[1]陈文琦,毕志刚,许惠娟.亚毒性剂量UVB照射和过表达p53对皮肤细胞光致癌相关基因表达的影响[J].中国皮肤性病学杂志,2013,(09):870-872.
 CHEN Wenqi,BI Zhigang,XU Huijuan.The Influence of Overexpression of Wild Type p53 and Repeated Subcytotoxic UVB Stress on Genes Expression Involved in Photocarcinogenesis in Human Skin Fibroblasts[J].The Chinese Journal of Dermatovenereology,2013,(09):870-872.
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亚毒性剂量UVB照射和过表达p53对皮肤细胞光致癌相关基因表达的影响
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《中国皮肤性病学杂志》[ISSN:1001-7089/CN:61-1197/R]

卷:
期数:
2013年09期
页码:
870-872
栏目:
论著
出版日期:
2013-09-01

文章信息/Info

Title:
The Influence of Overexpression of Wild Type p53 and Repeated Subcytotoxic UVB Stress on Genes Expression Involved in Photocarcinogenesis in Human Skin Fibroblasts
作者:
陈文琦1毕志刚2许惠娟1
1. 南京医科大学附属南京医院皮肤科,江苏 南京 210006; 2. 南京医科大学附属明基医院皮肤科,江苏 南京 210019
Author(s):
CHEN Wenqi1 BI Zhigang2 XU Huijuan1
(1. Department of Dermatology, Nanjing Hospital Affiliated to Nanjing Medical University, Nanjing 210006, China; 2. Department of Dermatology, BenQ Hospital Affiliated to Nanjing Medical University, Nanjing 210019, China)
关键词:
p53UVB光致癌人皮肤成纤维细胞
Keywords:
p53 UVB Photocarcinogenesis Human skin flbroblasts
分类号:
R 751
文献标志码:
A
摘要:
目的探讨亚毒性剂量的中波紫外线(UVB)照射和过表达p53对人皮肤成纤维细胞(HSF)在 光致癌相关基因表达的影响。方法以反复多次的亚毒性剂量UVB照射正常HSF细胞株和已 构建成功的p53稳定过表达HSF细胞株,定制实时定量PCR芯片,检测光致癌发生相关基 因的表达。结果UVB照射和过表达p53在促进衰老基因表达方面,UVB照射的作用更为显 著;过表达p53使抑凋亡基因bcl2下调,促凋亡基因bax上调,UVB照射则可加强过表 达p53的诱导凋亡作用;过表达p53可下调癌基因HIF1α,VEGF和hdm2的表达,UVB照 射可促进p53的肿瘤抑制作用。结论过表达p53可使p53依赖的细胞凋亡和抑制肿瘤作用 明显增强,亚毒性剂量的UVB还可加强这种作用,从而有助于抑制皮肤肿瘤发生。在UVB 作用下,过表达p53可能更倾向于诱导凋亡,而非衰老。
Abstract:
Objective To explore the effect of overexpression of p53 and repeated subcytotoxic UVB radiation on genes expression about photocarcinogenesis in human skin fibroblasts(HSFs). Methods HSFs and transfected wtp53 gene HSF p53 cells were treated with repeated subcytotoxic UVB radiation. A real time PCR array was performed to investigate mRNA level of genes involved in photocarcinogenesis. Results Repeated subcytotoxic UVB exposures induced genes expression involved in senescence prior to overexpression of p53. Transfected HSFp53 cells showed downregulation of bcl2, upregulation of bax, downregulation of HIF1α, VEGF and hdm2. Overexpression of p53 enhanced apoptosis and tumor repression activity and UVB radiation could even promoted them. Conclusion Overexpression of p53 can enhance p53related apoptosis and tumor repression activity with a tumorigenesis repression function, and repeated subcytotoxic UVB exposures can promote it. Overexpression of p53 may prefer to induce apotosis but not senescence under UVB radiation.

参考文献/References:

[1] 陈文琦,许惠娟,毕志刚.p53相关的细胞周期调控在中波紫外线诱导的皮肤成纤维细 胞早衰中的作用[J].中国皮肤性病学杂志,2009,23(9):541-543.
[2] 陈文琦,毕志刚,许惠娟.过表达p53基因和中波紫外线照射对人皮肤成纤维细胞衰 老的影响[J].中国皮肤性病学杂志,2012,26(6):488-491.
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[4] Sendoel A, Kohler I, Fellmann C, et al. HIF1 antagonizes p53mediated apoptosis through a secreted neuronal tyrosinase[J].Nature,2010,465 (7298):577-583.
[5] Yamakuchi M, Lotterman CD, Bao C, et al. P53induced microRNA107 inhibits HIF1 and tumor angiogenesis[J].Proc Natl Acad Sci U S A,2010,107 (14):6334-6339.
[6] Nardinocchi L, Puca R, Givol D, et al. HIPK2a therapeutical target to be(re) activated for tumor suppression: role in p53 activation and HIF1α inhibition[J].Cell Cycle,2010,9(7):1270-1275.
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备注/Memo

备注/Memo:
基金项目] 国家自然科学基金资助项目(30671894); 南京市卫生局青年科技人才启动项目 (QYK10135) [作者单位] 1. 南京医科大学附属南京医院皮肤科,江苏 南京 210006; 2. 南京医科大学附属明 基医院皮肤科,江苏 南京 210019 [通讯作者] 许惠娟,E-mail:xuhuij320@163.com
更新日期/Last Update: 2013-09-20