[1]王文生,伍津津,雷霞.金雀异黄素对UVB氧化损伤HaCaT细胞的保护作用[J].中国皮肤性病学杂志,2014,(04):349-351.
 WANG Wen sheng,WU Jin jin,LEI Xia.The Protective Effect of Genistein on HaCaT Cells Against Oxidative Damage Caused by UVB[J].The Chinese Journal of Dermatovenereology,2014,(04):349-351.
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金雀异黄素对UVB氧化损伤HaCaT细胞的保护作用
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2014年04期
页码:
349-351
栏目:
论著
出版日期:
2014-04-20

文章信息/Info

Title:
The Protective Effect of Genistein on HaCaT Cells Against Oxidative Damage Caused by UVB
作者:
王文生伍津津雷霞
第三军医大学大坪医院皮肤科,重庆 400042
Author(s):
WANG WenshengWU JinjinLEI Xia
Department of Dermatology,Daping Hospital,Third Military Medical University,Chongqing 400042,China
关键词:
金雀异黄素UVB氧化反应HaCaT细胞
Keywords:
GenisteinUVBOxidative reactionHaCaT cell
分类号:
R 931.71
文献标志码:
A
摘要:
目的研究金雀异黄素(genistein,Gen)对中波紫外线(UVB)照射人角质形成细胞系HaCaT细胞所致氧化损伤的保护作用。方法体外培养HaCaT细胞,分成空白对照组(A组),25μmol/L金雀异黄素处理组(B组),UVB(30mJ/cm2)辐射组(C组),UVB辐射(30mJ/cm2)+25μmol/L金雀异黄素处理组(D组),MTT法检测细胞增殖,流式细胞仪检测活性氧(ROS)水平,生物化学法测定细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)活性。结果D组与C组相比,细胞存活率明显增加(C组62%,D组85%),细胞内ROS水平明显降低(C组237.21,D组120.57,抑制率为49%),细胞内SOD和GSHPx水平明显升高\[SOD:C组(180.55±8.34)nU/mL,D组(233.42±6.52)nU/mL;GSHPx:C组(310.72±22.52)U,D组(380.98±29.50)U\],两组之间的差别均有显著的统计学意义。结论Gen能增加细胞在UVB照射后的存活率,增加细胞内的抗氧化酶活性,降低细胞内ROS水平,对UVB照射所致人角质形成细胞氧化损伤具有保护作用。
Abstract:
ObjectiveTo investigate the effect of Genistein on HaCaT cells against oxidative damage caused by UVB.MethodsHaCaT cells were cultured in vitro.Cells were divided into 4 groups.A group:blank control(without UVB and Genistein);B group:25μM Genistein,without UVB;C group:30mJ/cm2 UVB,without Genistein;D group:30mJ/cm2 UVB,25μM Genistein.MTT was used to detect the cell viability,flow cytometry was used to detect intracellular ROS production.SOD/GSHpx test kit was used to test intracellular GSHPx,SOD activity.ResultsThe cell viability was higher,the ROS production was lower,and the activities of GSHPx,SOD were stronger in D group than those in C group.Cell viability:C group was 62%,D group was 85%.ROS:C group was 237.21,D group was 120.57,the inhibition ratio was 49%.SOD:C group was (180.55±8.34)nU/mL,D group was (233.42±6.52)nU/mL.GSHPx:C group was (310.72±22.52)U,D group was (380.98±29.50)U.There was statistically significant difference between the two groups.ConclusionGenistein could increase the cell viability after UVB irradiation,promote the activity of antioxidant enzyme,and reduce the intracellular ROS production.Genistein could protect HaCaT cells against oxidative damage caused by UVB.

参考文献/References:


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备注/Memo

备注/Memo:
基金项目]重庆市自然科学基金(CSTC.2010BB5021);国家教育部留学归国基金 [作者单位]第三军医大学大坪医院皮肤科,重庆 400042 [通讯作者]雷霞,E-mail:leixia1979@sina.com
更新日期/Last Update: 2014-04-20