong sheng,et al.Associations Between Expression of p53mRNA and DNA Methylation in T Cells From the Patients With Systemic Lupus Erythematosus[J].The Chinese Journal of Dermatovenereology,2016,(05):334.[doi:10.13735/j.cjdv.1001-7089.201504065]
[9]赵俊芳,李桂珍,李孟娟.SLE患者抗核小体抗体、抗dsDNA抗体及抗C1q抗体的检测[J].中国皮肤性病学杂志,2017,(02):156.[doi:10.13735/j.cjdv.1001-7089.201609041]
 ZHAO Jun-fang,LI Gui-zhen,LI Meng-juan.Detection of Anti-nucleosome Antibody,Anti-C1q Antibody and Anti-dsDNA Antibody in Patients with Systemic Lupus Erythematosus(SLE)[J].The Chinese Journal of Dermatovenereology,2017,(05):156.[doi:10.13735/j.cjdv.1001-7089.201609041]
[10]施银娟,刘璐,林艳,等.系统性红斑狼疮淋巴细胞减少的研究进展[J].中国皮肤性病学杂志,2017,(02):219.[doi:10.13735/j.cjdv.1001-7089.201507004]
 SHI Yin-juan,LIU Lu,LIN Yan,et al.Lymphopenia in Systemic Lupus Erythematosus: Mechanism and Clinical Correlation[J].The Chinese Journal of Dermatovenereology,2017,(05):219.[doi:10.13735/j.cjdv.1001-7089.201507004]

备注/Memo

备注/Memo:
[基金项目] 国家自然科学基金 (81460472);云南省科技厅昆明医学院联合专项基金资助项目(2014FB050)
更新日期/Last Update: 2016-05-20