[1]郝泽芳,潘婧,吴训伟.小鼠皮肤移植细胞有效重建毛发[J].中国皮肤性病学杂志,2015,(11):1130-1133,1144.[doi:10.13735/j.cjdv.1001-7089.201503065]
 HAO Ze fang,PAN Jing,WU Xun wei.A New Method to Efficiently Reconstitute Hair Follicles by Grafting of Isolated Skin Cells[J].The Chinese Journal of Dermatovenereology,2015,(11):1130-1133,1144.[doi:10.13735/j.cjdv.1001-7089.201503065]
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小鼠皮肤移植细胞有效重建毛发
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2015年11期
页码:
1130-1133,1144
栏目:
论著
出版日期:
2015-11-01

文章信息/Info

Title:
A New Method to Efficiently Reconstitute Hair Follicles by Grafting of Isolated Skin Cells
作者:
郝泽芳1潘婧1吴训伟23
1.济南市妇幼保健院妇产科,山东 济南 250001;2.济南磐升生物技术有限公司,山东 济南 250101;3.山东大学口腔医学院,山东 济南 250012
Author(s):
HAO Ze fang 1PAN Jing 1WU Xun wei 23
1Jinan Maternal and child health care hospital,Jinan 250001,China;2Jinan Pantheon ltdco,Jinan 250101,China;3School of Stomatology Shandong University,Jinan 250012,China
关键词:
毛囊重建皮肤细胞分离动物模型
Keywords:
Hair follicle reconstitution Skin cells isolation Animal model
分类号:
R 758.71
DOI:
10.13735/j.cjdv.1001-7089.201503065
文献标志码:
A
摘要:
目的 研究将新鲜分离的新生小鼠皮肤细胞移植到免疫缺陷鼠上有效重建毛囊的新技术。方法 新生C57小鼠取其皮肤,将表皮和真皮细胞分别分离并混合,做成高浓度的细胞悬液,移植到免疫缺陷鼠NU/NU,CB17 SCID,NOD SCID上,观察皮肤再生情况。结果 细胞移植2周之后,伤口处肉眼可见黑色皮肤形成,3周后长出大量毛发,8周后观察重建的毛发维持良好状态。没有移植细胞的对照观察到伤口愈合但没有毛发形成。组织学分析显示再生的皮肤含有成熟的毛囊和毛干并连有皮脂腺及真皮毛乳头,并且新形成的皮肤不仅有表皮层和真皮层,还有皮下脂肪层,表明移植的细胞可重建完整的皮肤。细胞移植到三种不同的免疫小鼠NU/NU,CB17 SCID和NOD SCID上,毛囊重建的结果相似。 结论 创建了一套分离和移植皮肤细胞的技术,可在免疫缺陷鼠上高效地重建毛发。该技术可以用于毛囊再生的研究,并具有潜在的临床应用价值。
Abstract:
Objective To investigated a new method to efficiently reconstitute hairproducing skin by grafting cells isolated from newborn mouse onto an immunosuppressive mouseMethods Epidermal and dermal cells were isolated from the neonatal C57 mice,and then combined together to form highdensity cell slurryCells were transplanted into NU/NU,CB17 SCID and NOD SCID mice to examine the ability of hair reconstitutionResults Black pigmented skin formed at 2 weeks after transplantationA lot of pigmented hair follicles appeared at 3 weeks,and kept normal structure at 8 weeks after graftingNo hair follicle was observed in the control wound site without cell transplantationHistological analysis of 3week and 8week grafts showed the reconstituted hair follicles had mature hair shafts associated with sebaceous gland and dermal papilla The reconstituted skin had epidermis ,dermis,and subcutaneous adipose layerA fullthickness skin was regeneratedConclusion An efficiency system for reconstituting hair follicles by grafting isolated skin cells was successfully establishedThis system can be applied for hair regeneration research and might be valuable for clinical application possibly

参考文献/References:

[1] Mahjour SB,Ghaffarpasand F,Wang H.Hair follicle regeneration in skin grafts:current concepts and future perspectives[J].Tissue Eng Part B Rev,2012,18(1):15-23.
[2] 魏文国,纪黎明,吴学清.毛发移植治疗秃发疗效分析[J].中国皮肤性病学杂志,2006,20(12):767-767
[3] Lichti U,Weinberg WC,Goodman L,et al.In vivo regulation of murine hair growth:insights from grafting defined cell populations onto nude mice[J].J Invest Dermatol,1993,101(1 Suppl):124S-129S.
[4] Lichti U,Anders J,Yuspa SH.Isolation and shortterm culture of primary keratinocytes,hair follicle populations and dermal cells from newborn mice and keratinocytes from adult mice for in vitro analysis and for grafting to immunodeficient mice[J].Nat Protoc,2008,3(5):799-810.
[5] Zheng Y,Du X,Wang W,et al.Organogenesis from dissociated cells:generation of mature cycling hair follicles from skinderived cells[J].J Invest Dermatol,2005,124(5):867-876.
[6] Zheng Y,Nace A,Chen W,et al.Mature hair follicles generated from dissociated cells:a universal mechanism of folliculoneogenesis[J].Dev Dyn,2010,239(10):2619-2626.
[7] 张潇予,王大光,朱丰,等.鼠毛囊重建初步研究[J].中华皮肤科杂志,2013,46(12):863-865.
[8] 肖顺娥,胡志奇,姜金豆,等.微型小室法毛囊重建模型的构建[J].中华实验外科杂志,2012,29(10):2079-2081.
[9] 孙锡金,胡志奇,苗勇.小鼠毛囊细胞注射移植实验研究[J].中华整形外科杂志,2012,28(10):4449.
[10] 侯春,胡志奇,孙锡金.毛囊细胞混合物移植诱导裸鼠毛囊样结构形成[J].南方医科大学学报,2007,15(8):886-888.
[11] Lee LF,Jiang TX,Garner W,et al.A Simplified Procedure to Reconstitute HairProducing Skin[J].Tissue Eng Part C Methods,2010,17(4):391-400.
[12] Yang CC,Cotsarelis G.Review of hair follicle dermal cells[J].J Dermatol Sci,2010,57(1):2-11.

备注/Memo

备注/Memo:
[基金项目]〖HTSS〗 山东省泰山学者海外特聘教授基金
更新日期/Last Update: 2015-11-20